Where Ideas grow

Interrogations at the Biointerface | 7th Advanced Summer School
Do organoids fill the in vivo / in vitro gap?


27-28 June 2019

Biblioteca Almeida Garrett, Porto


In 2019, the “Interrogations at the Biointerface - 7th Advanced Summer School” with the specific topic “Do organoids fill the in vitro/in vivo gap?” will take place in Porto, organized by i3S (Instituto de Investigação e Inovação em Saúde) / INEB (Instituto de Engenharia Biomédica) of the University of Porto.

During 10 years in a row (1999-2009), INEB has organized Summer Courses with lectures and hands-on lab sessions - the Advanced Summer Course in Cell/Material Interactions. After a short break in 2010, the Summer Schools re-emerged under the general topic of Interrogations at the Biointerface with the goal of bringing together different fields around one topic of interest.

This year edition of the Advanced Summer School will be held in Biblioteca Almeida Garret, in the beautiful city of Porto from the 27th to 28th of June, 2019. These two days will be devoted to lectures around an innovative topic – Organoids - with renowned and expert speakers. Participants are encouraged to submit their research work to be present either an oral presentation or poster. The lectures will take place in a very informal environment and participants are encouraged to participate and bring questions that can contribute to the advance of knowledge through interactive approaches.

The venue is in a library located at the gardens of Palácio de Cristal, a magic environment from the Romantic period in the late XIX century, just on the top of the city with an amazing view to the Douro river. We are sure that this atmosphere will be memorable and we believe that this course will be most valuable.


We are looking forward to welcoming you at the 7th Advanced Summer School!


Several cutting-edge topics will be explored during the course:

* Complex 3D culture systems
* Sophisticated tissues in a dish
* Design biomaterials as extracellular matrix mimics for 3D cell culture
* Organoids to mimic development and disease
* Culture and analysis of organoids
* Ethical implications of organoids
* The consciousness of cerebral organoids


Biblioteca Municipal Almeida Garrett (Almeida Garrett Library)
R. de Entre-Quintas 268, 4050-344 Porto


The Almeida Garrett Library is located in the Gardens of Palácio de Cristal, a delightful green space with some of the best panoramic views of the Douro river. These romantic gardens from the XIX century are surrounded by several iconic spaces of Porto’s romantic period such as the Romantic Museum, the Macieirinha Villa, the Tait Villa and the Porto Wine Mansion.

The Library building includes a modern auditorium with a capacity to hold 192 people and a gallery with a program of regular expositions covering various themes, including arts, cultural heritage and science.

Its privileged location, near green space in the heart of the city, and with several spaces, makes it a centre of important local activities, including theatre, cinemas, conferences, poetry sessions or musical spectacles.





Ana Paula Pêgo
Cristina Barrias
Judite Novais Barbosa
Mário Barbosa
Sílvia Bidarra
Sofia Duque Santos


27th  June 2019


08:45-09:15 Registration

09:15-09:30 Welcome


09:30-10:30 Engineering morphogenesis through instructed self-organization

Cristina Barrias; Bioengineered 3D Microenvironments Group; i3S/ INEB; Portugal

Chair: Paula Parreira 


10:30-11:00 Coffee break

11:00-12:00 Kidney tubuloids for drug testing

Roos Masereeuw; Department of Pharmaceutical Sciences; Utrecht University; The Netherlands

Chair: Sofia Santos 


12:00-13:00 Selected oral communications | Chairs: Daniela Vasconcelos & Ana Catarina Pereira

A role for MSCs secretome to modulate the inflammatory response in disc degeneration
Joana R. Ferreira, i3S, INEB and ICBAS, Portugal

High-throughput cell-in-gel microarray platform for the analysis of cell behaviour in 3D matrices
Pedro Alves, i3S, INEB, Portugal 

Response of epithelial mammary cells to changes in matrix stiffness in mechanically-tunable hydrogel-based 3D models
Sílvia Bidarra, i3S, INEB, Portugal 

Design of photoclickable bioinks for 3D bioprinting of tissue models 
Rúben Pereira, i3S, INEB, Portugal 


13:00-14:00 Lunch

14:00-15:00 Infection, innate immune signaling and cancer in the gut - organoids as model

