Where Ideas Grow

Flow Cytometry Workshop
  
From basic knowledge to advanced applications

07th-10th October 2019 | i3S - Instituto de Investigação e Inovação em Saúde

 

This workshop intends to provide a firm understanding of the principles and applications of Flow Cytometry, combining the theoretical and practical sessions. Applicants may register in basic theoretical training modules only, or select the complete training including theoretical and practical sessions.

 

Course Contents:
• Principles of Flow Cytometry
• Fluorophores and Panel design
• How to perform a Multi-color experiment
• Perform compensations and analyze data
• Required controls and statistical analysis for data publication
• Applications: Immunophenotyping, cell cycle, apoptosis, phospho and soluble proteins by flow cytometry.
• Flow cytometry analysis with FlowJo Software (Licenses included)

 

Practical topics:
• Flow cytometry analysis with FlowJo Software (Licenses included)
• Design a multi-colour panel

 

Target Audience:
Scientists (researchers, students, medical doctors, technicians, engineers) with notions of Flow Cytometry.

 

Sobre

Flow Cytometry Workshop
  
From basic knowledge to advanced applications

07th-10th October 2019 | i3S - Instituto de Investigação e Inovação em Saúde

 

This workshop intends to provide a firm understanding of the principles and applications of Flow Cytometry, combining the theoretical and practical sessions. Applicants may register in basic theoretical training modules only, or select the complete training including theoretical and practical sessions.

 

Course Contents:
• Principles of Flow Cytometry
• Fluorophores and Panel design
• How to perform a Multi-color experiment
• Perform compensations and analyze data
• Required controls and statistical analysis for data publication
• Applications: Immunophenotyping, cell cycle, apoptosis, phospho and soluble proteins by flow cytometry.
• Flow cytometry analysis with FlowJo Software (Licenses included)

 

Practical topics:
• Flow cytometry analysis with FlowJo Software (Licenses included)
• Design a multi-colour panel

 

Target Audience:
Scientists (researchers, students, medical doctors, technicians, engineers) with notions of Flow Cytometry.

 

Program

Preliminary Program

  BASIC ADVANCED
  DAY 1 DAY 2 DAY 3 DAY 4
09:30-10:40 Principles of Flow Cytmetry Immunophenotyping Cell Death & Apoptosis Introduction to
FlowJo Software
10:45-11:00 Coffee Break Coffee Break Coffee Break Coffee Break
11:00-12:00 Publishing Flow Data-Part 1 Multicolor Experiment
Panel Design-1
Cell Cycle & Proliferation Hands-on FlowJo
Software
12:00-13:30 Lunch Lunch Lunch Lunch
13:30-15:00 Fluorochromes, Brightness
and Detection
Multicolor Experiment
Panel Design-2
Cell Sorting Flow Statistical
Analysis
15:00-15:15 Coffee Break Coffee Break Coffee Break Coffee Break
15:15-17:00 Applications (Cell culture,
Apoptosis, cell cycle)
Circulating Tumor Cells (CTCs) Publishing Robust Flow
Data-Part 2
FlowJo Tips and Tricks
17:00-17:30 Discussion Discussion Discussion Discussion

 

Programa

Preliminary Program

  BASIC ADVANCED
  DAY 1 DAY 2 DAY 3 DAY 4
09:30-10:40 Principles of Flow Cytmetry Immunophenotyping Cell Death & Apoptosis Introduction to
FlowJo Software
10:45-11:00 Coffee Break Coffee Break Coffee Break Coffee Break
11:00-12:00 Publishing Flow Data-Part 1 Multicolor Experiment
Panel Design-1
Cell Cycle & Proliferation Hands-on FlowJo
Software
12:00-13:30 Lunch Lunch Lunch Lunch
13:30-15:00 Fluorochromes, Brightness
and Detection
Multicolor Experiment
Panel Design-2
Cell Sorting Flow Statistical
Analysis
15:00-15:15 Coffee Break Coffee Break Coffee Break Coffee Break
15:15-17:00 Applications (Cell culture,
Apoptosis, cell cycle)
Circulating Tumor Cells (CTCs) Publishing Robust Flow
Data-Part 2
FlowJo Tips and Tricks
17:00-17:30 Discussion Discussion Discussion Discussion

 

Speakers

Tim Bushnell

University of Rochester Medical Center

 

Serena Di Cecilia
FlowJo

 

Alexandre Salvador
Enzifarma

 

José Rino

IMM

 

Carlos Palmeira

IPO

 

Catarina Meireles

i3S

Oradores

Tim Bushnell

University of Rochester Medical Center

 

Serena Di Cecilia
FlowJo

 

Alexandre Salvador
Enzifarma

 

José Rino

IMM

 

Carlos Palmeira

IPO

 

Catarina Meireles

i3S

Registration

Registration includes all course materials and refreshments. Lunches are not included in the registration fees.
Limited number of participants.

Fee:

Registration types Early Bird Registration
Until June 30th 2019
Late Registration
From June 30th to September 13th 2019
  Basic
(Day 1-2)
Basic + Advanced
(Day 1-4)
Basic
(Day 1-2)
Basic + Advanced
(Day 1-4)
i3S members / PhD students* 120€ 180€ 150€ 210€
UP members and other
Research institutions
150€ 225€ 180€ 255€
Industries/companies 200€ 300€ 250€ 350€

 

* Students should enclose a document confirming status.

Full course (Basic + Advanced - Day 1-4) is full. If you wish to stay in the waiting list, please send an e-mail to events@i3s.up.pt. You will be contacted if there are any cancellation. 

Inscrição

Registration includes all course materials and refreshments. Lunches are not included in the registration fees.
Limited number of participants.

Fee:

Registration types Early Bird Registration
Until June 30th 2019
Late Registration
From June 30th to September 13th 2019
  Basic
(Day 1-2)
Basic + Advanced
(Day 1-4)
Basic
(Day 1-2)
Basic + Advanced
(Day 1-4)
i3S members / PhD students* 120€ 180€ 150€ 210€
UP members and other
Research institutions
150€ 225€ 180€ 255€
Industries/companies 200€ 300€ 250€ 350€

 

* Students should enclose a document confirming status.

Full course (Basic + Advanced - Day 1-4) is full. If you wish to stay in the waiting list, please send an e-mail to events@i3s.up.pt. You will be contacted if there are any cancellation. 

Sponsors

More Information:
Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 220 408 800

 

Patrocinadores

More Information:
Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 220 408 800