Where Ideas Grow

Training School

Biomolecular condensates in cell function, aging and disease

18 - 22 April 2022 | i3S – Instituto de Investigação e Inovação em Saúde

 

The main goal of this training school is to raise awareness and provide expertise on fundamental aspects of phase separation and formation of biomolecular condensates, specifically covering the importance of this process to cellular biology and its contribution to the aging process and age-related diseases. Participants will learn basic concepts related to membrane-less organelles formation by protein phase separation and its regulation and will explore the experimental methods to study these processes in vitro. Trainees will work in groups and receive theoretical and practical hands-on training on methodologies and guidelines for experimental validation of protein phase separation. The program will include a career development workshop covering publication ethics and integrity.

 

Target audience: Master, Ph.D. Students and Early-stage researchers
Course duration: 35h
Course language: English 
Certificate: A certificate will be issued at the end of the course to participants that meet 80% of the course duration.
Requirements: Laptop

Organizing Committee:
Rita Vilaça | Monica Marques | Sandra Ribeiro

 

Poster >> 
 

Venue

The course will be held at i3S – Instituto de Investigação e Inovação em Saúde.
Rua Alfredo Allen, 208; 4200-135 Porto, Portugal
Tel: +351 220 408 800 | Site: http://www.i3s.up.pt/ | E-mail: events@i3s.up.pt
GPS coordinates: 41º 10’ 30.008’’ N, 8º 36’ 12.488’’ W.
 

Contents

Participants will acquire the following skills:
- Knowledge of databases of protein and RNA phase separation
- Knowledge of predictions of protein liquid-liquid phase separation
- Knowledge on functional and pathogenic phase separation in cell function and disease
- Knowledge of tools and methods to evaluate protein phase separation
 

Program

Preliminary Program

Lisbon Time

 

April 18  
Afternoon

14:00– 14:30 Arrival and registration

14:30-15:00 Welcome session (Sandra Macedo-Ribeiro, i3S)

15:00-16:00 Molecular Markers of Aging (Elsa Logarinho, i3S)

16:00-16:30 Coffee break

16:30-17:30 Introduction to the experimental analysis of protein phase separation (Joris Van Lindt, VIB)

17:30-18:00 Group work assignments

 

April 19 
Morning

9:00 – 10:30 Correlative Optical Tweezers to study molecular processes in real-time: from single molecules to biological condensates (Emma Verver, Lumicks)

10:30-10:45 Coffee break

10:45-12:00 Title to be announced (Emil Stender, FIDAbio)

12:00-13:00 Career Development: Publication ethics and integrity (Susana Magalhães, i3S)

13:00-14:00 Lunch

Afternoon

14:00-18:30 Practical sessions (with coffee break)


April 20

Morning

9:00 – 10:00 Phase separations and transitions by viral proteins: from viral factories to interference with host cell functions (Sonia Longhi, AFMB) - online

10:00-11:00 New paradigms in virology (Maria João Amorim, IGC) - online

11:00 – 11:30 Coffee break

11:30-12:30 Targeting phase separation in diseases (Peter Tompa, VIB)

12:30-14:00 Lunch

Afternoon

14:00-18:30 Practical sessions (with coffee break)

 

April 21 
 

Morning

9:15-10:00 Molecular Determinants of Selectivity in Disordered Complexes May Shed Light on Specificity in Protein Condensates (Alexander Monzon, UniPD)

10:00-11:00 Non-coding RNAs and phase separation rule the world! (Gian Tartaglia,IIT)

11:00-11:30 Coffee break

11:30-12:30 Biophysical models of protein aggregation and phase separation (Pedro Martins, i3S)

12:30-14:00 Lunch

Afternoon

14:00-15:00 Practical session

15:00-16:00 Chromatin and its phases (Serena Sanulli, Stanford University) - online

16:30-18:30 Social event

20:00 Dinner

 

April 22 
Morning

9:30-10:30 Group work session

10:30-11:30 Phase separation in biology and disease: Challenges and future perspectives (Ludo Van Den Bosch, VIB)

11:30-12:00 Coffee break

12:00-12:45 Liquid-liquid phase separation and stress granule dynamics in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD): challenges and future perspectives (Ludo Van Den Bosch, VIB) – i3S Friday Noon Seminar 

13:00-14:00 Lunch

Afternoon

14:00-16:00 Presentation of group work

16:00-16:30 Coffee break

16:00-17:00 Closing Session

 

Speakers

Sandra Ribeiro – i3S Instituto de Investigação e Inovação em Saúde, PT
Pedro Martins – i3S Instituto de Investigação e Inovação em Saúde, PT
Elsa Logarinho - i3S Instituto de Investigação e Inovação em Saúde, PT
Frederico Silva - i3S Instituto de Investigação e Inovação em Saúde, PT
Susana Magalhaes - i3S Instituto de Investigação e Inovação em Saúde, PT
Pedro Martins - i3S Instituto de Investigação e Inovação em Saúde, PT
Rita Vilaça - i3S Instituto de Investigação e Inovação em Saúde, PT
Zsuzsa Sárkány - i3S Instituto de Investigação e Inovação em Saúde, PT
André Maia - i3S Instituto de Investigação e Inovação em Saúde
Mónica Marques - i3S Instituto de Investigação e Inovação em Saúde, PT
Maria João Amorim - IGC Instituto Gulbenkian de Ciência, PT 
Ludo Van Den Bosch - VIB Vlaams Instituut voor Biotechnologie, BE
Peter Tompa - VIB Vlaams Instituut voor Biotechnologie, BE
Joris Van Lindt -VIB Vlaams Instituut voor Biotechnologie, BE
Salvador Ventura – Autonomous University of Barcelona, ES
Susanna Navarro - Autonomous University of Barcelona, ES
Silvio Tosatto - UniPD Università degli Studi di Padova, IT 
Alexander Monzon - UniPD Università degli Studi di Padova, IT 
Sonia Longhi - CNRS Center for the National Scientific Research, FR
Gian Tartaglia - IIT Istituto Italiano Di Tecnologia, IT
Serena Sanulli - Stanford University School of Medicine, EUA
Emma Verver – Lumicks, NL
Emil Stender – FIDAbio, DK

 

 

Registration

Registration fee includes coffee breaks, lunches, dinners (including social dinner on April 21), and course materials. 
Limited number of participants.


Fees: 
Early Bird | Until 25 March 2022
(Communication of acceptance on 26 March)
PhasAGE members – 65 € 
External Participants – 120 €
Non-Academic participants – 240€


Late Registration | Until 30 March 2022 (Communication of acceptance on 5 April)
PhasAGE members –  90€ 
External Participants –  180 €
Non-Academic participants – 360€
 

Registration form >>

Please note that your pre-registration does not confirm that you have been selected for the course.
Payment will be requested only after confirmation of acceptance. Details for payment will be sent in the confirmation registration e-mail.
The organizer reserves the right to cancel the course in case of an insufficient number of participants or other unforeseeable events that render the execution of the course.
i3S reserves the right to close registrations before the pre-announced closing dates if applications exceed the places available for the course.

 

Sponsors

     


     


More Information:
Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 226 074 900