Biointerfaces and Nanotechnology

The Biointerfaces and Nanotechnology (BN) core facility is part of the i3S and INEB (an institution with experience and international recognition in the biomaterials and biomedical fields), and it is open to all academic researchers and industry on a fee-for-service basis. Our aim is the study of materials, materials surfaces and interfaces of materials with cells and tissues, going down to the micrometric and nanometric level through the development and the improvement of advanced physical, chemical, mechanical and structural characterization techniques. We are particularly focused on the fields of Biomaterials and Nano- and Regenerative Medicine. BN technical staff also provides advanced training and education in specialized areas.

 

Resources available include:

  • non-destructive chemical characterization (Fourier Transformed Infra-red Spectroscopy);
  • mechanical characterization (Dynamic Mechanical Analyzer and Rheometer);
  • nanoparticle characterization in solution (through the determination of particle size, particle charge, isoelectric point and melting point);
  • macro characterization of solids surface electric charge;
  • determination of the thickness of ultrathin films on reflective surfaces (Ellipsometry with Image);
  • quantification of surface wettability and surface free energy of solid surfaces (Contact Angle Measuring Device);
  • Analysis of surface topography, force spectroscopy and nanoindentation of synthetic and biological samples (Atomic Force Microscope couple with an Inverted Fluorescence Microscope).

Users are invited to follow up their analysis carried out at BN, which also provides advanced training, in the specific cases. A Quality System is implemented at BN, according to ISO standards and Good Laboratory Practices (GLP). This contributes to ensure the provision of strict procedures followed at BN core facility, thus enabling each technique to be performed according to high quality standards. BN was created in 2005 and the platform's daily activities are supported by our technical staff and its governance is assured by a Scientific Coordinator. Since one of our objectives is the continuous improvement, we look forward to receiving your input (info.bn@i3s.up.pt).

Applications

Atomic Force Microscope coupled with Inverted Fluorescence Microscope

Life and Health sciences:

  • 2D and 3D topographic imaging of molecules, cells, tissues and microorganisms;
  • Force Spectroscopy and Molecular recognition with the quantification of binding forces in ligand-receptor 
  • Cell-cell adhesion determination binding forces
  • Mechanical properties quantification in biological samples
  • Roughness and morphometric parameters quantification

Polymer Industry

  • Imaging and roughness measurements of surface

Biosensors and electronic industry

  • Imaging and roughness measurement of surfaces

Optical Contact Angle Measurement Device

  • Life and Health Sciences
  • Polymer Industry
  • Surface characterization (according to the chemical groups existing in the surface)
  • Agrochemical industry (the effectiveness of a sprayed liquid will be determined by the degree to which it coats and adheres to the surface
  • Shoes and clothes industry (wettability of leather and tissues surface)
  • Materials

Fourier Transformed Infra Red Spectrometer

  • Biology (adsorption of proteins, and other layers in a surface)
  • Materials (adorption of organic layers in ceramics, metals, ...)
  • Geology 
  • Film analysis
  • Identification of contaminants
  • Food industry (identification of trans fat content of manufactured food products)
  • Forensic sciences (identification of chemical information in residues of fibers, coatings, paints, ….)
  • Pharmaceutical field (formulation development, validation and quality control)

Ellipsometer with image

These equipment allows studies in different areas:

  • Biology (thickness of film proteins and natural polymers)
  • Biomaterials (quantification of thickness of thin oxide films formed on metallic biomaterials by surface treatment)
  • Refractive substrates (Films in a nanometric scale, namely self-assembled monolayers - SAMs,  deposited on a reflective substrate)
  • Semiconductors physics to microelectronics
  • Quality control (films metrology)
  • Flat panel display industry
  • Optical lenses
  • Photovoltaics thin films

 

Zetasizer Nano ZS

  • Study of nanoparticles (biomaterials, liposomes, …) formulation for health therapies: size and charge
  • Protein conformation data: size
  • Environmental strategies to address pollution: size
  • Formulation stability; Proteins and polymer characterization: size, charge and isoeletric point
  • Quantum dots analysis: size

Zeta Potential Electro Kinetic Analyzer 

  • Quantification of Zeta potential at different pH (applied to solid materials: powders, membranes, granules, plates, …)

Rheometer

It allows the application of 4 different modes of assays: viscometry, oscillation, creep and recovery and stress and relaxation in different areas:

  • Life sciences and Health (mechanics of biological tissues)
  • Materials Science (hydrogels,…)
  • Coatings and food Industry (paints and chocolate, respectively
  • Pharmaceutical and cosmetic industry (liquids, ointments, creams, pastes, ….)

