creating a
healthier future

12th Course on Optical Microscopy Imaging for Biosciences

6th-9th April 2021 | ONLINE COURSE


Imaging living cells are pivotal to understanding the biological processes. The course will present the state-of-art light microscopy techniques applied to study live cells and subcellular structures into different contexts (e.g., 2D and 3D culture systems, tissue explants, small model organisms, etc…). The program includes theoretical lectures headed by field specialists and live virtual practical modules which will offer the participants the opportunity to contact with cutting-edge microscopes (e.g., lattice light-sheet), learn how to manage live samples under the microscope and to get initiated into multidimensional image data analysis.

Target audience: The course is directed at master and PhD students, early career researchers and other researchers interested in acquiring skills in advanced light microscopy. 
Requirements: Basic knowledge of light microscopy and practice in fluorescence microscopy
Course duration: 20 hours
Course Organizers: Paula Sampaio, Maria Azevedo, Sven Terclavers






Introduction Microscopy - Michael Zoelffel

Sample preparation - Maria Azevedo
Experiment planing and selection of appropriated setup - Paula Sampaio


Confocal applications - Chris Power
Super resolution applications - Chris Power

Expansion Microscopy - Pedro Pereira
3D STED super-resolution - António Pereira
New insights on mitotic spindle assembly - a nanoscale perspective - Ana Almeida

3D image analysis tools - Thomas Boudier
Live virtual demonstration of LSM900 from Zeiss Customer Centre Oberkochen


Automation and HCS applications - Soren Prag
3D HCS in drug discovery applications - André Maia

Intravital microscopy reveals the plastic nature of cancer initiation and progression - Jacco van Rheenen 

Bioorthogonal labeling of transmembrane proteins: because size matters - Diogo Neto

Image Analysis automation - Mafalda Sousa
Machine learning - Sebastian Rhode

Practical: Live virtual demonstration of Celldiscoverer 7-LSM900 from Zeiss Customer Centre Oberkochen


Lightsheet imaging applications - Jacques Paysan
Lattice Lightsheet applications - Jacques Paysan
Lightsheet microscopy in clinical applications - Gertrude Bunt
TBA - Wesley Legant

Practical: Live virtual demonstration of Lattice Lightsheet 7 from Zeiss Customer Centre Oberkochen


Jacco van Rheenen, The Netherlands Cancer Institute, Amsterdam, The Netherlands
Wesley R. Legant, UNC School of Medicine-Chapel Hill, USA
Gertrude Bunt, University Medicine Göttingen, Göttingen, Germany
Pedro Matos Pereira, ITQB-NOVA, Oeiras, Portugal
Diogo Bessa-Neto, Interdisciplinary Institute for Neuroscience, Bordeaux, France
Michael Zoelffel, ZEISS Research Microscopy Solutions
Chris Power, ZEISS Research Microscopy Solutions
Soren Prag, ZEISS Research Microscopy Solutions
Jacques Paysan, ZEISS Research Microscopy Solutions
Mafalda Sousa, i3S, Porto, Portugal
Maria Azevedo, i3S, Porto, Portugal
André Maia, i3S, Porto, Portugal
Paula Sampaio, i3S, Porto, Portugal
António Pereira, i3S, Porto, Portugal
Ana Almeida, i3S, Porto, Portugal


Registration fee includes workshop participation and certificate.

i3S members – 75€
External members – 100€

Pre-registration form >>


Registration deadline: 26th March 2021

Payment will be requested only after confirmation of acceptance.
The organizer reserves the right to cancel the course in case of an insufficient number of participants or other unforeseeable events that render the execution of the course.



More Information
Advanced Training Unit | E-mail: | Tel: +351 220 408 800