Where Ideas Grow

Biointerfaces and Nanotechnology

The Biointerfaces and Nanotechnology (BN) core facility is part of the i3S and INEB (an institution with experience and international recognition in the biomaterials and biomedical fields), and it is open to all academic researchers and industry on a fee-for-service basis. Our aim is the study of materials, materials surfaces and interfaces of materials with cells and tissues, going down to the micrometric and nanometric level through the development and the improvement of advanced physical, chemical, mechanical and structural characterization techniques. We are particularly focused on the fields of Biomaterials and Nano- and Regenerative Medicine. BN technical staff also provides advanced training and education in specialized areas.

 

Resources available include:

  • non-destructive chemical characterization (Fourier Transformed Infra-red Spectroscopy);
  • mechanical characterization (Dynamic Mechanical Analyzer and Rheometer);
  • nanoparticle characterization in solution (through the determination of particle size, particle charge, isoelectric point and melting point);
  • macro characterization of solids surface electric charge;
  • determination of the thickness of ultrathin films on reflective surfaces (Ellipsometry with Image);
  • quantification of surface wettability and surface free energy of solid surfaces (Contact Angle Measuring Device);
  • Analysis of surface topography, force spectroscopy and nanoindentation of synthetic and biological samples (Atomic Force Microscope couple with an Inverted Fluorescence Microscope).

Users are invited to follow up their analysis carried out at BN, which also provides advanced training, in the specific cases. A Quality System is implemented at BN, according to ISO standards and Good Laboratory Practices (GLP). This contributes to ensure the provision of strict procedures followed at BN core facility, thus enabling each technique to be performed according to high quality standards. BN was created in 2005 and the platform's daily activities are supported by our technical staff and its governance is assured by a Scientific Coordinator. Since one of our objectives is the continuous improvement, we look forward to receiving your input (info.bn@i3s.up.pt).

Team: Manuela Brás - Head | Dalila Pedro - Team Member | Ricardo Vidal - Team Member | Cristina Martins - Scientific Coordinator

Contacts: mbras@i3s.up.pt | dalila.pedro@ineb.up.pt | rvsilva@ineb.up.pt (Lab 004S2/ Office: 004S4) (Ext. 6057/ 6058)

Platform Head

Team

Home

The Biointerfaces and Nanotechnology (BN) core facility is part of the i3S and INEB (an institution with experience and international recognition in the biomaterials and biomedical fields), and it is open to all academic researchers and industry on a fee-for-service basis. Our aim is the study of materials, materials surfaces and interfaces of materials with molecules, cells and tissues, going down to the micrometric and nanometric level. These studies are possible in our platform with the development and the improvement of advanced structural, chemical, physical and mechanical characterization techniques. The platform is particularly focused on the fields of Bioengineering, specifically in Biomaterials and Nano- and Regenerative Medicine. BN technical staff also provide advanced training in specialized areas.

Resources available include:

  • chemical characterization (Fourier Transformed Infra-red Spectroscopy);
  • mechanical characterization (Dynamic Mechanical Analysis and Rheology);
  • nanoparticle characterization in solution, through the determination of particle size, particle charge, isoelectric point and melting point (Dynamic Light Scattering and Laser Doppler Electrophoresis);
  • characterization of macro solids surface electric charge (Electro Kinetic Analysis);
  • determination of the thickness of ultrathin films on reflective surfaces until down to 10 nm (Imaging Ellipsometry);
  • quantification of surface wettability and surface free energy of solid surfaces (Contact Angle Measurements);
  • Analysis of surface topography, force spectroscopy and nanoindentation of biological samples (Atomic Force Microscope couple with an Inverted Fluorescence Microscope).

Users are invited to follow up their analysis carried out at BN, which also provides advanced training, in specific cases. If the analysis, required by the users, is necessary in a regular basis, the users are encouraged to learn the technique, in order to be independent users and use the platform as “Qualified users”. A Quality System is implemented at BN, according to ISO standards and Good Laboratory Practices (GLP). This contributes to ensure the provision of strict procedures followed at BN core facility, thus enabling each technique to be performed according to high quality standards. BN was created in 2005 and the platform's daily activities are supported by our technical staff and its governance is assured by a Scientific Coordinator. Since one of our objectives is the continuous improvement, we look forward to receive your feedback and suggestions (info.bn@i3s.up.pt).

Team: Manuela Brás - Head | Ricardo Vidal - Team Member | Cristina Martins - Scientific Coordinator

Contacts: mbras@i3s.up.pt | rvsilva@ineb.up.pt (Lab 004S2/ Office: 004S4) (Ext. 6057/ 6058)

User Policy

1 - Introduction

The Biointerfaces and Nanotechnology (BN) scientific platform provides access to advanced equipment for structural, chemical, physical and mechanical characterization of materials, surfaces and interfaces of materials with molecules, cells and tissues, at nano/micrometric scale.  This platform mainly supports the research in bioengineering and nanotechnology, namely in biomaterials applied to tissue repair and regeneration.

