High Throughput Screening and Image Analysis for BioSciences

 

June 18-22, 2018
i3S - Instituto de Investigação e Inovação em Saúde  | Rua Alfredo Allen, 208 | 4200-393 Porto, Portugal

 

Fundamentally practical, this course aims introducing the participants in experimental design, image acquisition, image and data analysis for high throughput (HT) experiments. These are essential skills when generating massive amount of imaged-based data in the biosciences field. Lectures will be given by specialists in the field and participants will learn how to use state of the art technology for HT experimentation and open-source software for image and data analysis. Attendants will get acquainted with use of automated liquid handling equipment and high content (HC) imagers such as IN Cell Analyzer (HCS microscope) and ImageStream (imaging flow cytometer). Interaction with HTS/HCS specialists will be fostered due to the restricted amount of participants.

 

ORGANIZATION
André Maia and María Gómez Lázaro
andre.maia@i3s.up.pt | maria.glazaro@ineb.up.pt

 

COURSE CONTENTS
- High throughput/content screening
- High content imaging systems
- Lab automation
- Target and drug discovery
- High content image analysis
- Data visualization and analysis

 

Program


    Monday, 18 Tuesday, 19 Wednesday, 20 Thursday, 21 Friday, 22
           
09:00     LAB Session III Group 1 (IDEAS);
Group 2 (ImgStream, Exp#2);
Group 3 (Ensight, Exp#3);
Group 4 (IN Cell, Exp#1)
Lab Session VI
Introduction to ImageJ
all groups together
Paulo Aguiar, i3S
09:30 Target and Drug Discovery
António Pombinho, i3S
Microplate readers
Mara Colzani, Perkin-Elmer
10:00
10:15 Light microscopy overview
Paula Sampaio, i3S
Lab automation and liquid handling
David Ley, PAA
10:30 Coffee-break Coffee-break
11:00 Coffee-break Coffee-break HTS/HCS Imaging Tools
Enzo
Introduction to ImageJ
11:30 Light and Image Formation
António Pereira, i3s
LAB Session III
Group 1 (IN Cell, Exp#1);
Group 2 (EnSight, Exp#3);
Group 3 (ImgStream, Exp#2);
Group 4 (IDEAS)
High Content Image Analysis
Romain Guiet, EPFL
12:00 Software installation Data Analysis
Hugo Botelho, BioISI, UL
Software installation
12:15 High Content Imaging Systems
GE Healthcare
12:30 Lunch
13:00 Registration and Reception Lunch Lunch Lunch
13:30 LAB Session IV
CellProfiler
all groups together
Romain Guiet, EPFL
14:00 High Content Imaging and Screening
André Maia, i3S
Hight Throughput Flow imagers
Merck
LAB Session III Group 1 (Ensight, Exp#3);
Group 2 (IN Cell, Exp#1);
Group 3 (IDEAS);
Group 4 (ImgStream, Exp#2)
LAB Session VII
HTM Explorer
all groups together
Hugo Botelho, BioISI, UL
14:30 Cell Counting and High Throughput Image Analysis systems
Nexcelom
14:45 Imaging in Flow
Maria Lazaro, i3S
15:00
15:15 Coffee-break
15:30 Coffee-break Coffee-break Coffee-break
15:45 LAB Session II
Group 1 (Fix cells, Exp#2);
Group 2 (Celigo, demo);
Group 3 (Janus, Exp#1);
Group 4 (Liquidator, Exp#3)
16:00 LAB Sessions Overview
André Maia/Maria Lazaro
LAB Session III
Group 1 (ImgStream, Exp#2);
Group 2 (IDEAS);
Group 3 (IN Cell, Exp#1);
Group 4 (Ensight, Exp#3)
LAB Session V
CellProfiler for Image Stream CellProfiler analyst
all groups together
Hue Summers, Swansea UK
Coffee-break &  Group Photo
16:30 LAB session I
Groups 1-2 (Experiment #1,#3)*
Groups 3-4 (Experiment #2)*
Experimental work discussion and questions
André Maia/Maria Lazaro
16:45
17:00 Groups 1-2 (Experiment #2)*
Groups 3-4 (Experiment #1,#3)*
Certificates
17:15 Exame (optional)
17:30     ECTS
18:00          

 

Legenda

  Seminar
  Tecnology
  Practical


* Experiments:
#1: HTS with HCS microscopy
#2: HTS with Imaging Cytometry
#3: HTS with microplate reader

 

Speakers

André Maia, i3S, Porto
António Pereira, i3S, Porto
António Pombinho, i3S, Porto
Cristina Ferrás, i3S, Porto
Hugo Botelho, BioISI Lisboa
Huw Summers, College of Engineering, Swansea
María Gómez Lázaro, i3S, Porto
Marina Leite, i3S, Porto
Paula Sampaio, i3S, Porto
Romain Guiet, EPFL, Lausanne
Paulo Aguiar, i3S, Porto

Registration

REGISTRATION FEES:
Before 18th May 2018 | i3S members – 180€
Before 18th May 2018 | General registration - 225€
After 18th May 2018 | i3S members or General registration – 300€

 

Registration fee includes coffee breaks, lunches and course materials.
Limited number of participants (20)
Registration Deadline: 4th June 2018

 

 

Please make the payment only after the registration confirmation.

 

 

ECTS
This course is credited with 2 ECTS. If you are interested in ECTS please submit a copy of your ID card and the certificate of the higher degree on the registration form. Attendance to all sessions and to the final exam are mandatory.

In case you want more details, please contact events@i3s.up.pt 

 

 

Sponsors

MerckPerkin Elmer ILC Instrumentos de Laboratório e Científicos Ldapaa peak analysis & automation PVL GE HealthcareNexcelom Bioscience  Palex  Enzo     

 

Lab Sessions

Equipment in demo

 

IN Cell Analyzer 2000 | GE Healthcare | More info >>
Celigo Image Cytometer | Nexcelom | More info >>
Ensight Multimode Plate Reader | Perkin Elmer | More info >>
ImageStream Imaging Flow Cytometer | Merck  | More info >>
JANUS Automated Workstation | Perkin Elmer  | More info >>
Liquidator 96 | Metler-Toledo  | More info >>
Multidrop Combi | Thermo Scientific | More info >>
Cellometer | Nexcelom | More info >>