Dynamic Mechanical Analyser

  • Materials Science (bulk solid, film, fiber,…), DMA applies different deformation modes: tension, compression, shear, single and/or dual cantilever bending and 3D point bending. The deformation modes can be applied resulting from changes in five experimental variables: temperature, time, frequency, force, and strain.
Resources

Atomic Force Microscope coupled with Inverted Fluorescence Microscope

The Atomic Force Microscope (AFM) is a surface characterization technique using a cantilever, based in the surface. This technique provides: i) 3D topographic imaging, surface roughness and morphometric studies, at the nanometric scale, ii) single molecule binding forces determination using Force Spectroscopy and molecular recognitions and iii) determination of mechanical properties (ex: stiffness, Apparent Young’s modulus, adhesion, deformation (indentation)) in physiological conditions. Our AFM (Pico Plus 5500, keysight Technologies, USA) performs the following modes: Contact, Non-Contact, and Intermittent-contact (Tapping® mode and Magnetic Acoustic Mode – MAC™). The MAC Mode™ is generally recognized as the AFM “gentle” mode and consists in a magnetic cantilever directly controlled by a magnetic field. It integrates the oscillating mode (Acoustic AC) and the phase imaging (digital) as well. This mode is indicated for measurements in fluids, being characterized by low contact forces applied in extremely delicate samples, such as: proteins, DNA, RNA, cells and other biological structures. The AFM is coupled to an Inverted Fluorescence Microscope (IFM) (Observer Z1, Zeiss, Germany). The system allows a variability of assays in biological samples. It has four objectives: 10X air, 40X oil, 63X oil and 100 X oil. It has filters to work with DAPI and eGFP fluorophores. It has Differential Interference Contrast (DIC). It does not permit to work with Phase contrast.

 

Responsible for the assays: Manuela Brás

 

Optical Contact Angle

The principle of this equipment is based in the optical contact angle (OCA) (OCA, Data Physics, USA) measurement between a liquid drop and a surface, being possible to use different measuring methods: pendant, sessile and inverted drop. Basically the drop is released from an automatic vertical needle through the sample, inside a chamber with temperature control. The contact angle is the angle, conventionally measured through the liquid, where a liquid-vapor interface meets a solid surface. It quantifies the wettability of a solid surface by a liquid via the Young’s equation. A given system of solid, liquid, and vapor at a given temperature and pressure has a unique equilibrium contact angle. This apparatus also allows the determination of the surface tension of pure liquids as well as with protein containing solutions. The equipment quantifies the free energy of a surface material, which is a very important parameter in material’s characterization. This technique also allows the determination of specific biomolecule’s affinity, such as proteins on a surface, by the calculation of the adhesion work.
Quotation: under request

 

 

Responsible for the assays: Manuela Brás

 

Dynamical Mechanical Analyzer

The DMA is used to study and characterize materials, being most useful for observing the mechanical properties and viscoelastic nature of polymers. The DMA can determine changes in sample properties applying different deformation modes: tension, compression, shear, single, dual cantilever bending and 3D point bending. The deformation modes can be applied resulting from changes in five experimental variables: temperature, time, frequency, force, and strain. The DMA uses samples that can be in bulk solid, film, fiber, gel, or very viscous liquid form.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Ellipsometer

Ellipsometry is an optical technique for investigating the dieletric properties (complex refractive index or dieletric function) of thin films. Ellipsometry measures the change of polarization of light upon reflection or transmission and compares it with a model. Our Ellipsometer (EP3, Accurion, Germany) is able to determine the refraction index and extinction coefficient index, and with these, the thickness of thin films covering reflecting surfaces. The equipment uses a 532 nm laser and allows the possibility of adjusting the angle of incidence for measurements, to carry out the following studies: i) Films in a nanometric scale, namely self-assembled monolayers - SAMs, polymeric films and ceramics, deposited on a reflective substrate; ii) Analysis of in situ and in real time material adsorption on the previously indicated films or reflective substrates (using a liquid cell); iii) Quantification of thickness of thin oxide films formed on metallic biomaterials by surface treatment.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Fourier Transformed Infra-Red Specctrometer