Sina Bartfeld; Institute for Molecular Infection Biology; University of Würzburg; Germany

Chair: Sílvia Bidarra


15:00-16:30 Selected oral communications | Chairs: Flávia Sousa & Rute Nunes 

Bioengineering Spherical 3D In vitro Tumor Models Through ECM-mimetic Platforms 
Vítor Gaspar, CICECO – Aveiro Institute of Materials, University of Aveiro, Portugal

Pancreatic cancer patient-derived 3D organotypic cultures as models for preclinical analysis
Naomi Walsh, National Institute for Cellular Biotechnology, Dublin City University, Ireland

The role of NLRP3 inflammasome in the inflammatory response to 3D chitosan scaffolds 
Daniela P. Vasconcelos, i3S, INEB and ICBAS, Portugal

The systemic immune response to collagen-induced arthritis and the impact of bone injury in inflammatory conditions 
José H. Teixeira, i3S, INEB and ICBAS, Portugal 

3D fibrinogen scaffolds support Osteoclastogenesis: impact on scaffold degradation and mesenchymal stem/stromal cell differentiation
Mafalda Gonçalves, i3S, INEB and ICBAS, Portugal

Establishment of human model of intervertebral disc degeneration and associated immune response 
João V. Côrte-Real, i3S, INEB; FFUP and ICBAS, Portugal 


16:30-17:00 Coffee break


17:00-18:00 Photoadaptable hydrogels for studying and manipulating crypt formation in intestinal organoids

Kristi Anseth; Anseth Research Group, University of Colorado; USA

Chair: Mário Barbosa


18:00 i3S Choir and Port Wine Tasting 


20:00 Summer School Dinner


28th  June 2019


09:00-10:00 Organ Bioengineering: The Future of Transplantation Medicine

Pedro Batista; Centro de Investigación Biomédica Aragón; Spain

Chair: Cristina Barrias


10:00-11:00 Bioengineering Neural Tube Morphogenesis

Adrian Ranga; Laboratory of Bioengineering and Morphogenesis; Belgium

Chair: Maria Inês Almeida


11:00-11:30 Coffee break


11:30-12:30 Poster Session


12:30-13:15 Selected oral communications | Chair: Estrela Neto 

Engineering intestinal mucosa cells interactions in a 3D in vitro model 
Joana Costa, Research Center “E. Piaggio” of the University of Pisa, Italy

3D bioengineered intestine for drug screening absorption studies
Maria Helena Macedo, i3S, INEB and ICBAS, Portugal 

New signalling pathways for intestinal stem cell identity in development and disease
Bruno Pereira, i3S, IPATIMUP, Portugal 


13:15-14:10 Lunch


14:00-14:15 3D bioprinting: the future of medicine is here!

Matteo Fiammetta, IZASA 


14:15-15:00 Selected oral communications | Chair: Rúben Pereira

Clarifying interplay between dental MSC and biomimetic material with real-time in vivo imaging model 
Christiane Laranjo Salgado, i3S, INEB, Portugal 

Lipid nanoparticles biocompatibility: do in vitro and in vivo studies reveal the same info?
Joana Magalhães, LAQV, REQUIMTE, FFUP and ICBAS, Portugal 

Fetal extracellular matrix of nucleus pulposus as a natural scaffold for intervertebral disc rejuvenation
Morena Fiordalisi, i3S, INEB and ICBAS, Portugal 


15:00-16:00 Ethical issues concerning human organoids

Andrea Lavazza; Centro Universitario Internazionale Arezzo; Italy

Chair: Ana Paula Pêgo


16:00-16:30 Coffee break


16:30-17:30 Round Table | Moderator: Mário Barbosa, i3S, INEB, Portugal 

José Carlos Bessa, i3S, IBMC, Portugal 
Anna Olsson, i3S, IBMC, Portugal 
Andrea Lavazza, Centro Universitario Internazionale Arezzo, Italy
Cristina Barrias, i3S, INEB, Portugal 


