First Meeting of the Portuguese Network on Extracellular Vesicles (PNEV)
13-14 September 2021 | i3S, Porto, Portugal
Welcome,
This will be the first meeting of the Portuguese Network on Extracellular Vesicles (PNEV), aiming to bring together researchers and students working in Portugal in the field of Extracellular Vesicles (EVs) in Health and Disease. The meeting, unfortunately, had to be postponed for the past year, due to the pandemic of Covid-19, but we are now in the position to plan it as a face-to-face meeting for September 2021.
It is expected that this meeting, which will be held at i3S in the beautiful city of Porto - Portugal, will help to establish national collaborations in this area of research, promoting scientific and technical advances in this research field. The program will consist of four invited talks, by internationally recognized speakers from various EV societies or associations and by several selected oral presentations. The meeting is organized in four sessions:
I) Methods for EVs isolation and characterization;
II) EVs in infection and immunity;
III) EVs in cancer;
IV) EVs in tissue regeneration and degeneration.
The event will also include a general meeting of the PNEV members, during which future directions of the PNEV will be discussed, together with a debate on how to collaborate with other networks, associations or societies in the field of EVs around the world. A social program will also be included, to promote interaction between all attendees.
We sincerely hope that this meeting will contribute to promoting outstanding research on EVs in Portugal.
The organizers,
Anabela Cordeiro da Silva, M. Helena Vasconcelos, Susana Santos
(PNEV coordinators)
Organization
ORGANIZING COMMITTEE:
Anabela Cordeiro da Silva, i3S and FFUP
M. Helena Vasconcelos, i3S and FFUP
Susana Santos, i3S and ICBAS
SCIENTIFIC COMMITTEE:
Alexandra Fernandes, FCT-NOVA
Ana Gabriela Henriques, iBiMED
Anabela Cordeiro da Silva, i3S
António Pedro Alves Matos, CiiEM
António Salgado, ICVS
Dina Simes, CCMAR
Dora Brites, iMed.ULisboa
Duarte Toubarro, Univ. Açores
Henrique Girão, iCBR
Hernando Del Portillo, Universitat de Barcelona, Spain (Representative of GEIVEX)
Inês Pinto, INL
Júlia Costa, ITQB, NOVA
Lino Ferreira, FMUC
Lorraine O’ Driscoll, Trinity College Dublin, Ireland (Representative of ISEV)
M. Helena Vasconcelos, i3S
Maria João Amorim, IGC
Rune Matthiesen, CEDOQ
Susana Santos, i3S
Teresa Gonçalves, CNC
Verónica Romão, INESC-MN
Program
DAY 1 | 13th September
8:50 REGISTRATION
9:00 – 9:10 OPENING SESSION
Celso Reis, Representative of the i3S Board of Directors
9:10 – 11:05 SESSION I: METHODS FOR EVs ISOLATION AND CHARACTERIZATION
Chair: António Salgado
9:10 – 09:50 Invited talk: Contamination of extracellular vesicle preparations or biomolecular corona around around extracellular vesicles? | ONLINE
Edit I Buzas, Semmelweis University, HCEMM-SU and ELKH-SE Immune-Proteogenomics Research Group, Hungary
9:50 – 10:05 Selected oral presentation 01: The potential of a magnetoresistive biochip platform for the specific detection of cell-derived microvesicles
Elisabete Fernandes, INL - International Iberian Nanotechnology Laboratory, Portugal
10:05 – 10:20 Selected oral presentation 02: Separation of extracellular vesicles from human cerebrospinal fluid
Júlia Costa, ITQB NOVA, Portugal
10:20 – 10:35 Selected oral presentation 03: Characterization and proteomic analysis of EVs from dogs with Canine Leishmaniasis
Sofia Esteves, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do
Porto, Porto, Portugal
10:35 – 11:05 Speed talks presentations
ST1. Proteomic comparison of blood-derived exosomes isolated using precipitation and column-based approaches
Tânia Soares Martins, Institute of Biomedicine (iBiMED), University of Aveiro, Portugal
ST2. Nature-inspired photonic sensor for microRNAs in cancer-associated extracellular vesicles
Raquel Vaz, BioMark Sensor Research, University of Coimbra, Portugal
ST3. Urinary Extracellular Vesicles: A comparison between isolation methods
Ana Teixeira-Marques, Research Center of IPO, Portugal
