Flow Cytometry Workshop

From basic knowledge to advanced applications

07th-10th October 2019 | i3S - Instituto de Investigação e Inovação em Saúde

This workshop intends to provide a firm understanding of the principles and applications of Flow Cytometry, combining the theoretical and practical sessions. Applicants may register in basic theoretical training modules only, or select the complete training including theoretical and practical sessions.

Course Contents:
• Principles of Flow Cytometry
• Fluorophores and Panel design
• How to perform a Multi-color experiment
• Perform compensations and analyze data
• Required controls and statistical analysis for data publication
• Applications: Immunophenotyping, cell cycle, apoptosis, phospho and soluble proteins by flow cytometry.
• Flow cytometry analysis with FlowJo Software (Licenses included)

Practical topics:
• Flow cytometry analysis with FlowJo Software (Licenses included)
• Design a multi-colour panel

Target Audience:
Scientists (researchers, students, medical doctors, technicians, engineers) with notions of Flow Cytometry.

Poster download

Program

Preliminary Program

	BASIC		ADVANCED
	DAY 1	DAY 2	DAY 3	DAY 4
09:30-10:40	Principles of Flow Cytmetry	Immunophenotyping	Cell Death & Apoptosis	Introduction to FlowJo Software
10:45-11:00	Coffee Break	Coffee Break	Coffee Break	Coffee Break
11:00-12:00	Publishing Flow Data-Part 1	Multicolor Experiment Panel Design-1	Cell Cycle & Proliferation	Hands-on FlowJo Software
12:00-13:30	Lunch	Lunch	Lunch	Lunch
13:30-15:00	Fluorochromes, Brightness and Detection	Multicolor Experiment Panel Design-2	Cell Sorting	Flow Statistical Analysis
15:00-15:15	Coffee Break	Coffee Break	Coffee Break	Coffee Break
15:15-17:00	Applications (Cell culture, Apoptosis, cell cycle)	Circulating Tumor Cells (CTCs)	Publishing Robust Flow Data-Part 2	FlowJo Tips and Tricks
17:00-17:30	Discussion	Discussion	Discussion	Discussion

Speakers

Tim Bushnell

University of Rochester Medical Center

Serena Di Cecilia
FlowJo

Alexandre Salvador
Enzifarma

José Rino

IMM

Carlos Palmeira

IPO

Catarina Meireles

i3S

Registration

Registration includes all course materials and refreshments. Lunches are not included in the registration fees.
Limited number of participants.

Fee:

Registration types	Early Bird Registration Until June 30^th 2019		Late Registration From June 30^th to September 13^th 2019
	Basic (Day 1-2)	Basic + Advanced (Day 1-4)	Basic (Day 1-2)	Basic + Advanced (Day 1-4)
i3S members / PhD students*	120€	180€	150€	210€
UP members and other Research institutions	150€	225€	180€	255€
Industries/companies	200€	300€	250€	350€

* Students should enclose a document confirming status.

Full course (Basic + Advanced - Day 1-4) is full. If you wish to stay in the waiting list, please send an e-mail to events@i3s.up.pt. You will be contacted if there are any cancellation.

Registration form

Program

Speakers

Registration

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