 

2 - Aim

 

BN scientific platform offers:

  • advanced training to i3S users in the equipment/techniques exiting at BN platform to create independent qualified users;
  • technical services to support research projects from researchers of i3S, other research centers, universities and industry;
  • organization of advanced courses in equipment and techniques with interest to the BN platform users.

 

3 - BN working conditions

 

The BN platform is supported by specialized technicians for the different available equipment/techniques. There are four established categories of clients, in which, the usage of the equipment has always an associated fee, which varies according to the type of entity requesting the service.

  1. i3S users;
  2. External academic University of Porto (UP) users;
  3. External academic non-UP users;
  4. External non-academic service users.

The specific fees are available at the BN's Webpage.

 

3.1 – i3S users

An internal client is considered any researcher that belongs to the i3S. There are two types of internal clients:

 

  • Qualified users – the i3S researchers that have qualifications to run the equipment independently, having received specialized training on the equipment by the technician in charge
  • Non-qualified users – the researchers that do not have qualifications to run the equipment independently, being necessary the support of the responsible specialized technician to perform the assay.

 

Note: A researcher that is not from i3S can be trained to be a qualified user if there a valid justification for that.

All i3S users are encouraged to receive training and therefore became qualified users; only when the assay will be either very short or very sporadic, the i3S user does not need to become a qualified user.

 

The qualified users must:

  • reserve the equipment in the scheduler of the i3S portal (portal.i3s.up.pt) (the use of equipment without prior reservation is not allowed);
  • cancel the reservation (as soon as possible) if are unable to attend (sessions not canceled before the time of booking will be paid);
  • follow the standard operating procedures established for each equipment;
  • perform the assays in accordance to the quality management requirements implemented at BN platform;
  • leave the equipment in optimal conditions for the following users;
  • record each session in the registration book available for each equipment;
  • keep the equipment and the working areas clean;
  • copying their own data (it is not allowed the use of USB storage devices connected to the computers, so the data must be saved in each user profile or project public disk except for Electrokinetic Analyzer (EKA) and Optical Contact Angle measurement device (OCA)).
  • switch off the equipment if nobody is going to use it afterwards
  • check the equipment shutdown (including computers and monitors) before leaving the BN platform if she/he is the last user of the day
  • INFORM THE TECHNICIAN-IN-CHARGE IMMEDIATELY about any problem found with the equipment
  • RECORD any problem found with the equipment in the equipment log book.

 

3.2 – External customers

To request a service, external customers should contact the person responsible of the BN scientific platform by phone and/or email (info.bn@i3s.up.pt.

The technician responsible for the characterization technique will send the pre-requisites and the quotation related to the assay. If the client accepts the analysis conditions, a contract information document will be prepared between the BN scientific platform and the client.  The contract document will be confirmed by signature or by email. The report with the discussion of the results is not included in the listed prices, but can be available on a surplus fee.

 

4- Quality Management

A Quality Management System is implemented at BN platform according to Good Laboratory Practices (GLP). This contributes to ensure the provision of strict procedures followed at BN scientific platform, thus enabling each technique to be performed according to high-quality standards.

 

5 – Location

 

BN scientific platform is located at the i3S – Instituto de Investigação e Inovação em Saúde, building at R. Alfredo Allen, 208, 4200-135 Porto.

 

 

6 – How to access the BN scientific platform and equipment

 

To know more about the available techniques and equipment, please contact the BN scientific platform, by phone (+ 351 22040 88 00/ 15), by e-mail (info.bn@i3s.up.pt), or through the websitehttps://www.i3s.up.pt/scientific-platform.php?v=57

Applications

Atomic Force Microscope coupled with Inverted Fluorescence Microscope

Life and Health sciences:

  • 2D and 3D topographic imaging of molecules, cells, tissues and microorganisms;
  • Force Spectroscopy and Molecular recognition with the quantification of binding forces in ligand-receptor 
  • Cell-cell adhesion determination binding forces
  • Mechanical properties quantification in biological samples
  • Roughness and morphometric parameters quantification

Polymer Industry

  • Imaging and roughness measurements of surface

Biosensors and electronic industry

  • Imaging and roughness measurement of surfaces

Optical Contact Angle Measurement Device

  • Life and Health Sciences
  • Polymer Industry
  • Surface characterization (according to the chemical groups existing in the surface)
  • Agrochemical industry (the effectiveness of a sprayed liquid will be determined by the degree to which it coats and adheres to the surface
  • Shoes and clothes industry (wettability of leather and tissues surface)
  • Materials

