Advanced Course on Organoid Models, 2nd Edition
21-25 November 2022 | i3S, Porto
This unique Advanced Course will combine the establishment of adult stem cells derived organoids with learning how to set them up on organs-on-chip systems.
The fundamentals of organoid models practical work will be addressed, and participants will learn how to culture ASCs derived intestinal, gastric and lung organoids. Moreover, they will be instructed on how to plan and run experiments using organoids and organs-on-chip. The specific protocols used for imaging, molecular biology and histology using organoids will be covered.
Target audicence: Master, PhD students and Early Stage Researchers
Course approximate duration: 35 hours
Requirements:
Prior knowledge required:
- Basic training in Biology, Bioengineering or related sciences;
- Cell culture techniques knowledge;
- Molecular biology techniques knowledge.
Course organizers: Anna Olsson, i3S; Marta Alves da Silva, i3S
Program
Participants will acquire the following skills:
- establish and maintain cultures of intestinal, gastric and lung organoids;
- learn how to design and plan experiments using organoids;
- learn how to choose, set up and work with organs on chips;
- prepare organoids samples for histological analysis;
- analyze practical outcomes of their cultures by imaging techniques (brightfield and confocal microscopy).
Detailed Program
Lectures will take place in Corino de Andrade Auditorium
21st November, 2022
8:45 - 9:00 | Registration
9:00 - 9:30 | Welcome and flash presentations by participants
9:30 - 10:15 | Stem cells and Organoids Biology
Marta Alves da Silva
10:15 - 11:00 | TE Principles and Organoids
Cristina Barrias
11:00 - 11:30 | Coffee Break
11:30 - 12:15 | Organoids in Cancer Research
Celso Reis
12:15 - 13:00 | Organoids in Infection
Margarida Saraiva
13:00 - 14:00 | Lunch Break
14:00 - 18:00 | Practical classes – Lung, gastric and intestinal Organoids Culture and Maintenance
22st November, 2022
9:00 - 10:15 | Methods for Organoid Infection
Carmen Aguilar
10:15 - 11:00 | High content screening using 3D models
André Maia
11:00 - 11:30 | Coffee Break
11:30 - 13:00 | Organs on chips – basic principles
Marta Alves da Silva
13:00 - 14:00 | Lunch Break
14:00 - 18:00 | Practical classes – Lung, gastric and intestinal Organoids Culture and Maintenance
23rd November, 2022
9:00 - 10:15 | Advanced microphysiologic systems
Silvia Mihaila
10:15 - 11:00 | Microfluidics Class
Estrela Neto
11:00 - 11:30 | Coffee Break
11:30 - 12:15 | Microfluidic chips fabrication
Marta Alves da Silva
12:15 - 13:00 | Organoids and 3Rs
Anna Olsson
13:00 - 14:00 | Lunch Break
14:00 - 18:00 | Practical classes – Lung, gastric and intestinal Organoids Culture and Maintenance
Marta Alves da Silva, Filipe Pinto, Clara Bento
24th November, 2022
9:00 - 11:00 | Theoretical/Practical classes – Histology principles and procedures using organoids
Nuno Mendes
11:00 - 11:30 | Coffee Break
11:30 - 13:00 | Maintenance and recovery of organoids from Matrigel
Marta Alves da Silva
13:00 - 14:00 | Lunch Break
14:00 - 18:00 | Practical classes – How to operate microfluidics chips
Maria Azevedo, Paula Sampaio
25th November, 2022
9:00 - 10:30 | Introduction to confocal microscopy of 3D structures
Paula Sampaio
10:30 - 11:00 | Coffee Break
11:00 - 13:00 | Practical classes – Organoids confocal microscopy
Maria Azevedo, Paula Sampaio
13:00 - 14:00 | Lunch Break
14:00 - 16:00 | Practical classes – Lung, gastric and intestinal Organoids Culture and Maintenance
Maria Azevedo, Paula Sampaio
16:00 | Wrap-up and Farewell
Speakers
André Maia, Biosciences Screening, i3S
Anna Olsson, i3S
Carmen Aguilar, University of Wurzburg, Germany
Celso Reis, i3S
Clara Bento, i3S
Cristina Barrias, i3S
Estrela Neto, i3S
Filipe Pinto, i3S
Margarida Saraiva, i3S
Maria Azevedo, Advanced Light Microscopy, i3S
Marta Alves da Silva, i3S
Nuno Mendes, Histology and Electron Microscopy, i3S
Paula Sampaio, Advanced Light Microscopy, i3S
Silvia Mihaila, Utrecht University, The Netherlands
Registration
Registration until 19th of October
Registration fees:
i3S members: 325€
UP members: 375€
External participants - Academy: 400€
External participants - Companies: 500€
Registration fees include:
- i3S members: coffee breaks
- UP members: lunch ticket and coffee breaks
- External participants: lunch ticket and coffee breaks
Certificate of attendance will be issued at the end of the course to participants that meet the following: presence in 90% of the theoretical classes; presence in all practical and theoretical-practical classes.
Please note that your pre-registration does not confirm that you have been selected for the course.
Payment will be requested only after confirmation of acceptance. Details for payment will be sent in the confirmation registration e-mail.
The organizer reserves the right to cancel the course in case of an insufficient number of participants or other unforeseeable events that render the execution of the course.
Cancelation policy:
In case of cancelation, the registration fee will not be refunded.
The organizer reserves the right to cancel the course in case of an insufficient number of participants, organizational reasons, for other unforeseeable events that render the execution of the course impossible. The organizer shall immediately inform the pre-registered participants of such changes.
More Information:
Advanced Training Unit | e-mail: training@i3s.up.pt | tel: +351 226 074 900