ST4. Characterization of filamentous fungi extracellular vesicles obtained using a novel protocol
Chantal Fernandes, Center for Neurosciences and Cell Biology, University of Coimbra, Portugal
ST5. Optimizing a freeze-drying methodology for mammal extracellular vesicles
Cristina Fornaguera, Institut Químic de Sarrià (IQS), Universitat Ramon Llull (URL), Spain
11:05 – 11:10 Sponsor talk: New advances in the direct characterization of extracelular vesicles from any biological sample | ONLINE
Aitor Gonzalez Granja, Business Line Manager Life Science at Izasa Scientific
11:10 – 11:40 Coffee-Break
11:40 – 13:30 SESSION II: EVs IN INFECTION AND IMMUNITY
Chair: Anabela Cordeiro-da-Silva
11:40 – 12:20 Invited talk: Unveiling the role of reticulocyte-derived exosomes in natural infections of Plasmodium vivax
Hernando Del Portillo, ISGlobal Institute for Global Health, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain and IGTP Institut d’Investigació Germans Trias i Pujol, Badalona, Spain
12:20 – 12:35 Selected oral presentation 04: Cyanobacterial extracellular vesicles as promising protein delivery/display systems for fish-related applications
Jorge Matinha-Cardoso, i3S - Instituto de Investigação e Inovação em Saúde,
Universidade do Porto, Portugal
12:35 – 12:50 Selected oral presentation 05: Identification of players and mechanisms involved in the biogenesis of cyanobacterial extracellular vesicles
Delfim Cardoso, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do
Porto
12:50 – 13:05 Selected oral presentation 06: Isolation of extracellular vesicles from entomopathogenic nematode: the new virulence factor in pathogen Infection
Duarte Toubarro, Universidade dos Açores, Portugal
13:05 – 13:30 Speed talks presentations
ST6. What if bacterial extracellular vesicles were an alternative copper-efflux mechanism?
Steeve Lima, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do
Porto, Porto, Portugal
ST7. Extracellular Vesicles from the parasitic trematodes Fasciola hepatica and Dicrocoelium dendriticum have different immunomodulatory properties in human THP-1 macrophages
Christian M. Sánchez-López, Universitat de València, Spain
ST8. Cyanobacterial extracellular vesicles: biogenesis mechanisms, biological roles, and biotechnological applications
Paulo Oliveira, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do
Porto, Portugal
ST9. Unveiling the role of extracelullar vesicles secreted by Giardia lamblia on innate immune response
Clarissa Perez Faria, Center for Neuroscience and Cell Biology, University of Coimbra, Portugal
ST10. Proteomic characterization of Extracellular Vesicles recovered from acute patients of Human Leishmaniasis in the context of HIV co-infection and Immunosuppression associated to solid organ transplants.
Inês Costa, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do
Porto, Portugal
13:30 – 14:30 Lunch Break
14:30 - 16:25 SESSION III: EVs IN CANCER
Chair: Helena Vasconcelos
14:30 – 15:10 Invited talk: EV-based biomarkers for cancer | ONLINE
An Hendrix, Laboratory of Experimental Cancer Research, University of Gent, Belgium
15:10 – 15:25 Selected oral presentation 07: Translating the glycan code of extracellular vesicles in gastric cancer cell communication
Ana Magalhães, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do
Porto, Portugal
15:25 – 15:40 Selected oral presentation 08: CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
Víctor Toribio, CBMSO, UAM, Spain
15:40 – 15:55 Selected oral presentation 09: Chitinase 3-like-1 found in the cargo of large extracellular vesicles released by human macrophages: potential therapeutic target for pancreatic cancer
Cristina P.R. Xavier, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal
15:55 – 16:25 Speed talks presentations
ST11. The role of truncated O-Glycans in cellular communication and cancer cell behaviour
Daniela Freitas, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal
ST12. Drug sequestration by Extracellular Vesicles shed by multidrug resistant cancer cells: is there a role for P-glycoprotein in this process?