Fourier Transformed Infra Red Spectrometer

  • Biology (adsorption of proteins, and other layers in a surface)
  • Materials (adorption of organic layers in ceramics, metals, ...)
  • Geology 
  • Film analysis
  • Identification of contaminants
  • Food industry (identification of trans fat content of manufactured food products)
  • Forensic sciences (identification of chemical information in residues of fibers, coatings, paints, ….)
  • Pharmaceutical field (formulation development, validation and quality control)

Ellipsometer with image

These equipment allows studies in different areas:

  • Biology (thickness of film proteins and natural polymers)
  • Biomaterials (quantification of thickness of thin oxide films formed on metallic biomaterials by surface treatment)
  • Refractive substrates (Films in a nanometric scale, namely self-assembled monolayers - SAMs,  deposited on a reflective substrate)
  • Semiconductors physics to microelectronics
  • Quality control (films metrology)
  • Flat panel display industry
  • Optical lenses
  • Photovoltaics thin films

 

Zetasizer Nano ZS

  • Study of nanoparticles (biomaterials, liposomes, …) formulation for health therapies: size and charge
  • Protein conformation data: size
  • Environmental strategies to address pollution: size
  • Formulation stability; Proteins and polymer characterization: size, charge and isoeletric point
  • Quantum dots analysis: size

Zeta Potential Electro Kinetic Analyzer 

  • Quantification of Zeta potential at different pH (applied to solid materials: powders, membranes, granules, plates, …)
  • Materials Science (bulk solid, film, fiber,…), DMA applies different deformation modes: tension, compression, shear, single and/or dual cantilever bending and 3D point bending. The deformation modes can be applied resulting from changes in five experimental variables: temperature, time, frequency, force, and strain.

Rheometer

It allows the application of 4 different modes of assays: viscometry, oscillation, creep and recovery and stress and relaxation in different areas:

  • Life sciences and Health (mechanics of biological tissues)
  • Materials Science (hydrogels,…)
  • Coatings and food Industry (paints and chocolate, respectively
  • Pharmaceutical and cosmetic industry (liquids, ointments, creams, pastes, ….)

Dynamic Mechanical Analyser

  • Materials Science (bulk solid, film, fiber,…), DMA applies different deformation modes: tension, compression, shear, single and/or dual cantilever bending and 3D point bending. The deformation modes can be applied resulting from changes in five experimental variables: temperature, time, frequency, force, and strain.

Resources

Atomic Force Microscope coupled with Inverted Fluorescence Microscope

The Atomic Force Microscope (AFM) is a surface characterization technique using a cantilever, based in the surface. This technique provides: i) 3D topographic imaging, surface roughness and morphometric studies, at the nanometric scale, ii) single molecule binding forces determination using Force Spectroscopy and molecular recognitions and iii) determination of mechanical properties (ex: stiffness, Apparent Young’s modulus, adhesion, deformation (indentation)) in physiological conditions. Our AFM (Pico Plus 5500, keysight Technologies, USA) performs the following modes: Contact, Non-Contact, and Intermittent-contact (Tapping® mode and Magnetic Acoustic Mode – MAC™). The MAC Mode™ is generally recognized as the AFM “gentle” mode and consists in a magnetic cantilever directly controlled by a magnetic field. It integrates the oscillating mode (Acoustic AC) and the phase imaging (digital) as well. This mode is indicated for measurements in fluids, being characterized by low contact forces applied in extremely delicate samples, such as: proteins, DNA, RNA, cells and other biological structures. The AFM is coupled to an Inverted Fluorescence Microscope (IFM) (Observer Z1, Zeiss, Germany). The system allows a variability of assays in biological samples. It has four objectives: 10X air, 40X oil, 63X oil and 100 X oil. It has filters to work with DAPI and eGFP fluorophores. It has Differential Interference Contrast (DIC). It does not permit to work with Phase contrast.

 

Responsible for the assays: Manuela Brás

 

Optical Contact Angle

The principle of this equipment is based in the optical contact angle (OCA) (OCA, Data Physics, USA) measurement between a liquid drop and a surface, being possible to use different measuring methods: pendant, sessile and inverted drop. Basically the drop is released from an automatic vertical needle through the sample, inside a chamber with temperature control. The contact angle is the angle, conventionally measured through the liquid, where a liquid-vapor interface meets a solid surface. It quantifies the wettability of a solid surface by a liquid via the Young’s equation. A given system of solid, liquid, and vapor at a given temperature and pressure has a unique equilibrium contact angle. This apparatus also allows the determination of the surface tension of pure liquids as well as with protein containing solutions. The equipment quantifies the free energy of a surface material, which is a very important parameter in material’s characterization. This technique also allows the determination of specific biomolecule’s affinity, such as proteins on a surface, by the calculation of the adhesion work.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vida

