Scientific Platforms

One of the major advantages of modern life and health sciences research are the scientific platforms armed with cutting-edge technologies. Given the important role scientific platforms play in a research facility's day-to-day, i3S devotes much effort to its own platforms and considers them to be a top priority. 

Our platforms provide expert support and access to a range of specialized equipment. Platforms are coordinated by researchers and managed by highly qualified and specialized technicians. They have been valuable enhancer hubs for the establishment of synergies and networking activities. 

01. ANIMAL FACILITY

The animal facility is a scientific platform that allows the use of SPF mice, rats and rabbits. It supports the development of animal models of disease by the use of transgenic, oncological, surgical and infection models (level 2 and 3). In vivo imaging technologies (ultrasound, x-ray, bioluminescence) are also supported by the facility. This facility shares and reinforces the application of the 3Rs and the improvement of animal welfare as key points for the use of animals.

TEAM

Sofia Lamas (Technical Coordinator) | Mónica Sousa (Scientific Coordinator) | Marlene Morgado | Filipa Franquinho Ferreira | Ângela Ribeiro | Fátima Moreira | Julieta Rodrigues | Isabel Duarte | Liliana Silva | Lígia Martins | Maximilian Alves | Patrícia Moreira | Amândio Costa | João Guimarães | Mercês Abreu | Nadiya Malchenko

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02. BIOINTERFACES AND NANOTECHNOLOGY

The Biointerfaces and Nanotechnology (BN) core facility provides services and technical training for i3S, as well as for other research centres and industry, in the areas of interfaces and macromolecules. BN includes advanced physical, chemical and structural characterisation techniques particularly dedicated to the surface study of biomaterials, cells and tissues, going down to the micrometric and nanometric level.

TEAM:

Manuela Brás (Technical Coordinator) | Pedro Granja (Scientific Coordinator) | Ricardo Vidal (Technician)

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03. b. IMAGE – BIOIMAGING CENTRE

The aim of b.IMAGE is to advance in the development, improvement, integration and use of bioimaging solutions through research, technology development and education. It provides high quality in vitro and in vivo imaging equipment for the biological, biomedical and bioengineering community, supporting users in every stage of their experiment, from experimental design and data acquisition to analysis. Our equipment includes: confocal Raman microscope (LabRAM HR800 UV, Horiba Jobin-Yvon); imaging flow cytometer (ImageStreamX, Amnis, EDM Millipore); atomic force microscope coupled to an inverted fluorescence microscope (PicoPlus scanning probe microscope, Agilent Technologies); spectral confocal microscope (TCS-SP5 AOBS; Leica); and in vivo micro-ultrasounds imaging system (Vevo 2100, Visualsonics).

For more information please visit www.bimage.ineb.up.pt.

TEAM:

Ana Paula Pêgo (Scientific Coordinator) | María Gómez Lázaro (Technical Coordinator | responsible for in vitro imaging) | Diana Nascimento (responsible for in vivo imaging) | Manuela Brás (responsible for Atomic Force Microscopy) | Dalila Fernanda Neto Pedro (responsible for Fluorescence Microscopy) | Cristina Ribeiro (Confocal Raman Microscopy, Expert in Confocal RAMAN) | Maria Jose Oliveira (Expert in Confocal Microscopy) | Paulo de Castro Aguiar (Expert in Image Analysis, Mathematical Modeling & Computational Biology)

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04. X-RAY CRYSTALLOGRAPHY PLATFORM

The X-ray Crystallography Platform allows high-resolution structural characterisation of small molecules, macromolecules, and macromolecular complexes. The experimental framework of the resulting atomic models provides valuable insight into the mode of action of the molecules of interest. At the core of the Platform is a a 4-circle, dual wavelength (Cu and Mo) single-crystal X-ray diffractometer equipped with a cryostat and a large area CCD detector for data collection from both macromolecular and small molecule samples.

TEAM:

Pedro Pereira (Scientific Coordinator)

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05. B2Tech - BIOCHEMICAL AND BIOPHYSICAL TECHNOLOGIES

B2Tech provides scientific support and access to state-of-the-art technologies in the fields of production of recombinant proteins and of biochemical/biophysical analysis of biomolecules, focusing its work on three major fields of expertise: 

I. recombinant protein expression and purification;

II. structural and functional analysis of biomolecules and their stability;

III. characterisation of molecular interactions.

The research facility has an open-access policy and the resources are available to all scientific community and industry.

For information about access to available resources, please contact Frederico Silva. For general information, have a look into the platform's website.

TEAM:

Frederico Silva (Technical Coordinator) | Sandra Macedo Ribeiro (Scientific Coordinator) 

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06. PCF - PROTEOMICS CORE FACILITY

The Proteomics Core Facility (PCF) provides mass spectrometry-based approaches for the identification and characterisation of proteins from biological and biomedical backgrounds. We have expertise in the design and implementation of tailored proteomics methodologies for experimental procedures, research grants, data analysis, and scientific publications. The PCF participates in graduation programs, organises workshops and continuously innovates on technologies and protocols.