Rita Rebelo, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto; Faculty of Medicine of the University of Porto (FMUP), Portugal
ST13. LAT1 mRNA derived from extracellular vesicles of colorectal cancer alter the phenotype of recipient cells
Cristina Almeida, Research Center of IPO, Portugal
ST14. Endocytic pathway alterations between multidrug resistant and drug sensitive cells imply different abilities of those cells to release and capture EVs
Cristina P. R. Xavier, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal
ST15. Proteomics Profiling of Extracellular Vesicles enables DLBCL subtyping
Rune Matthiesen, CEDOC, NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Portugal
ST16. Detection of Measurable Residual Disease Biomarkers in Extracellular Vesicles from the Bone Marrow and Peripheral Blood of Multiple Myeloma Patients: a Proof-of-Concept Study
Rui Bergantim, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto; Serviço de Hematologia Clínica do Centro Hospitalar de São João, Portugal
16:25 -16:30 Sponsor talk: Multi parameter analysis of extracellular vesicles using fluorescent nanoparticle tracking analysis (f-NTA) | ONLINE
Sven Kreutel, Sales Director Particle Metrix
16:30 -17:00 Coffee Break
17:00 – 19:00 GENERAL MEETING OF ALL PNEV MEMBERS
20:00 Dinner
DAY 2 | 14th September
9:00 - 12.40 SESSION IV: EVs in TISSUE REGENERATION AND DEGENERATION
Chair: Susana Santos
9:00 – 9:40 Invited talk: Possible roles of extracellular vesicles in the regulation of joint homeostasis
Marca Wauben, Department of Biochemistry and Cell Biology, Utrecht University, The Netherlands
Selected Oral Presentations: EVs for Tissue regeneration
9:40 – 9:55 Selected oral presentation 10: miRNA-modulated extracellular vesicles for Regenerative Medicine applications
Hugo Fernandes, Faculty of Medicine, University Coimbra, Portugal
9:55 – 10:10 Selected oral presentation 11: Temporal profiling of extracellular vesicle (EV) miRNome in hiPSC-cardiomyocyte differentiation for the identification of cell biofactories for therapeutic EV production
Ana Filipa Louro, iBET - Instituto de Biologia Experimental e Tecnológica, Portugal
10:10 – 10:25 Selected oral presentation 12: Circulating Extracellular Vesicles of HFpEF patients induce cellular senescence, endothelial dysfunction and cardiac fibrosis
Elsa D. Silva, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal
Selected oral presentations: EVs in Degenerative Diseases
10:25 – 10:40 Selected oral presentation 13: Unveiling the complexity of extracellular vesicles (EVs) associated with vascular calcification: Heterogeneous populations with different characteristics and features
Carla Viegas, Centre of Marine Sciences (CCMAR), Universidade do Algarve, Portugal
10:40 – 10:55 Selected oral presentation 14: Extracellular vesicle transfer of lncRNA H19 splice variants to cardiac cells
Andreia Vilaça, CNC, University of Coimbra, Portugal; Department of Molecular Genetics, Maastricht University, The Netherlands
10:55 – 11:30 Coffee-break
Chair: Dina Costa Simes
11:30 – 12:10 Invited talk: Studying tetraspanins in EVs
María Yáñez-Mó, CBMSO, Departamento de Biología Molecular, UAM, IIS-IP, Madrid, Spain
12:10 – 13:10 Speed talks presentations
Chair: Dina Costa Simes
ST17. Scalable and chemically-defined manufacturing of mesenchymal stromal cells-derived extracellular vesicles with augmented cardiac regenerative properties
Margarida Costa, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Portugal
ST18. Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation
Alexandra Calle, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, INIA, Spain
ST19. EV-mediated communication between Dendritic Cells and Mesenchymal Stem/Stromal Cells
Filipe Nogueira, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto; ICBAS, Portugal
ST20. Empowering the therapeutic potential of clinically expired platelet concentrates: a new source of regenerative EV.