 

Dynamical Mechanical Analyzer

The DMA is used to study and characterize materials, being most useful for observing the mechanical properties and viscoelastic nature of polymers. The DMA can determine changes in sample properties applying different deformation modes: tension, compression, shear, single, dual cantilever bending and 3D point bending. The deformation modes can be applied resulting from changes in five experimental variables: temperature, time, frequency, force, and strain. The DMA uses samples that can be in bulk solid, film, fiber, gel, or very viscous liquid form.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Ellipsometer

Ellipsometry is an optical technique for investigating the dieletric properties (complex refractive index or dieletric function) of thin films. Ellipsometry measures the change of polarization of light upon reflection or transmission and compares it with a model. Our Ellipsometer (EP3, Accurion, Germany) is able to determine the refraction index and extinction coefficient index, and with these, the thickness of thin films covering reflecting surfaces. The equipment uses a 532 nm laser and allows the possibility of adjusting the angle of incidence for measurements, to carry out the following studies: i) Films in a nanometric scale, namely self-assembled monolayers - SAMs, polymeric films and ceramics, deposited on a reflective substrate; ii) Analysis of in situ and in real time material adsorption on the previously indicated films or reflective substrates (using a liquid cell); iii) Quantification of thickness of thin oxide films formed on metallic biomaterials by surface treatment.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Fourier Transformed Infra-Red Specctrometer

It is a vibrational spectroscopy technique that provides information about molecular structure, through the identification of functional groups and chemical bonds of molecules. It also allows quantification methods. The equipment may analyse solid samples (powders, dense materials, thick films, gels) and liquids. In case of FTIR spectroscopy, the available accessories allow for different analytical methods, namely:

  • Transmittance
  • ATR (Attenuated Total Reflectance)
  • IRRAS (Infra- Red Reflectance Absorption Spectroscopy) for films with a monolayer thickness, in reflective surfaces


Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal/ Manuela Brás

 

Zetasizer Nano ZS

Zetasizer Nano ZS has the ability to measure characteristics of particles or molecules dispersed in a liquid medium, namely particle size and zeta potential, in a wide range of concentrations. The Zetasizer Nano ZS is equipped with a MPT-2 autotitrator, a 633 nm laser and allows the temperature control from 10 to 90 ºC.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Rheometer Kinexus

The Kinexus Rheometer is used to study and characterize rheological properties of materials, being most useful for observing the viscosity and viscoelasticity of polymers. The Rheometer allows the application of 4 different modes of assays: viscometry, oscillation, creep and recovery and stress and relaxation. It determines then, changes in sample properties resulting from changes in external conditions applied, such as stress, strain, timescale and temperature. The rheometer uses samples that can be in bulk solid, molten, semi-solid (such as pastes, creams and gels) or viscous to dilute liquid solutions.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Zeta Potential Electro Kinetic Analyzer

The Electro Kinetic Analyzer (EKA) can be used to investigate the zeta potential of macroscopic solids based on a streaming potential and streaming current measurement. The zeta potential is related to the surface charge at a solid/liquid interface and it is a powerful indicator for the surface chemistry and liquid phase adsorption processes. It is therefore an interfacial property of great importance to understand the behaviour of solid materials in several biological/technical processes. The EKA instrument has five different measuring cells for flexible sample mounting, which may accommodate macroscopic solids of almost any size and shape, namely planar solid samples, films, fibers, foils, granular particles and powders.
Quotation: under request

 

 

Responsible for the assays: Ricardo Vidal

 

Synergy Mx

Fully monochromator-based microplate reader for high throughput multi-detection readings. High performance fluorescence, absorbance and luminescence detection methods. 

  • Kinetics readings
  • Area scans
  • Spectral analysis
  • Temperature control
  • Shaker

 

 

Responsible for the assays: Dalila Pedro

Services

Services

There are two categories of clients: internal and external. The clients can use the facilities on a fee –for-service basis (under revision, please contact the technical staff).

A Quality System is implemented at BN, according to ISO standards and Good Laboratory Practices (GLP). This contributes to ensure the provision of strict procedures followed at BN facility, thus enabling each technique to be performed according to high quality standards. Prices are under revision.

Rules for Users

Advanced training is given by expert technicians in each characterization technique. Regarding the training the users are classified in two categories:

  • Qualified users – the researchers that have qualifications to run the equipment independently, having received specialized training on the equipment by the technician in charge
  • Non-qualified users – the researchers that do not have qualifications to run the equipment independently, being necessary the support of the responsible specialized technician to perform the assay.

Note: If a non-qualified user requests a long duration assay, the specialized technician responsible for the equipment will provide the specific training and the trainee will continue the assays by her/ himself as a qualified user.

