Where Ideas Grow

Experimental Design and Analysis of Data from Animal Experiments | 5th Edition

24-26 January 2023 | i3S, Porto
This three-day course aims at making researchers sufficiently knowledgeable on experimental design and statistics to make results from animal experiments more reliable, robust and reproducible, while avoiding animal waste and complying with the 3Rs of animal research, with particular emphasis on ''Reduction'.

Course organizer: Nuno Franco, i3S
Target audience: This course is particularly aimed at researchers responsible for designing and/or carrying out animal experiments. It is also highly recommended for members of Animal Welfare Bodies or Competent Authorities responsible for evaluating animal research projects.
Course duration: 18h
Course language: English
Requirements: A laptop will be necessary to participate in the course, in order to run the statistics software


Poster >>


The course will be held at i3S – Instituto de Investigação e Inovação em Saúde.
Rua Alfredo Allen, 208; 4200-135 Porto, Portugal
Tel: +351 220 408 800 | Site: http://www.i3s.up.pt/ | E-mail: events@i3s.up.pt
GPS coordinates: 41º 10’ 30.008’’ N, 8º 36’ 12.488’’ W.


- Planning for reproducibility in animal research
- Common mistakes in design and  execution of experiments
- Formulating objectives and testing hypotheses
- Avoidance of bias (randomization, blind assessment, adequate controls)
- Formal experimental designs
- Independent replication and appropriate experimental units
- Estimating an adequate sample size
- Basic concepts in probability and statistics
- Single and two factor analysis of variance
- Analysis of covariance
- Data transformations and non-parametric methods
- Multivariate analysis of variance


Derek John Fry | University of Manchester
Pedro Oliveira | ICBAS, University of Porto
Nuno Henrique Franco | i3S, University of Porto
Denisa Mendonça | ICBAS, University of Porto


Limited registrations

Admission criteria: 
- Course participants should have, as a minimum, a degree in the natural or exact sciences.
- Preference will be given to researchers who use - or are about to use - animals for scientific purposes, or are involved in the design and analysis of animal experiments.

Registration fees*:


Early bird

Until 25 November 2022 | Payment deadline: 2 December 2022


26 November 2022 to 9 December 2022 | Payment deadline: 9 December 2022

i3S member / P.CCC Raquel Seruca member

225 €

275 €

External participant

275 €

350 €

* Registration fees include coffee breaks.

A certificate of attendance will be assigned to all participants participating in all lectures and exercises. A final exam will be carried out, and for those passing the exam, a certificate stating the passing of an exam will be issued.

Course pre-registration:

Pre-registration form >>
Please note that your pre-registration does not confirm that you have been selected for the course.

Payment will be requested only after confirmation of acceptance. Details for payment will sent in the confirmation registration e-mail.

Cancelation policy: In case of cancelation, the registration fee will not be refunded.

The organizer reserves the right to cancel the course in case of an insufficient number of participants, organizational reasons, for other unforeseeable events that render the execution of the course impossible. The organizer shall immediately inform the pre-registered participants of such changes.


More Information:

Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 220 408 800