Organoids systems for the study of infection, 1^st edition

5-9 July 2021 | i3S – Instituto de Investigação e Inovação em Saúde

Host-pathogen interactions can be studied using a great variety of models, either simple or complex. Organoids systems are currently being developed for infection studies, offering a 3D microenvironment that can mimic the complexity of host-immune interactions, and that at the same time, modelling human-specific pathogenesis and exploring infection-associated carcinogenesis.

This workshop will explore organoids as models for infection, with one day of theoretical classes covering organoids methods for infection using bacteria and virus, and hands-on classes where students will learn different techniques to infect organoids.

Target audience: MSc and PhD Students, Early Stage Researchers

Course approximate duration: 16 hours

Requirements:

• Participants coming from outside Portugal should follow the measures implemented by the Portuguese government for entering the country, at the date of the travel, taking into account their country of departure/origin.
• Labcoat for practical classes.
• Laptop for the theoretical classes.

Course organizers: Anna Olsson; Marta Alves da Silva, Margarida Saraiva, Sina Bartfeld, Carmen Aguilar.

Poster >>

The course will have 8 hours of theoretical lessons covering the following issues:

1. Organoids models for infectious diseases
2. Methods for organoids infection
3. Screening and getting data out of the host-pathogen model
4. Gastric organoids infection

The practical classes will be divided between Wuerzburg and Porto, with Sina Bartfeld and Carmen Aguilar showing infection methods using human gastric organoids, and Marta Alves da Silva teaching other infection methods using murine material.

Hands-on training will consist in showing how to establish gastric organoids (1st part), and then, the infection method using bacteria, such as E.coli.
Practical classes will consist of two sessions:
- Isolation of gastric organoids, where students will learn how to establish these cultures.
- Infection methods using gastric organoids, teaching two infection methods (monolayer and microinjection, for example).

Andreas Hocke, Charité University, Berlin, Germany

Mirjana Kessler, LMU Klinikum, Muniche, Germany

Carmen Aguilar, University of Würzburg, Germany

Sina Bartfeld, University of Würzburg, Germany

Anna Olsson, i3S, Portugal

Margarida Saraiva, i3S, Portugal

Marta Alves da Silva, i3S, Portugal
 

Registration fee includes workshop participation and certificate.

Fees:

i3S and UP members – 50€

Others – 75€

Participants will be selected and priority will be given to those who fulfil the following requirements:

- Degree in Biology, Bioengineering or similar areas.

- PhD student/ early-stage researcher in these areas.

- Experience in cell culture.

Considering your work, please tell us what is your interest in the workshop. Are you planning to use organoids models of infection in the near future?

Pre-registration form >>

Pre-registration deadline: 24^th May 2021

Payment will be requested only after confirmation of acceptance.

The organizer reserves the right to cancel the course in case of an insufficient number of participants or other unforeseeable events that render the execution of the course.

This course is organized in collaboration with the REMODEL project which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no 7857491.

More Information
Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 220 408 800