It is a vibrational spectroscopy technique that provides information about molecular structure, through the identification of functional groups and chemical bonds of molecules. It also allows quantification methods. The equipment may analyse solid samples (powders, dense materials, thick films, gels) and liquids. In case of FTIR spectroscopy, the available accessories allow for different analytical methods, namely:

  • Transmittance
  • ATR (Attenuated Total Reflectance)
  • IRRAS (Infra- Red Reflectance Absorption Spectroscopy) for films with a monolayer thickness, in reflective surfaces


Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal/ Manuela Brás

 

Zetasizer Nano ZS

Zetasizer Nano ZS has the ability to measure characteristics of particles or molecules dispersed in a liquid medium, namely particle size and zeta potential, in a wide range of concentrations. The Zetasizer Nano ZS is equipped with a MPT-2 autotitrator, a 633 nm laser and allows the temperature control from 10 to 90 ºC.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Rheometer Kinexus

The Kinexus Rheometer is used to study and characterize rheological properties of materials, being most useful for observing the viscosity and viscoelasticity of polymers. The Rheometer allows the application of 4 different modes of assays: viscometry, oscillation, creep and recovery and stress and relaxation. It determines then, changes in sample properties resulting from changes in external conditions applied, such as stress, strain, timescale and temperature. The rheometer uses samples that can be in bulk solid, molten, semi-solid (such as pastes, creams and gels) or viscous to dilute liquid solutions.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Zeta Potential Electro Kinetic Analyzer

The Electro Kinetic Analyzer (EKA) can be used to investigate the zeta potential of macroscopic solids based on a streaming potential and streaming current measurement. The zeta potential is related to the surface charge at a solid/liquid interface and it is a powerful indicator for the surface chemistry and liquid phase adsorption processes. It is therefore an interfacial property of great importance to understand the behaviour of solid materials in several biological/technical processes. The EKA instrument has five different measuring cells for flexible sample mounting, which may accommodate macroscopic solids of almost any size and shape, namely planar solid samples, films, fibers, foils, granular particles and powders.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Services

A Quality System is implemented at BN, according to ISO standards and Good Laboratory Practices (GLP). This contributes to ensure the provision of strict procedures followed at BN facility, thus enabling each technique to be performed according to high quality standards. Prices are under revision.

Training

Past Training Actions

Workshop in Atomic Force Microscopy coupled to Inverted Fluorescence Microscopy
15-17 November 2017 | i3S, Lab 222 S2 and Room 2

Atomic Force Microscopy (AFM) has revealed as a powerful tool to study human pathology, in fields ranging from cancer, cardiovascular and blood diseases, since it is suitable to perform studies on different molecules, cell/ tissue types at physiological conditions. The main principle is based in the interatomic forces stablished between a probe tip and the sample causing the cantilever to deflect as the samples’ surface topography changes. A laser light reflected from the back of the cantilever measures the deflection of the cantilever. In this lab session the participants will be introduced to the AFM/IFM techniques, having the opportunity to learn about the determination of morphometric parameters to the characterization of biomechanical properties of cells. As well, students will learn how to use different software for the analysis of the data arising from these studies. Applets software will be used to analyze the Thermal tune data to obtain the force constant of the cantilevers and to analyze AFM force distance curves, to calculate the mechanical properties of the samples.

 

Output

Publications

 

Karabiyik C, Fernandes R, Figueiredo FR, Socodato R, Lambertsen KL, Relvas JB, Santos SD. 2017 Neuronal Rho GTPase Rac1 elimination confers neuroprotection in a mouse model of permanent ischemic stroke. Brain Pathol.

 

Leiro, V., Garcia, J.P., Moreno, P., Spencer, A.P., Fernandez-Villamarin, M., Riguera, R., Fernandez-Megia, E., Pêgo, A.P. 2017 “Biodegradable PEG-GATGE dendritic block copolymers: Synthesis and Biofunctionality Assessment as Vectors of siRNA”. Journal of Materials Chemistry B. 5, 4901-4917.