17:30-18:00 Closing session


Adrian Ranga

Laboratory of Bioengineering and Morphogenesis, Belgium

Adrian Ranga is the head of the Laboratory of Bioengineering and Morphogenesis in the Biomechanics Division of the Department of Mechanical Engineering at KU Leuven. He was trained as a mechanical engineer at McGill University and subsequently completed his PhD studies at EPFL on the development of systems-level bioengineering platforms to understand stem-cell niche interactions.  Adrian Ranga was then awarded an SNF post-doctoral fellowship to study the development of the human muskoloskeletal system using stem-cell derived vitro models at Harvard Medical School. The focus of his research group is on developing novel bioengineering approaches to study the response of stem cells to their microenvironment, with a particular interest in how combinations of mechanical forces and biochemical cues shape multicellular morphogenesis.   



Andrea Lavazza

Centro Universitario Internazionale Arezzo, Italy

Andrea Lavazza, a scholar of cognitive sciences and a philosopher, is a research fellow at Centro Universitario Internazionale, Arezzo, Italy. His main field of research is neuroethics, which he has helped to introduce in Italy. In this field he has published mainly papers on enhancement and memory manipulation. His interests are focused on moral philosophy, free will, and law at the intersection with cognitive sciences. He is working on naturalism and its relations with other kinds of causation and explanation in philosophy of mind and philosophical anthropology. Lavazza is the author or the editor of eleven books.



Cristina Barrias

Bioengineered 3D Microenvironments Group, i3S/ INEB, Portugal

Cristina Barrias is the head of the Bioengineered 3D Microenvironments group at i3S-Instituto de Investigação e Inovação em Saúde, University of Porto. She graduated in Chemical Engineering at the Faculty of Engineering of the University of Porto, and got her PhD in Engineering Sciences, in the field of Biomaterials & Regenerative Medicine (UP). Her PhD studies were performed at INEB-Instituto de Engenharia Biomédica and at IBMC-Instituto de Biologia Molecular e Celular, and she was also a visiting researcher at INSERM U443, University of Bordeaux, France. She carried out her postdoc at INEB/IBMC in collaboration with the University of Nevada-Reno, USA. She is currently Principal Investigator at i3S/INEB and invited Associate Professor at Instituto de Ciências Biomédicas Abel Salazar (ICBAS, UP). The focus of her group is on developing bioengineered microenvironments to promote controlled 3D cell assembly into complex microtissues. These microtissues are being explored as building blocks for stem cell-based regenerative therapies and tissue engineering, and also as advanced 3D models for in vitro studies.




Kristi Anseth

Anseth Research Group, University of Colorado, USA

Kristi Anseth is a world-class researcher in biomaterials and regenerative medicine. She engages molecular biology, materials engineering, and biochemistry in the development of synthetic extracellular matrices that mimic physiological environments. Her work is informed by a background in chemical engineering; she received a B.S. in chemical engineering from Purdue University and a Ph.D. in chemical engineering from the University of Colorado Boulder. Following this, she completed postdoctoral work at Purdue and MIT. At MIT, she worked under the mentorship of Robert Langer, a leader in the field of biomedical engineering. Today, Langer calls Anseth “one of the top bioengineers in the world”. Once an exceptional student, Kristi Anseth is now at the forefront of her field, using a materials-first approach to address major hurdles in regenerative medicine. Anseth’s group works at the interface of biology and engineering, as her focus on essential cellular functions translates to biomaterials design.



Pedro Batista

Centro de Investigación Biomédica Aragón, Spain

Pedro Baptista is originally from Portugal, where he graduated from the School of Pharmacy of the University of Lisbon. After that, he worked on clinical research at Eli Lilly & Co before engaging in his doctoral dissertation work, after being selected to the prestigious Gulbenkian PhD Program in Biomedicine. From his PhD thesis project, resulted the generation of the first human liver ever made in a laboratory, impacting the scientific community at large and widely broadcasted in the media all around the world. He is currently a Group Leader at the Health Research Institute of Aragon (IIS Aragon) in Zaragoza, Spain and the founder of the Organ Bioengineering and Regenerative Medicine Laboratory at this institution. He is also an Assistant Professor at the Department of Biomedical and Aerospace Engineering at University Carlos III of Madrid, Spain. His current research focuses on creating bioengineered livers that can finally make the long-term transplantation of these lab-grown organs a reality. He is also working on liver stem cell biology and the development of novel methods to expand fetal and adult human stem/progenitor cells to the required large numbers necessary for organ bioengineering. Pedro Baptista is also interested in applying bioengineered hepatic tissues and organs to study developmental biology, physiology and drug discovery. 



