Perpétua Pinto do Ó
Microenvironments for New Therapies
Assistant Researcher
Perpetua Pinto-do-Ó is an Investigator at the INEB (Instituto de Engenharia Biomédica) & I3S (Instituto de Investigação e Inovação em Saúde, Universidade do Porto), where she leads a research team focused on  Stem Cell and Regenerative Biology. Perpetua differentiated background makes her a multifaceted scientist, enabling her to explore both basic and applied science from a multitude of perspectives. Shortly, after completing a degree in Biology and a three years’ research period in Pharmacology (Faculty of Medicine, Porto University/Department for Molecular Medicine, Karolinska Institute, Stockholm), Perpetua enrolled in the Gulbenkian PhD Program in Biology and Medicine (IGC, Oeiras, Portugal) in 1995. This was her opportunity to get in touch with Developmental Biology and Stem Cells, an area that she pursed and in which got a PhD degree from Umeå University, Sweden (1996-2002). Importantly, during this period, Perpetua was involved in the development of two cell lines, one derived from embryonic stem (ES) cells (A.K.A. HPC-7) and the other from adult bone marrow stem/progenitor cells (A.K.A. HPC-5), which proved as an in vitro immortalized source of a high-fidelity proxy for mouse and human hematopoietic stem/progenitor cells. These lines were henceforth used by several laboratories worldwide remaining up-today as a reference in the field accounting so far over 140 citations. Afterwards, she undertook a postdoctoral training at Instituto de Biologia Molecular e Celular-IBMC (2003-2006), which allowed her to develop further skills in molecular immunology. By the end of 2007, she became a researcher at INEB, where she launched a Stem Cell and Regenerative Biology research line.
Along with her long-time interest on basic stem cell biology mechanisms, Perpetua got interested on understanding the role stem/progenitor cells allocated to specific tissue/organs might have on the regeneration in the adult organism. Therefore, and as a counterpart to her experience with the highly regenerative hematopoietic system, her team at INEB initially focused on the heart (a poorly regenerative/repair system) as a model in this investigation. As a result, a series of in vivo and in vitro tools were explored, implemented and newly-created thereby allowing the establishment of a new research program on Cardiovascular Biology and Repair at the Institute. Currently, and following the recent resuming of the investigation on hematopoiesis, her lab is equipped with a highly regenerative and a poorly regenerative/repair working models. As a longstanding scientific purpose, Perpétua aims to establish a robust framework for comparative analysis studies across organ-systems focused on “the whether and how” stem/progenitor cells keep the homeostasis and functional restoration of distinct organ-systems.
Besides her activities as a researcher, Perpetua is currently the President of the Portuguese Society for Stem Cells and Cell Therapy (SPCE-TC) ( Moreover, she has been a lecturer on Stem Cells in Regenerative Therapies (2010-2014) at the Faculty of Engineering of the University of Porto (FEUP), and she is an Adjunct Professor at Instituto de Ciências Biomédicas Abel Salazar (ICBAS) of the University of Porto (2012-), where she is the the coordinator of  the "Stem Cell Biology" course. 
    P. Pinto-do-Ó Scientific identifiers: Scopus Author ID 6507474925;

Selected Publications
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate. Stem Cell Reports9(1):136-148, 2017. [Journal: Article] [IF: 7,3 (*)]
DOI: 10.1016/j.stemcr.2017.05.025 SCOPUS: 85021126795

Decellularized human colorectal cancer matrices polarize macrophages towards an anti-inflammatory phenotype promoting cancer cell invasion via CCL18. Biomaterials124:211-224, 2017. [Journal: Article] [CI: 5] [IF: 8,4 (*)]
DOI: 10.1016/j.biomaterials.2017.02.004 SCOPUS: 85013069713

Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult. Biomaterials104:52-64, 2016. [Journal: Article] [CI: 4] [IF: 8,4]
DOI: 10.1016/j.biomaterials.2016.06.062 SCOPUS: 84978822623

Hippo pathway effectors control cardiac progenitor cell fate by acting as dynamic sensors of substrate mechanics and nanostructure. ACS Nano8(3):2033-2047, 2014. [Journal: Article] [CI: 41] [IF: 12,9]
DOI: 10.1021/nn4058984 SCOPUS: 84896911335

Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms. Stem Cell Research and Therapy5(1):, 2014. [Journal: Article] [CI: 46] [IF: 3,4]
DOI: 10.1186/scrt394 SCOPUS: 84896713037

Sca-1+cardiac progenitor cells and heart-making: A critical synopsis. Stem Cells and Development23(19):2263-2273, 2014. [Journal: Review] [CI: 17] [IF: 3,7]
DOI: 10.1089/scd.2014.0197 SCOPUS: 84907432293

MIQuant - semi-automation of infarct size assessment in models of cardiac ischemic injury. PLoS ONE6(9):, 2011. [Journal: Article] [CI: 13] [IF: 4,1]
DOI: 10.1371/journal.pone.0025045 SCOPUS: 80053583980

Systemic delivery of bone marrow-derived mesenchymal stromal cells diminishes neuropathology in a mouse model of Krabbe's disease. Stem Cells29(11):1738-1751, 2011. [Journal: Article] [CI: 16] [IF: 7,8]
DOI: 10.1002/stem.724 SCOPUS: 80054957115

Hematopoietic progenitor/stem cells immortalized by Lhx2 generate functional hematopoietic cells in vivo. Blood99(11):3939-3946, 2002. [Journal: Article] [CI: 43] [IF: 9,6]
DOI: 10.1182/blood.V99.11.3939 SCOPUS: 0036624740

Expression of the LIM-homeobox gene LH2 generates immortalized Steel factor-dependent multipotent hematopoietic precursors. EMBO Journal17(19):5744-5756, 1998. [Journal: Article] [CI: 75] [IF: 13,2]
DOI: 10.1093/emboj/17.19.5744 SCOPUS: 0032190190