 

Costa F, Sousa DM, Lamghari M, Gomes P, Martins MCL. N-acetylcysteine-functionalization coating avoids bacterial adhesion and biofilm formation. Scientific Reports. 2017; 7;17374; 1-13

 

Felgueiras HP, Wang LM; Ren KF; Querido M; Jin Q; Barbosa MA; Ji J; Martins MCL. Octadecyl chains immobilized onto hyaluronic acid coatings by thiol-ene "click chemistry" increase the surface antimicrobial properties and prevent platelet adhesion and activation to polyurethane. ACS Applied Materials and Interfaces. 2017; 9 (9) 7979–7989

 

Seabra CL, Nunes C, Gomez-Lazaro, Correia M, Machado JC, Gonçalves IC, ReisCA,Reis S, Martins MCL.Docosahexaenoic acid loaded lipid nanoparticles with bactericidal activity against Helicobacter pylori. International Journal of Pharmaceutics. 2017; 519; 128-137

 

J.R. Dias, S. Baptista-Silva, C.M.T. de Oliveira, A. Sousa, A.L. Oliveira, P.J. Bártolo, P.L. Granja, In situ crosslinked electrospun gelatin nanofibers for skin regeneration, European Polymer Journal, Volume 95, 2017, Pages 161-173

 

Castro F, Pinto ML, Silva AM, Pereira CL, Teixeira GQ, Gomez-Lazaro M, Santos SG, Barbosa MA, Gonçalves RM, Oliveira MJ. Pro-inflammatory chitosan/poly(γ-glutamic acid) nanoparticles modulate human antigen-presenting cells phenotype and revert their pro-invasive capacity. Acta Biomater. 2017 Nov;63:96-109.

 

Antunes JC, Pereira CL, Teixeira GQ, Silva RV, Caldeira J, Grad S, Gonçalves RM, Barbosa MA, Poly(γ-glutamic acid) and poly(γ-glutamic acid)-based nanocomplexes enhance type II collagen production in intervertebral disc.J Mater Sci Mater Med. 2017 Jan;28(1):6

 

Andreia M. Silva, Maria I. Almeida, José H. Teixeira, André F. Maia, George A. Calin, Mário A. Barbosa, Susana G. Santos 2017, Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment. Under minor revision in Scientific Reports.

 

Teixeira GQ, Leite Pereira C, Castro F, Ferreira JR, Gomez-Lazaro M, Aguiar P, Barbosa MA, Neidlinger-Wilke C, Goncalves RM, Anti-inflammatory Chitosan/Poly-?-glutamic acid nanoparticles control inflammation while remodeling extracellular matrix in degenerated intervertebral disc. Acta Biomater. 2016; 42:168-79

 

Antunes JC, Pereira CL, Teixeira GQ, Silva RV, Caldeira J, Grad S, Gonçalves RM, Barbosa MA, Poly(g-glutamic acid) and poly(g-glutamic acid)-based nanocomplexes enhance type II collagen production in intervertebral disc, J Mater Sci Mater Med. 2017;28(1):6.

 

I.C. Gonçalves, A. Magalhães, A.M.S. Costa, J.R. Oliveira, P.C. Henriques, P. Gomes, C.A. Reis, M.C.L. Martins. Bacteria-targeted biomaterials: glycan-coated microspheres to bind Helicobacter pylori. Acta Biomaterialia 2016; 33:40–50

 

Daniel M. Vasconcelos, Raquel M Gonc?alves, Catarina R Almeida, Ine?s O Pereira, Marta I Oliveira, Nuno Neves, Andreia M Silva, Anto?nio C Ribeiro, Carla Cunha, Ana R Almeida, Cristina C. Ribeiro, Ana M. Gil, Elisabeth Seebach, Katharyna L. Kynast, Wiltrud Richter, Meriem Lamghari, Susana G Santos and Ma?rio A Barbosa, "Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration”, Biomaterials 2016;111:163-178.

 

Cunha-Reis C, Machado A, Barreiros L, Araújo F, Nunes R, Seabra V, Ferreira D, Segundo MA, Sarmento B, das Neves J. Nanoparticles-in-film for the combined vaginal delivery of anti-HIV microbicide drugs. J Control Release. 2016 Dec 10;243:43-53.

 

Braz L Grenha A, Ferreira D, Rosa da Costa AM, Gamazo C, Sarmento B, Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization. Int J Biol Macromol. 2016 Dec 31;96:786-797.

 

Abreu CM, Paula HC, Seabra V, Feitosa JP, Sarmento B, de Paula RCSynthesis and characterization of non-toxic and thermo-sensitive poly(N-isopropylacrylamide)-grafted cashew gum nanoparticles as a potential epirubicin delivery matrix. Carbohydr Polym. 2016 Dec 10; 154: 77-85.

 

Gomes CP, Varela-Moreira A, Leiro V, Lopes CD, Moreno PM, Gomez-Lazaro M, Pêgo AP. A high-throughput bioimaging study to assess the impact of chitosan-based nanoparticle degradation on DNA delivery performance. Acta Biomater. 2016 Dec;46:129-140.