All clients are encouraged to receive training and therefore became qualified users; only when the assay will be either very short or very sporadic, the client can be non-qualified user.

The qualified users have the following duties:

  • they must do the equipment reservation in the scheduler of the i3s portal (portal.i3s.up.pt), after the training period being considered enough and approved by the responsible technician;
  • It is forbidden to use the facility without booking;
  • ALL qualified users are required to work under our standard operating procedures;
  • the pre-requisites for the assays are known and understood;
    the tests must be done according to the Quality management requirements implemented at BN;
  • qualified users must register and sign the registry equipment file;
  • qualified users are responsible for leaving the equipment in optimal conditions for the following users;
  • the preservation of samples is responsibility of the qualified users;
  • the results obtained are not subjected to a report, unless it is requested in the beginning of the assay, to the technician in charge of the equipment;
  • qualified users have to record each session in the registration book available for each equipment and keep the equipment and the working areas clean;
  • qualified users must switch off the equipment if nobody is going to use it afterwards;
  • if a qualified user books a session and then decides not to attend, the user must cancel the reservation as soon as possible; this will assure the optimization of the equipment usage;>
  • qualified users are responsible for copying their own data (VERY IMPORTANT: check the storage device for viruses before CONNECTING TO THE COMPUTERS OF THE FACILITY). It is recommended, if applicable, to send data by e-mail or save in profile disk, if possible Qualified users should always accompany the shutdown procedure of the equipment until the end and not leave the computers/ monitors on, when being the last user of the day;
  • if qualified users do not use the time scheduled in the equipment and did not cancel the reservation on time, the session will be paid nevertheless.

WARNING: PLEASE INFORM THE TECHNICIAN-IN-CHARGE IMMEDIATELY about any problems you might have had with the equipment or the workstations, for them to be fixed as soon as possible!! You can do it personally or by the email

Output and Acknowledgements

Please send us the reference of your published work in which you have used our scientific platform. That information will appear in our Publications section. For acknowledgement purposes see the guidelines at: https://portal.i3s.up.pt/index.php?id=142. This is mandatory in all the scientific production where this scientific platform was used.

Training

Past Training Actions

Course Atomic Force Microscopy applied to Life Sciences | 2nd Ed.

6-8 November 2019 | i3S

Atomic Force Microscopy (AFM) has revealed as a powerful tool to study human pathology, in fields ranging from cancer, cardiovascular and infection diseases, since it is suitable to perform studies on different molecules, cell/ tissue types at physiological conditions. The main principle is based in the interatomic forces established between a probe tip and the sample causing the cantilever to deflect as the samples’ surface topography changes. Laser light reflected from the back of the cantilever measures the deflection of the cantilever and the force applied.

In this lab session, the participants will be introduced to the AFM/IFM techniques, having the opportunity to learn about the determination of morphometric parameters to the characterization of biomechanical properties of cells.

As well, students will learn how to use different software for the analysis of the data arising from these studies. Applets software will be used to analyze the Thermal tune data to obtain the force constant of the cantilevers and to analyze AFM force-distance curves, to calculate the mechanical properties of the samples.

 

Workshop in Atomic Force Microscopy coupled to Inverted Fluorescence Microscopy
15-17 November 2017 | i3S, Lab 222 S2 and Room 2

Atomic Force Microscopy (AFM) has revealed as a powerful tool to study human pathology, in fields ranging from cancer, cardiovascular and blood diseases, since it is suitable to perform studies on different molecules, cell/ tissue types at physiological conditions. The main principle is based in the interatomic forces stablished between a probe tip and the sample causing the cantilever to deflect as the samples’ surface topography changes. A laser light reflected from the back of the cantilever measures the deflection of the cantilever. In this lab session the participants will be introduced to the AFM/IFM techniques, having the opportunity to learn about the determination of morphometric parameters to the characterization of biomechanical properties of cells. As well, students will learn how to use different software for the analysis of the data arising from these studies. Applets software will be used to analyze the Thermal tune data to obtain the force constant of the cantilevers and to analyze AFM force distance curves, to calculate the mechanical properties of the samples.

 

Publications

Publications

Selvaggio G, Canato S, Pawar A, Monteiro PT, Guerreiro PS, Brás MMJanody F, Chaouiya C., Hybrid Epithelial-Mesenchymal Phenotypes Are Controlled by Microenvironmental Factors. Cancer research 80: 2407-2420, 2020; doi: 10.1158/0008-5472.CAN-19-3147 PMID: 32217696

Sara I. Faria, Rita Teixeira-Santos, Luciana C. Gomes, Elisabete R. Silva, João Morais, Vítor Vasconcelos  and Filipe J. M. Mergulhão. Experimental Assessment of the Performance of Two Marine Coatings to Curb Biofilm Formation of Microfoulers. Coatings 2020, 10, 893; doi:10.3390/coatings10090893.