Ana Meliciano, iBET, Instituto de Biologia Experimental e Tecnológica and ITQB-NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Portugal
ST21. Urinary extracellular vesicles as a tool for Regenerative Medicine
Carlos Jesus, Faculty of Medicine University of Coimbra/Center for Neuroscience and Cell Biology, Portugal
ST22. Exosome-mimetics as potential biotechnological vaccines
Beatriz Benayas, Departamento de Biología Molecular, Universidad Autónoma de Madrid, Spain
ST23. Saliva Exosome Proteins - research on biomarkers for oral and systemic diseases
Tomás Sá, Universidade Católica Portuguesa de Viseu, Faculdade de Medicina Dentária, Portugal
ST24. Use of exosomes-derived mediators as potential biomarkers for cardiovascular diseases: a network approach.
Liliana Moreira-Costa, Department of Surgery and Physiology, Cardiovascular Center, Faculty of Medicine of University of Porto, Portugal
ST25. Extracellular vesicles: a promising delivery system for silencing sequences to treat Machado-Joseph Disease
David Rufino-Ramos, CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Portugal
ST26. The extracellular vesicles in the left-right organizer of zebrafish
Sara Pestana, CEDOC, NOVA Medical School / Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Portugal
ST27. Re-establishment of miR-146a levels in ALS cortical astrocytes prevents inflammatory dissemination by modulation of inflamma-miRNA profile in cells and small extracellular vesicles
Gonçalo Garcia, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, Portugal
ST28. Neuronal miR-124 regulates microglia polarization by secretome-mediated paracrine signaling
Gonçalo Garcia, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon, Portugal
ST29. Extracellular vesicles-mediated delivery of CRISPR/Cas9 tools to treat Machado-Joseph Disease
Kevin Leandro, Center for Neuroscience and Cell Biology, Portugal
ST30. Extracellular communication mediated by EVs in a mouse brain
David Rufino-Ramos, Center for Neuroscience and Cell Biology, University of Coimbra, Portugal
13:10 – 13:20 FUTURE PERSPECTIVES, PRIZES FOR BEST PRESENTATIONS AND CLOSING SESSION
13:20 – Take away lunch
Abstract Submission
Abstract submission deadline: 5th July 2021
Notification of acceptance will be given by the 12th of July
Abstracts will be selected for oral presentation, or for poster (which may be replaced by speed talk presentation, according to the pandemic situation).
Registration
The meeting will be held at Mariano Gago Auditorium (i3S) with video transmission to the Corino de Andrade Auditorium if necessary.
The event is limited to 85 participants, in order to ensure the personal hygiene' rules and an adequate social contact conduct. AT THE MOMENT THIS NUMBER IS NEARLY REACHED AND FOR THAT REASON WE CAN NOW ONLY ACCEPT REGISTRATION FROM PRESENTING AUTHORS AND MEMBERS FROM THE SCIENTIFIC COMMITTEE. If the pandemic context allows more participants, we will reopen registrations later.
If you wish to stay in the waiting list, please send an e-mail to events@i3s.up.pt.
Fees:
The registration fee includes coffee-breaks and a lunch box.
|
Before 23rd July 2021 |
Until the 1st of September 2021 |
|
| Students * | 60€ | 100 € |
| Other participants | 120€ | 240€ |
| Dinner (optional) | 40€ | -------- |
*Proof of student registration in the academic year 2020/2021 must be uploaded during registration.
More information:
Events Management Unit | Rua Alfredo Allen 208 | 4200-135 Porto, Portugal
Email: events@i3s.up.pt | Tel: +351 226 074 900
Sponsors