 

Rodrigues F, Alves AC, Nunes C, Sarmento B, Amaral MH, Reis S, Oliveira MB. Permeation of topically applied caffeine from a food by-product in cosmetic formulations: Is nanoscale in vitro approach an option? Int J Pharm. 2016 Nov 20;513(1-2):496-503.

 

Fonte P, Andrade F, Azevedo C, Pinto J, Seabra V, van de Weert M, Reis S, Sarmento B. Effect of the Freezing Step in the Stability and Bioactivity of Protein-Loaded PLGA Nanoparticles Upon Lyophilization. Pharm Res. 2016 Nov;33(11):2777-93.

 

Sgorla D, Bunhak ÉJ, Cavalcanti OA, Fonte P, Sarmento B. Exploitation of lipid-polymeric matrices at nanoscale for drug delivery applications. Expert Opin Drug Deliv. 2016 Sep;13(9):1301-9

 

Laranjeira MS, Moço A, Ferreira J, Coimbra S, Costa E, Santos-Silva A, Ferreira PJ, Monteiro FJ Different hydroxyapatite magnetic nanoparticles for medical imaging: Its effects on hemostatic, hemolytic activity and cellular cytotoxicity. Colloids Surf B Biointerfaces. 2016

 

Machado A, Cunha-Reis C, Araújo F, Nunes R, Seabra V, Ferreira D, das Neves J, Sarmento B Development and in vivo safety assessment of tenofovir-loaded nanoparticles-in-film as a novel vaginal microbicide delivery system. Acta Biomater. 2016 Oct 15;44:332-40.

 

Felgueiras HP,Murthy NS, , Brás MM, Migonney V, Kohn J, Competitive Adsorption of Plasma Proteins Using Quartz Crystal Microbalance. ACS Appl Mater Interfaces, 2016,

 

Ribeiro M, Ferraz MP, Monteiro FJ, Fernandes MH, Beppu MM, Mantione D, Sardon H. Antibacterial silk fibroin/nanohydroxyapatite hydrogels with silver and gold nanoparticles for bone regeneration. Nanomedicine. 2016 Aug 31; 13(1):231-239.

 

González-Delgado JA, Castro PM, Machado A, Araújo F, Rodrigues F, Korsak B, Ferreira M, Tomé JP, Sarmento B. Hydrogels containing porphyrin-loaded nanoparticles for topical photodynamic applications. Int J Pharm. 2016 Aug 20;510(1):221-31.

 

das Neves J, Nunes R, Rodrigues F, Sarmento B. Nanomedicine in the development of anti-HIV microbicides. Adv Drug Deliv Rev. 2016 Aug 1;103:57-75. doi: 10.1016/j.addr.2016.01.017

 

Costa A, Pinheiro M, Magalhães J, Ribeiro R, Seabra V, Reis S, Sarmento B. The formulation of nanomedicines for treating tuberculosis. Adv Drug Deliv Rev. 2016 Jul 1;102:102-15.

 

Lopes CD, Oliveira H, Estevão I, Pires LR, Pêgo AP. In vivo targeted gene delivery to peripheral neurons mediated by neurotropic poly(ethylene imine)-based nanoparticles. Int J Nanomedicine. 2016 Jun 8;11:2675-83.

 

Vieira AC, Chaves LL, Pinheiro M, Ferreira D, Sarmento B, Reis S. Design and statistical modeling of mannose-decorated dapsone-containing nanoparticles as a strategy of targeting intestinal M-cells. Int J Nanomedicine. 2016 Jun 3;11:2601-17

 

Gomes MJ, Fernandes C, Martins S, Borges F, Sarmento B.Tailoring Lipid and Polymeric Nanoparticles as siRNA Carriers towards the Blood-Brain Barrier - from Targeting to Safe Administration. J Neuroimmune Pharmacol. 2016 May 21

 

Araújo F, Shrestha N, Gomes MJ, Herranz-Blanco B, Liu D, Hirvonen JJ, Granja PL, Santos HA, Sarmento B. In vivo dual-delivery of glucagon like peptide-1 (GLP-1) and dipeptidyl peptidase-4 (DPP4) inhibitor through composites prepared by microfluidics for diabetes therapy. Nanoscale. 2016 May 19;8(20):10706-13.