Bruna Costa, Rita Mota, Paula Tamagnini, M. Cristina L. Martins and Fabíola Costa. Natural Cyanobacterial Polymer-Based Coating as a Preventive Strategy to Avoid Catheter-Associated Urinary Tract Infections, Mar. Drugs 2020, 18, 279; doi:10.3390/md18060279

Patrícia C. Henriques, Andreia T. Pereira, Ana L. Pires, André M. Pereira, Fernão D. Magalhães and  Inês C. GonçalvesGraphene Surfaces Interaction with Proteins, Bacteria, Mammalian Cells, and Blood Constituents: The Impact of Graphene Platelet Oxidation and Thickness, ACS Appl. Mater. Interfaces 2020, 12, 18

Parreira P, Monteiro C, Graça V, Gomes J, Maia S, Gomes P, Gonçalves IC, Martins MCL.  Immobilized Antimicrobial Peptides for Gastric Infection Management. Scientific Reports. 2019. 9:18212

Monteiro C, Costa F, Pirttilä AM, Tejesvi MV, Martins MCL, Prevention of urinary catheter aciated infections by coating antimicrobial peptides from crowberry endophytes. Scientific Reports. 2019.9. 10753

Andreia T. Pereira, Patrícia C. Henriques, Paulo C. Costa, Maria Cristina L. Martins, Fernão D. Magalhães, Inês C. Gonçalves. Graphene oxide-reinforced poly(2-hydroxyethyl methacrylate) hydrogels with extreme stiffness and high-strength. Composites Science and Technology 2019;184:107819.

Costa B, Mota R, Parreira P, Tamagnini P, Martins MCL, Costa F. Broad-Spectrum Anti-adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium. Marine Drugs. 2019 Apr 24;17(4). pii: E243. doi: 10.3390/md17040243

Costa B, Mota R, Parreira P, Tamagnini P, Martins MCL, Costa F. Broad-Spectrum Anti-adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium. Marine Drugs. 2019 Apr 24;17(4). pii: E243. doi: 10.3390/md17040243sso

Barros D, Conde-Soua E, Gonçalves AM, Han WM, García AJ, Amaral IF, Pêgo AP. Engineering Hydrogels with Affinity-Bound Laminin as 3D Neural Stem Cell Culture Systems. Biomaterials Science 2019; 7, 5338-5349 (DOI: 10.1039/C9BM00348G) 

Barros D, Parreira P, Furtado J, Ferreira-da-Silva F, Conde-Sousa E, Garcia AJ, Martins MCL, Amaral IF, Pêgo AP. An affinity-based approach to engineer laminin-presenting cell instructive microenvironments. Biomaterials. 2019; 192:601-11. (DOI:10.1016/j.biomaterials.2018.10.039).

Catarina C. Coelhoa, Rita Araújo, Paulo A. Quadros , Susana R. Sousa, Fernando J. Monteiroa. Antibacterial bone substitute of hydroxyapatite and magnesium oxide to prevent dental and orthopaedic infections, Materials Science and Engineering: CVolume 97, April 2019, Pages 529-538

D. Barros, P. Parreira, J. Furtado, F. Ferreira-da-Silva, E. Conde-Sousa, A.J. García, M.C.L. Martins, I.F. Amaral, A.P. Pêgo, An Affinity-Based Approach to Engineer Laminin-Presenting Cell Instructive Microenvironments, Biomaterials, 192. 2019, 601–611. https://doi.org/10.1016/j.biomaterials.2018.10.039

Elisa Santovitoa, Jose das Neves , Donato Grecoa, Vito D’Ascanioa, Bruno Sarmentob, Antonio Francesco Logriecoa and Giuseppina Avantaggiato. Antimicrobial properties of rosin acids-loaded nanoparticles against antibiotic-sensitive and antibiotic-resistant foodborne pathogens. ARTIFICIAL CELLS, NANOMEDICINE, AND BIOTECHNOLOGY 2018, VOL. 46, NO. S3, S414–S422 https://doi.org/10.1080/21691401.2018.1496924

Rita N. Gomes, Ines Borges, Andreia T. Pereira, Andre F. Maia, Manuel Pestana, Fernao D. Magalhães , Artur M. Pinto , Ines C. GonçalvesAntimicrobial graphene nanoplatelets coatings for silicone catheters. Carbon Volume 139, November 2018, Pages 635-647

Gomes, C. P., Leiro, V., Lopes, C. D., Spencer, A. P., Pêgo, A. P. Fine tuning neuronal targeting of nanoparticles by adjusting the ligand grafting density and combining PEG spacers of different length. Acta Biomaterialia. (2018).