PCF is a node of the Portuguese Mass Spectrometry Network  - RNEM

TEAM:

Hugo Osório (Technical Coordinator) | Celso Reis (Scientific Coordinator)

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07. BIOSCIENCES SCREENING UNIT

The mission of the BioSciences Screening Unit is to provide researchers with the ability to run genetic and chemical screens for the purpose of target and/or drug discovery. This unit's main focus is on assay development, high-throughput screening, high-content screening, multiplexing technologies and data analysis.

TEAM:

André Maia (Technical Coordinator) | António Pombinho (Scientific Coordinator)

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08. TRACY – TRANSLATIONAL CYTOMETRY

The Translational Cytometry platform is equipped with flow cytometers (sorter and analysers) and a workstation for data analysis; it also provides education for a proper use of the equipment. Multi-parameter and high content cellular analyses, qualitative and quantitative evaluation and detection of rare cells are some of the features that characterise this technology, with applications in many different research areas.

TEAM:

Catarina Meireles (Technical Coordinator) | Nuno Alves (Scientific Coordinator) | Emília Cardoso (Technician)

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09. CCGEN – CELL CULTURE AND GENOTYPING SERVICE

The Cell Culture and Genotyping Unit (CCGen) aims at facilitating the implementation of state-of-the-art advances in cell culture, genotyping and gene expression technology, by providing i3S researchers with selected services, expert consultation and training in cell culture, genotyping and gene expression techniques.

TEAM:

Paula Magalhães (Technical Coordinator) | Anabela Cordeiro-da-Silva (Scientific Coordinator) | Tânia Meireles (Technician) 

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10. IN VIVO CAM ASSAYS UNIT

The In vivo CAM assays unit is a core facility that provides scientific expertise and services using the chick embryo model specifically, its chorioallantoic membrane (CAM). This research facility provides access to alternative/additional in vivo tools to evaluate angiogenesis, tumorigenesis, invasion, metastization and vascular permeability. The available collection of functional assays can be applied to cells, biomaterials, drugs, supernatants or extracts. The facility is fully equipped to address new challenges and widen model applications.

TEAM:

Marta Teixeira Pinto (Technical Coordinator) | Joana Paredes (Scientific Coordinator)

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11. GENCORE – GENOMICS CORE FACILITY

The Genomics Core Facility - GenCore is a full service facility dedicated to provide investigators state of the art technological solutions in the field of genomics and high throughput analysis. We offer technical expertise and support with experimental design, protocol development, and data analysis guidance. Training is another crucial aspect of our work. The laboratory is proficient in providing: Sanger/NGS sequencing, exome sequencing, targeted DNA and RNA sequencing, small genome sequencing, metagenome sequencing, whole transcriptome sequencing, real-time PCR and digital PCR. Additionally, due to the very dynamic and rapidly evolving field of genomics, we actively collaborate with different companies in developing NGS related products both for sample preparation and for bioinformatics data analysis. The facility is an Ion Torrent certified service provider.

TEAM:

Mafalda Rocha (Technical Coordinator) | José Luís Costa (Scientific Coordinator) | Rob Mensink 

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12. ALM – ADVANCED LIGHT MICROSCOPY UNIT 

The Advanced Light Microscopy unit (ALM) is a scientific core facility of I3s dedicated to state-of the art optical microscopy applied to biosciences research. The ALM gives open access to high-end instruments for advanced fluorescence microscopy, live cell imaging and image data analysisThe ALM team provides scientific advice in experiment planning, training in hands-on operation of the equipment and in image data analysis, as well as, collaborates in the development of research projects.

The ALM annually organizes post-graduation courses in the field and participates in master and doctoral programs, plus outreaching activities to high schools.

ALM is a node of the PPBI-Portuguese Platform of BioImaging and member of the ELMI-European Light Microscopy Initiative.

PPBI - http://www.ppbi.pt | ELMI - http://www.embl.org/elmi/

TEAM:

Paula Sampaio (Technical Coordinator) | Patricia Castro (Microscopy Specialist) | Claudio Sunkel (Scientific Coordinator)

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13. HEMS – Histology and Electron Microscopy Service

HEMS is a core facility open to researchers from the i3S as well as from other institutes. The Facility provides services and supervision related to Electronic Microscopy (EM), and Optical Microscopy (OM) with the ancillary equipment. The Facility provides both the equipment and technical support to researchers needing high level optical and EM to tackle studies either of biological samples or material sciences. It also offers training courses as well as guided visits for high school students in collaboration with the Communication Unit.

PPBI - http://www.ppbi.pt

TEAM:

Rui Fernandes (Technical Coordinator) | João Relvas (Scientific Coordinator - HEMS) | Paula Soares (Scientific Coordinator - Histopathology) | Rossana Correia (Principal Technician - Histopathology) | Francisco Figueiredo (Technician) | Nuno Mendes (Technician) | Roberto Salema (Scientific Consultant - HEMS)

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