 

Fonte P, Lino PR, Seabra V, Almeida AJ, Reis S, Sarmento B. Annealing as a tool for the optimization of lyophilization and ensuring of the stability of protein-loaded PLGA nanoparticles. Int J Pharm. 2016 Apr 30;503(1-2):163-73

 

Fonte PReis SSarmento BFacts and evidences on the lyophilization of polymeric nanoparticles for drug delivery. J Control Release.2016 Mar 10;225:75-86

 

 

da Silva SB, Ferreira D, Pintado M, Sarmento B. Chitosan-based nanoparticles for rosmarinic acid ocular delivery--In vitro tests. Int J Biol Macromol. 2016 Mar;84:112-20.

 

Fonte P, Araújo F, Seabra V, Reis S, van de Weert M, Sarmento B. Co-encapsulation of lyoprotectants improves the stability of protein-loaded PLGA nanoparticles upon lyophilization. Int J Pharm. 2015 Dec 30;496(2):850-62.

 

Salgado, C. L., Grenho, L., Fernandes, M. H., Colaco, B. J., & Monteiro, F. J. 2016. Biodegradation, biocompatibility, and osteoconduction evaluation of collagen-nanohydroxyapatite cryogels for bone tissue regeneration. J Biomed Mater Res A, 104(1), 57-70.

 

Oliveira MI, Pinto ML, Gonçalves RM, Martins MC, Santos SG, Barbosa MA. Adsorbed Fibrinogen stimulates TLR-4 on monocytes and induces BMP-2 expression. Acta Biomater. 2017 Feb;49:296-305.

 

A 3D in vitro model to explore the inter-conversion between epithelial and mesenchymal states during EMT and its reversion.Bidarra SJ, Oliveira P, Rocha S, Saraiva DP, Oliveira C, Barrias CC. Sci Rep. 2016 Jun 3;6:27072

 

Andreia Almeida, Daniella Silva, Virginia Gonçalves, Bruno Sarmento. Synthesis and characterization of chitosan-grafted-polycaprolactone micelles for modulate intestinal paclitaxel delivery. Drug Delivery and Translational Research, pp 1–11

 

I.C. Gonçalves, A. Magalhães, A.M.S. Costa, J.R. Oliveira, P.C. Henriques, P. Gomes, C.A. Reis, M.C.L. Martins. Bacteria-targeted biomaterials: glycan-coated microspheres to bind Helicobacter pylori. Acta Biomaterialia 2016; 33:40–50

 

Costa F, Maia S, Gomes J, Gomes P and Martins MCL. Dhvar5 antimicrobial peptide (AMP) immobilization strategy has a high impact on the decrease of surface bacterial colonization. Biomaterials. 2015. 52:531-538. (IF: 8.312; CIT: 2).

 

Costa F, Maia S, Gomes J, Gomes P and Martins MCL. Characterization of hLF1-11 immobilization onto chitosan ultrathin films, and its effects on antimicrobial activity. Acta Biomater. 2014. 10(8):3513-21.

 

Freitas SC, Maia S, Figueiredo AC, Gomes P, Pereira PJB, Barbosa MA, Martins MCL. Selective albumin-binding surfaces modified with a thrombin inhibiting peptide. Acta Biomaterialia. 2014. 10:1227–1237.

 

Francisca Araújo, Neha Shrestha, Mohammed-Ali Shahbazi, Pedro Fonte, Ermei Mäkilä, Jarno Salonen, Jouni Hirvonen, Pedro L. Granja, Hélder A. Santos, Bruno Sarmento, The impact of nanoparticles on the mucosal translocation and transport of GLP-1 across the intestinal epithelium, Biomaterials, 35, 9192-9297, 2014

 

José das Neves, Francisca Araújo, Fernanda Andrade, Mansoor Amiji, Maria Fernanda Bahia, Bruno Sarmento, Biodistribution and pharmacokinetics of dapivirine-loaded nanoparticles after vaginal delivery in mice, Pharmaceutical Research, 31, 1834-1845, 2014

 

Nogueira F. et al, Effect of gastric environment on Helicobacter pylori adhesion to a mucoadhesive polymer, Acta Biomaterialia 9 2013, 5208–5215

 

Amaral IF., et al, Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII7–10), Acta Biomaterialia 9, 2013, 5643–5652

 

 

Partnerships

Participation in International Networks

ARBRE/ MOBIEU
Association of Resources for Physical Research in Europe/ Molecular Biophysics in Europe