Where Ideas Grow

Advanced Course on Organoids Models | 3rd Edition

11-14 April 2023 | i3S, Porto


The main aim of this course is to present the fundamentals of organoid models, specifically intestinal, gastric, and lung models. Participants will learn the basic principles of working with these models, and how to culture and maintain them. Moreover, they will be instructed on how to plan and run experiments using organoids, as well as on methods to analyze experimental data/ results. Microfluidics and organs-on-chip will also be explored. The specific protocols used for imaging, molecular biology, and histology using organoids will be covered.


Target audience: All scientific community
Course approximate duration: 35 hours
Requirements: Participants should bring their own laptop and lab coat.

Prior knowledge required:
- Basic training in Biology, Bioengineering, or related sciences;
- Cell culture techniques knowledge;
- Molecular biology techniques knowledge.

Course organizers: Anna Olsson, i3S; Marta Alves da Silva, i3S

Language: English


Poster >>


Participants will acquire the following skills:
 -  establish and maintain cultures of intestinal, gastric, and lung organoids;
 - learn how to design and plan experiments using organoids;
 -  learn how to choose, set up and work with organs on chips;
 -  prepare organoids samples for histological analysis;
 -  analyze practical outcomes of their cultures by imaging techniques (brightfield and confocal microscopy).



André Maia, i3S
Anna Olsson, i3S
Celso Reis, i3S
Clara Bento, i3S
Cristina Barrias, i3S
Estrela Neto, i3S
Filipe Pinto, i3S
Margarida Saraiva, i3S
Maria Azevedo, Advanced Light Microscopy, i3S
Marta Alves da Silva, i3S
Nuno Mendes, Histology and Electron Microscopy, i3S
Paula Sampaio, Advanced Light Microscopy, i3S
Rossana Correia, Histology and Electron Microscopy, i3S


  Early bird registration:
Until 7th February 2023
Payment deadline: 14th February 2023

Normal registration:
From 8th February to 7th March 2023
Payment deadline: 14th March 2023

i3S members 325 € 400 €
P.CCC Raquel Seruca members 325 € 400 €
UP members 375 € 425 €
External participants - Academy 400 € 475 €
External participants - Companies 500 € 575 €


Registration fees include:
- i3S members and P.CCC Raquel Seruca members: coffee breaks
- UP members: lunch ticket and coffee breaks
- External participants: lunch ticket and coffee breaks


Certificate of attendance will be issued at the end of the course to participants that meet the following: presence in 90% of the theoretical classes; presence in all practical and theoretical-practical classes.

Course pre-registration >>


Please note that your pre-registration does not confirm that you have been selected for the course.
Payment will be requested only after confirmation of acceptance. Details for payment will be sent in the confirmation registration e-mail.
The organizer reserves the right to cancel the course in case of an insufficient number of participants or other unforeseeable events that render the execution of the course.

Cancelation policy
In case of cancellation, the registration fee will not be refunded.
The organizer reserves the right to cancel the course in case of an insufficient number of participants, organizational reasons, or other unforeseeable events that render the execution of the course impossible. The organizer shall immediately inform the pre-registered participants of such changes.


More Information
Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 226 074 900