High Throughput Screening and Image Analysis for BioSciences
June 18-22, 2018
i3S - Instituto de Investigação e Inovação em Saúde | Rua Alfredo Allen, 208 | 4200-393 Porto, Portugal
Fundamentally practical, this course aims introducing the participants in experimental design, image acquisition, image and data analysis for high throughput (HT) experiments. These are essential skills when generating massive amount of imaged-based data in the biosciences field. Lectures will be given by specialists in the field and participants will learn how to use state of the art technology for HT experimentation and open-source software for image and data analysis. Attendants will get acquainted with use of automated liquid handling equipment and high content (HC) imagers such as IN Cell Analyzer (HCS microscope) and ImageStream (imaging flow cytometer). Interaction with HTS/HCS specialists will be fostered due to the restricted amount of participants.
ORGANIZATION
André Maia and María Gómez Lázaro
andre.maia@i3s.up.pt | maria.glazaro@ineb.up.pt
COURSE CONTENTS
- High throughput/content screening
- High content imaging systems
- Lab automation
- Target and drug discovery
- High content image analysis
- Data visualization and analysis
| Monday, 18 | Tuesday, 19 | Wednesday, 20 | Thursday, 21 | Friday, 22 | ||
| 09:00 | LAB Session III
Group 1 (IDEAS); Group 2 (ImgStream, Exp#2); Group 3 (Ensight, Exp#3); Group 4 (IN Cell, Exp#1) |
Lab Session VI Introduction to ImageJ all groups together Paulo Aguiar, i3S |
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| 09:30 | Target and Drug Discovery António Pombinho, i3S |
Microplate readers Mara Colzani, Perkin-Elmer |
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| 10:00 | ||||||
| 10:15 |
Light microscopy overview Paula Sampaio, i3S |
Lab automation and liquid handling David Ley, PAA |
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| 10:30 | Coffee-break | Coffee-break | ||||
| 11:00 | Coffee-break | Coffee-break | HTS/HCS Imaging Tools Enzo |
Introduction to ImageJ | ||
| 11:30 |
Light and Image Formation António Pereira, i3s |
LAB Session III Group 1 (IN Cell, Exp#1); Group 2 (EnSight, Exp#3); Group 3 (ImgStream, Exp#2); Group 4 (IDEAS) |
High Content Image Analysis Romain Guiet, EPFL |
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| 12:00 | Software installation | Data Analysis Hugo Botelho, BioISI, UL Software installation |
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| 12:15 | High Content Imaging Systems GE Healthcare |
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| 12:30 | Lunch | |||||
| 13:00 | Registration and Reception | Lunch | Lunch | Lunch | ||
| 13:30 | LAB Session IV CellProfiler all groups together Romain Guiet, EPFL |
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| 14:00 | High Content Imaging and Screening André Maia, i3S |
Hight Throughput Flow imagers Merck |
LAB Session III
Group 1 (Ensight, Exp#3); Group 2 (IN Cell, Exp#1); Group 3 (IDEAS); Group 4 (ImgStream, Exp#2) |
LAB Session VII HTM Explorer all groups together Hugo Botelho, BioISI, UL |
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| 14:30 | Cell Counting and High Throughput Image Analysis systems Nexcelom |
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| 14:45 |
Imaging in Flow Maria Lazaro, i3S |
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| 15:00 | ||||||
| 15:15 | Coffee-break | |||||
| 15:30 | Coffee-break | Coffee-break | Coffee-break | |||
| 15:45 | LAB Session II Group 1 (Fix cells, Exp#2); Group 2 (Celigo, demo); Group 3 (Janus, Exp#1); Group 4 (Liquidator, Exp#3) |
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| 16:00 | LAB Sessions Overview André Maia/Maria Lazaro |
LAB Session III Group 1 (ImgStream, Exp#2); Group 2 (IDEAS); Group 3 (IN Cell, Exp#1); Group 4 (Ensight, Exp#3) |
LAB Session V CellProfiler for Image Stream CellProfiler analyst all groups together Hue Summers, Swansea UK |
Coffee-break & Group Photo | ||
| 16:30 |
LAB session I Groups 1-2 (Experiment #1,#3)* Groups 3-4 (Experiment #2)* |
Experimental work discussion and questions André Maia/Maria Lazaro |
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| 16:45 | ||||||
| 17:00 | Groups 1-2 (Experiment #2)* Groups 3-4 (Experiment #1,#3)* |
Certificates | ||||
| 17:15 | Exame (optional) | |||||
| 17:30 | ECTS | |||||
| 18:00 |
Legenda
| Seminar | |
| Tecnology | |
| Practical |
* Experiments:
#1: HTS with HCS microscopy
#2: HTS with Imaging Cytometry
#3: HTS with microplate reader
André Maia, i3S, Porto
António Pereira, i3S, Porto
António Pombinho, i3S, Porto
Cristina Ferrás, i3S, Porto
Hugo Botelho, BioISI Lisboa
Huw Summers, College of Engineering, Swansea
María Gómez Lázaro, i3S, Porto
Marina Leite, i3S, Porto
Paula Sampaio, i3S, Porto
Romain Guiet, EPFL, Lausanne
Paulo Aguiar, i3S, Porto
REGISTRATION FEES:
Before 18th May 2018 | i3S members – 180€
Before 18th May 2018 | General registration - 225€
After 18th May 2018 | i3S members or General registration – 300€
Registration fee includes coffee breaks, lunches and course materials.
Limited number of participants (20)
Registration Deadline: 4th June 2018
Please make the payment only after the registration confirmation.
ECTS
This course is credited with 2 ECTS. If you are interested in ECTS please submit a copy of your ID card and the certificate of the higher degree on the registration form. Attendance to all sessions and to the final exam are mandatory.
In case you want more details, please contact events@i3s.up.pt
Equipment in demo
IN Cell Analyzer 2000 | GE Healthcare | More info >>
Celigo Image Cytometer | Nexcelom | More info >>
Ensight Multimode Plate Reader | Perkin Elmer | More info >>
ImageStream Imaging Flow Cytometer | Merck | More info >>
JANUS Automated Workstation | Perkin Elmer | More info >>
Liquidator 96 | Metler-Toledo | More info >>
Multidrop Combi | Thermo Scientific | More info >>
Cellometer | Nexcelom | More info >>