P.M.D. Moreno, A.R. Ferreira, D. Salvador, M.T. Rodrigues, M. Torrado, E.D. Carvalho, U. Tedebark, M.M. Sousa, I.F. Amaral, J. Wengel, A.P. Pêgo, Hydrogel-assisted antisense LNA gapmer delivery for in situ gene silencing in spinal cord injury, Molecular Therapy: Nucleic Acid, 2018. DOI: 10.1016/j.omtn.2018.03.009

Carla P. Gomes, Cátia D. F. Lopes, Michael Leitner, Andreas Ebner, Peter Hinterdorfer, and Ana P. Pêgo. Atomic Force Microscopy as a Tool to Assess the Specificity of Targeted Nanoparticles in Biological Models of High Complexity, Adv. Healthcare Mater. 2017, 1700597.

Karabiyik C, Fernandes R, Figueiredo FR, Socodato R, Lambertsen KL, Relvas JB, Santos SD. 2017 Neuronal Rho GTPase Rac1 elimination confers neuroprotection in a mouse model of permanent ischemic stroke. Brain Pathol.

Leiro, V., Garcia, J.P., Moreno, P., Spencer, A.P., Fernandez-Villamarin, M., Riguera, R., Fernandez-Megia, E., Pêgo, A.P. 2017 “Biodegradable PEG-GATGE dendritic block copolymers: Synthesis and Biofunctionality Assessment as Vectors of siRNA”. Journal of Materials Chemistry B. 5, 4901-4917.

Costa F, Sousa DM, Lamghari M, Gomes P, Martins MCL. N-acetylcysteine-functionalization coating avoids bacterial adhesion and biofilm formation. Scientific Reports. 2017; 7;17374; 1-13

Felgueiras HP, Wang LM; Ren KF; Querido M; Jin Q; Barbosa MA; Ji J; Martins MCL. Octadecyl chains immobilized onto hyaluronic acid coatings by thiol-ene "click chemistry" increase the surface antimicrobial properties and prevent platelet adhesion and activation to polyurethane. ACS Applied Materials and Interfaces. 2017; 9 (9) 7979–7989

Seabra CL, Nunes C, Gomez-Lazaro, Correia M, Machado JC, Gonçalves IC, ReisCA,Reis S, Martins MCL.Docosahexaenoic acid loaded lipid nanoparticles with bactericidal activity against Helicobacter pylori. International Journal of Pharmaceutics. 2017; 519; 128-137

J.R. Dias, S. Baptista-Silva, C.M.T. de Oliveira, A. Sousa, A.L. Oliveira, P.J. Bártolo, P.L. Granja, In situ crosslinked electrospun gelatin nanofibers for skin regeneration, European Polymer Journal, Volume 95, 2017, Pages 161-173

Castro F, Pinto ML, Silva AM, Pereira CL, Teixeira GQ, Gomez-Lazaro M, Santos SG, Barbosa MA, Gonçalves RM, Oliveira MJ. Pro-inflammatory chitosan/poly(γ-glutamic acid) nanoparticles modulate human antigen-presenting cells phenotype and revert their pro-invasive capacity. Acta Biomater. 2017 Nov;63:96-109.

Antunes JC, Pereira CL, Teixeira GQ, Silva RV, Caldeira J, Grad S, Gonçalves RM, Barbosa MA, Poly(γ-glutamic acid) and poly(γ-glutamic acid)-based nanocomplexes enhance type II collagen production in intervertebral disc.J Mater Sci Mater Med. 2017 Jan;28(1):6

Andreia M. Silva, Maria I. Almeida, José H. Teixeira, André F. Maia, George A. Calin, Mário A. Barbosa, Susana G. Santos 2017, Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment. Under minor revision in Scientific Reports.

Antunes JC, Pereira CL, Teixeira GQ, Silva RV, Caldeira J, Grad S, Gonçalves RM, Barbosa MA, Poly(g-glutamic acid) and poly(g-glutamic acid)-based nanocomplexes enhance type II collagen production in intervertebral disc, J Mater Sci Mater Med. 2017;28(1):6.

Oliveira MI, Pinto ML, Gonçalves RM, Martins MC, Santos SG, Barbosa MA. Adsorbed Fibrinogen stimulates TLR-4 on monocytes and induces BMP-2 expression. Acta Biomater. 2017 Feb;49:296-305.

