creating a
healthier future

Hands-on course in transgenesis and mutagenesis in zebrafish – 1st edition

22-26 May 2023 | i3S, Porto


This hands-on course will focus on the training in genetic manipulation of zebrafish, including techniques for transgenesis and mutagenesis. The participants will be offered theoretical and practical sessions on Tol2-mediated transgenesis and Crispr-Cas9 mediated genome editing. Microinjection into zebrafish embryos will be covered, as well as previous preparation of RNA or DNA to inject and subsequent screening to identify positive founder fish.

Course Coordination: José Bessa, i3S; Joana Marques, i3S


Target audience: The course is designed for PhD students and senior researchers 

Course duration: 30 hours

Course language: English 

Certificate: A certificate of attendance will be assigned to participants who attend all lectures.

Admission criteria: Only applicants holding at least a BSc - Bologna 1st cycle degree in Biology, Biochemistry, Veterinary, Medicine, Pharmacy or similar will be admitted.
Note: No previous training in Laboratory animal science is required.

Poster >>


The course will be held at i3S – Instituto de Investigação e Inovação em Saúde.
Rua Alfredo Allen, 208; 4200-135 Porto, Portugal
Tel: +351 220 408 800 | Site: | E-mail:
GPS coordinates: 41º 10’ 30.008’’ N, 8º 36’ 12.488’’ W.


DNA and RNA synthesis and purification for zebrafish microinjection;
Tol2- mediated transgenesis in zebrafish;
Crispr-Cas9 mutagenesis in zebrafish;
Microinjection in one-cell stage zebrafish embryos;
Fluorescence screening of zebrafish embryos;
Genotyping of Crispr-Cas9 induced mutants.


José Bessa, i3S
Joana Marques, i3S
Fábio Ferreira, i3S
Joana Teixeira, i3s
Ana Eufrásio, i3S
Marta Duque, i3S
João Amorim, i3S


Registration fee includes coffee breaks, lunches, and course materials. 
Limited number of participants: 8


Early Bird Registration | Until 13th February 2023 (Payment deadline: 20th February 2023)
i3S members / P.CCC Raquel Seruca members - 200€
UP members – 250€
External Participants - 275€

Standard registration | Until 3rd April 2023 (Payment deadline: 10th April 2023)
i3S members / P.CCC Raquel Seruca members - 225€
UP members – 275€
External Participants - 300€

Pre-registration form >>


Please note that your pre-registration does not confirm that you have been selected for the course.

Payment will be requested only after confirmation of acceptance. Details for payment will be sent in the confirmation registration e-mail.

Cancelation policy:

In case of cancelation, the registration fee will not be refunded.
The organizer reserves the right to cancel the course in case of an insufficient number of participants, organizational reasons, or other unforeseeable events that render the execution of the course impossible. The organizer shall immediately inform the pre-registered participants of such changes.


More Information:

Advanced Training Unit | E-mail: | Tel: +351 226 074 900