Sina Bartfeld

Institute for Molecular Infection Biology, University of Würzburg, Germany

Sina Bartfeld is currently group leader at the Research Center for Infecious Diseases, University of Wurzburg, Germany. Her main research areas are organoids and gastric infection. The Bartfeld lab is interested in host-microbe interactions at the epithelial lining of the gastrointestinal tract. Here, epithelial cells function as a physical and immunological barrier between the microbes of the gut and the body. To model the interactions, gastrointestinal organoids are used. They are generated from adult epithelial stem cells from patient samples or from laboratory animals. Organoids are 3-dimensional mini-versions of the original organ. They contain stem cells as well as differentiated cells, which organize themselves around a central lumen. 



Roos Masereeuw

Department of Pharmaceutical Sciences, Utrecht University, The Netherlands

Roos Masereeuw is an expert on developing humanized in vitro systems that functionally mimic (patients) organs, which includes the use of innovative technologies for 3-dimensional organ cultures such as microfluidics (organs-on-chip technology). These experimental tools should aid in translating molecular interactions into therapeutic effects. Based on the unique, patented, human renal cell lines with a high predictive value for drug and waste product transport and metabolism, her team is developing a bioartificial kidney, a kidney-on-a-chip device suitable for in vitro toxicity testing and research into the renal tubular secretion and reabsorption machinery. She holds the chair in Experimental Pharmacology at the University of Utrecht.

Abstract Submission

We encourage all participants to submit an abstract with their most recent data, to be presented at the course. We expect to stimulate lively discussions and exchange of ideas between participants and speakers.

The abstract will be evaluated and selected either for oral or poster presentation. A prize will be given to the best poster and oral presentation being presented at this Summer School.


The deadline for normal abstract submission will be 15th May 2019 and author notifications will be sent until the 20th  May 2019.


Last Minute Abstracts for Poster Presentation from 21th May to 10th June


The authors should follow the layout format of the template available on the web site. The template is prepared in MS-word and can be downloaded:


Please submit your abstract:


The registration fee includes coffee breaks, lunches and the course material.  


Registration until 17th June 2019 | Payment deadline: 17th June 2019

Students* – 85€

i3S members – 145€

Other participants – 185€


Social event registration fees:

Summer School Dinner: 25 €


* Bachelor´s, Master´s, PhD’s Students

Students should enclose a letter confirming their status signed by their supervisors.




Please identify your name clearly and the Registration ID when ordering the transfer and send us a copy of the transfer receipt to events@i3s.up.pt.
The receipts will be issued only if requested and amendments will not be accepted.
Full payment of costs should be made in EURO (payments in any other currency will not be accepted).
Currency exchanges or bank fees have to be paid by the registrant. In case of cancellation, the registration fee will not be refunded.



Bank Holder Name: INEB - Instituto Nacional de Engenharia Biomédica
Bank: Millennium Bcp
Address Bank: Palácio do Comércio - Rua Sá da Bandeira, 501 - 4000-436 Porto Portugal
IBAN: PT50 0033 0000 0388 0160 11604
Account: 03880160116
INEB VAT number: 502 312 220


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4150-169 Porto
Tel.: +351 226 075 059 | Fax.: +351 226 063 339
E-mail: booking.ipanemaporto@hfhotels.com
Webpage: https://ibiointerface2019.hfhotels.com/corporate/

Participants have access to 10% discount on accommodation. 


Rua Arquitecto Marques da Silva, 200
4150-483 Porto - Portugal
Tel: +351 226 194 100 Fax: +351 226 194 160
E-mail: porto@hfhotels.com
Webpage: https://ibiointerface2019.hfhotels.com/corporate/

Participants have access to 10% discount on accommodation. 


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4150 Porto - Portugal
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Rua do Rosário, 160
4050-521 Porto - Portugal
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