I.C. Gonçalves, A. Magalhães, A.M.S. Costa, J.R. Oliveira, P.C. Henriques, P. Gomes, C.A. Reis, M.C.L. Martins. Bacteria-targeted biomaterials: glycan-coated microspheres to bind Helicobacter pylori. Acta Biomaterialia 2016; 33:40–50

Teixeira GQ, Leite Pereira C, Castro F, Ferreira JR, Gomez-Lazaro M, Aguiar P, Barbosa MA, Neidlinger-Wilke C, Goncalves RM, Anti-inflammatory Chitosan/Poly-?-glutamic acid nanoparticles control inflammation while remodeling extracellular matrix in degenerated intervertebral disc. Acta Biomater. 2016; 42:168-79

Daniel M. Vasconcelos, Raquel M Gonc?alves, Catarina R Almeida, Ine?s O Pereira, Marta I Oliveira, Nuno Neves, Andreia M Silva, Anto?nio C Ribeiro, Carla Cunha, Ana R Almeida, Cristina C. Ribeiro, Ana M. Gil, Elisabeth Seebach, Katharyna L. Kynast, Wiltrud Richter, Meriem Lamghari, Susana G Santos and Ma?rio A Barbosa, "Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration”, Biomaterials 2016;111:163-178.

Cunha-Reis C, Machado A, Barreiros L, Araújo F, Nunes R, Seabra V, Ferreira D, Segundo MA, Sarmento B, das Neves J. Nanoparticles-in-film for the combined vaginal delivery of anti-HIV microbicide drugs. J Control Release. 2016 Dec 10;243:43-53.

Braz L Grenha A, Ferreira D, Rosa da Costa AM, Gamazo C, Sarmento B, Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization. Int J Biol Macromol. 2016 Dec 31;96:786-797.

Abreu CM, Paula HC, Seabra V, Feitosa JP, Sarmento B, de Paula RCSynthesis and characterization of non-toxic and thermo-sensitive poly(N-isopropylacrylamide)-grafted cashew gum nanoparticles as a potential epirubicin delivery matrix. Carbohydr Polym. 2016 Dec 10; 154: 77-85.

Goes CP, Varela-Moreira A, Leiro V, Lopes CD, Moreno PM, Gomez-Lazaro M, Pêgo AP. A high-throughput bioimaging study to assess the impact of chitosan-based nanoparticle degradation on DNA delivery performance. Acta Biomater. 2016 Dec;46:129-140.

Rodrigues F, Alves AC, Nunes C, Sarmento B, Amaral MH, Reis S, Oliveira MB. Permeation of topically applied caffeine from a food by-product in cosmetic formulations: Is nanoscale in vitro approach an option? Int J Pharm. 2016 Nov 20;513(1-2):496-503.

Fonte P, Andrade F, Azevedo C, Pinto J, Seabra V, van de Weert M, Reis S, Sarmento B. Effect of the Freezing Step in the Stability and Bioactivity of Protein-Loaded PLGA Nanoparticles Upon Lyophilization. Pharm Res. 2016 Nov;33(11):2777-93.

Sgorla D, Bunhak ÉJ, Cavalcanti OA, Fonte P, Sarmento B. Exploitation of lipid-polymeric matrices at nanoscale for drug delivery applications. Expert Opin Drug Deliv. 2016 Sep;13(9):1301-9

Laranjeira MS, Moço A, Ferreira J, Coimbra S, Costa E, Santos-Silva A, Ferreira PJ, Monteiro FJ Different hydroxyapatite magnetic nanoparticles for medical imaging: Its effects on hemostatic, hemolytic activity and cellular cytotoxicity. Colloids Surf B Biointerfaces. 2016

Machado A, Cunha-Reis C, Araújo F, Nunes R, Seabra V, Ferreira D, das Neves J, Sarmento B Development and in vivo safety assessment of tenofovir-loaded nanoparticles-in-film as a novel vaginal microbicide delivery system. Acta Biomater. 2016 Oct 15;44:332-40.

Felgueiras HP,Murthy NS, , Brás MM, Migonney V, Kohn J, Competitive Adsorption of Plasma Proteins Using Quartz Crystal Microbalance. ACS Appl Mater Interfaces, 2016,

Ribeiro M, Ferraz MP, Monteiro FJ, Fernandes MH, Beppu MM, Mantione D, Sardon H. Antibacterial silk fibroin/nanohydroxyapatite hydrogels with silver and gold nanoparticles for bone regeneration. Nanomedicine. 2016 Aug 31; 13(1):231-239.

González-Delgado JA, Castro PM, Machado A, Araújo F, Rodrigues F, Korsak B, Ferreira M, Tomé JP, Sarmento B. Hydrogels containing porphyrin-loaded nanoparticles for topical photodynamic applications. Int J Pharm. 2016 Aug 20;510(1):221-31.

das Neves J, Nunes R, Rodrigues F, Sarmento B. Nanomedicine in the development of anti-HIV microbicides. Adv Drug Deliv Rev. 2016 Aug 1;103:57-75. doi: 10.1016/j.addr.2016.01.017

 

 

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