Where Ideas Grow

Course on Optical Microscopy Imaging for Biosciences | 14th edition

20-24 March 2023 | 9:30 to 18:30 | i3S, Porto


The 14th edition of the Course on Optical Microscopy Imaging for Biosciences offers the participants a comprehensive light microscopy course that explores the fundamentals and introduces the state-of-art of advanced fluorescence microscopy techniques. The course will flow from sample preparation to quantitative image analysis. It will include theoretical lectures taught by specialists in the field and hands-on practical modules supported by major microscopy companies where the participants will have the opportunity to work with high-end microscopy technology.

Course Coordination: Paula Sampaio, i3S; Maria Azevedo, i3S


Target audience: The course is designed for Ph.D. students, early career researchers plus all researchers interested in acquiring skills in optical microscopy.
Course duration: 35-40 hours
Course language: English
Certificate: The attendees must attend a minimum of 80% of the course duration

Poster >>


The course will be held at i3S – Instituto de Investigação e Inovação em Saúde.
Rua Alfredo Allen, 208; 4200-135 Porto, Portugal
Tel: +351 220 408 800 | Site: http://www.i3s.up.pt/ | E-mail: events@i3s.up.pt
GPS coordinates: 41º 10’ 30.008’’ N, 8º 36’ 12.488’’ W.



Specimen preparation; 
Light proprieties and image formation; 
Optical contrast techniques in transmission light microscopy; 
Widefield Fluorescence microscopy
Optical sectioning methods: Deconvolution, Laser scanning confocal, two-photon, TIRF and Lightsheet microscopy; 
Live cell imaging; 
Analyzing biomolecular interactions and dynamics: FRET, FLIM, FRAP, etc…; 
Super-resolution microscopy; 
Artificial Intelligence in microscopy;
Quantitative image analysis. 


To see the course program in PDF here. 


Paula Sampaio, i3S
Maria Azevedo, i3S
António Pereira, i3S
Maria Lazaro, i3S
Sylvie Le Guyader, Karolinska Institutet
Rocco D'Antuono, Crick Institute
Simão Coelho, IGC
Alex Turpin, iLoF
Henkjan Gersen, iLoF

More speakers to be announced soon


Registration fee includes coffee breaks, lunches, and course materials. 
Limited number of participants: 20


Early Bird Registration | Until 6th February 2023
(Payment deadline: 8th February 2023)
i3S members / P.CCC Raquel Seruca members - 325€
External Participants - 400€

Late registration | Until 16th February 2023 (Payment deadline: 20th February 2023)
i3S members / P.CCC Raquel Seruca members - 375€
External Participants - 450€


Pre-registration form >>

Please note that your pre-registration does not confirm that you have been selected for the course.

Payment will be requested only after confirmation of acceptance. Details for payment will be sent in the confirmation registration e-mail.

Cancelation policy:

In case of cancelation, the registration fee will not be refunded.

The organizer reserves the right to cancel the course in case of an insufficient number of participants, organizational reasons, or other unforeseeable events that render the execution of the course impossible. The organizer shall immediately inform the pre-registered participants of such changes.









More Information:
Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 220 408 800