Flow Cytometry: From basic knowledge to advanced applications – 2nd edition

18-22 March 2024 | i3S, Porto

Flow cytometry is a fluorescence-based that allows rapid multi-parametric analysis of single cells or particles in suspension. In the last 30 years, flow cytometry has hugely evolved becoming powerful tool prevalent in diverse research fields including immunology, cancer biology and molecular biology. This workshop intends to provide a solid understanding of the principles and applications in Flow Cytometry, combining the theoretical and practical sessions. Applicants may register in basic theoretical training modules, or select the complete training including theoretical and practical sessions.

Course Coordination: Catarina Meireles, i3S; Emília Cardoso, i3S e Alexandre Salvador, Enzifarma

Target audience: The course is designed to Scientists (researchers, students, medical doctors, technicians, engineers) with notions of Flow Cytometry

Course duration: Basic (first 2 days) - 14 hours; Advanced (last 3 days) - 21 hours; Basic + Advanced (5 days) - 35 hours

Course language: English

Certificate: The certificate of participation will be sent to participants who attend the entire duration of the registration category they have selected (Basic; Advanced or Basic+Advanced).

Admission criteria: Pre-registrations will be confirmed and those registered for the Advanced category must have clear notions of the basics in Flow cytometry.

Poster >>

The course will be held at i3S – Instituto de Investigação e Inovação em Saúde.
Rua Alfredo Allen, 208; 4200-135 Porto, Portugal
Tel: +351 220 408 800 | E-mail: events@i3s.up.pt
GPS coordinates: 41º 10’ 30.008’’ N, 8º 36’ 12.488’’ W.

Contents:

• Principles of Flow Cytometry
• Full spectral Flow Cytometry
• Spillover; compensation and Unmixing
• Publishing robust data
• Multicolor Panel Design Importance of Antibody titrations
• Applications: Immunophenotyping; cell cycle and apoptosis
• Single cell Sorting

Practical Topics:

• Flow cytometry analysis in different software
• Hands-on in conventional and Full spectral cytometers
• Hands-on on single cell sorting

Introductory Module

Day 1 (18^th March)

09:00-09:30 Participants Registration

09:30-10:45 Principles of Flow Cytometry (Auditorium Corino de Andrade)
                    Catarina Meirels – i3S

10:45-11:00 Coffee Break

11:00-12:00 Fluorochromes - what about them? (Auditorium Corino de Andrade)
                    Ana Longhini – MSKCC

12:00-13:30 Lunch

13:30-15:00 Introduction to Full Spectrum ProfilingTM technology (Auditorium Corino de Andrade)
                    Ana Vieira – Cytek

15:00-15:15 Coffee Break

15:15-17:00 Spillover; compensations, and Unmixing (Auditorium Corino de Andrade)
                    Rui Gardner – MSKCC

Day 2 (19^th March)

09:15-10:30 Multicolor Panel Design (Auditorium Corino de Andrade)
                    Ana Longhini - MSKCC

10:30-10:45 Coffee Break

10:45-12:00 Standardization Approaches for Immunophenotyping in Clinical Samples (Auditorium Corino de Andrade)
                    Alexandre Salvador – ENZIFarma and Guests

12:00-12:15 Laminar Wash - Next Generation Cell Sample Preparation (Paralab Bio) (Auditorium Corino de Andrade)

12:15-13:45 Lunch

13:45-15:00 Considerations for Planning a Flow Cytometry Experiment and Sample Preparation (Auditorium Corino de Andrade)
                    Mariana Fernandes – iMM

15:00-15:15 Coffee Break

15:15-17:00 Best Practices in Publishing Data (Auditorium Corino de Andrade)
                    Rui Gardner – MSKC

Advanced Module

Day 3 (20^th March)

09:15-10:15 Importance of Single stainings, FMOs and Antibody Titrations (Auditorium Corino de Andrade)
                      Ana Longhini - MSKCC

10:15-10:30 Coffee Break

10:30-11:30 Cell Cycle and Apoptosis (Auditorium Corino de Andrade)
                    Emília Cardisi - i3S

12:00-13:30 Lunch

13:30-16:30 Hands-on:
                   - Titrations: sample preparation
                   - Cytometer hands-on (FACS CANTOII)
                   - Cell Cycle (Accuri C6 Plus)

16:30-17:00 Coffee Break

Day 4 (21^th March)

09:00-10:30 Principles of Cell Sorting (Meeting room C)
                      Rui Gardner - MSKCC

10:30-10:45 Coffee Break

10:45-13:00 Hands-on:
                   - Conventional Flow Cytometry (LRSFortessa)
                   - Panel Design (Pratice)
                   - Laminar Wash (Paralab Bio)

13:00-14:00 Lunch

14:00-15:30 Introduction to FlowJo Analysis (Meeting Room C)
                    Serena di Cecilia – Flowjo

15:30-15h45 Coffee Break

15h45-17:00 Next Steps in Data Analysis (Meeting room C)
                    Serena di Cecilia – Flowjo

Day 5 (22^th March)

09:15-10:00 Q&A (All Speakers) (Auditorium Corino de Andrade)

10:00-10:15 Coffee Break

10:15-12:30 Hands-on:
                   - Full Spectrum Profiling^TM technology (Aurora)
                   - Cell Sorting (FACS ARIAFusion)
                   - FlowJo- Analysis

12:30-14:00 Lunch

14:00-15:30 Introduction to FCSExpress Analysis – Jamie McCarthy - De Novo Software (Meeting Room C)

15:30-15:45 Coffee Break

15:45-16:45 Next Steps in Data Analysis - Jamie McCarthy - De Novo Software (Meeting Room C)

16:45-17:00 Closing Session (Meeting Room C)

Alexandre Salvador, Enzifarma
Ana Longhini, Memorial Sloan Kettering Cancer Center (MSKCC)
Ana Vieira, Cytek (Sponsor)
Jamie McCarthy, De Novo Software (Sponsor)
Catarina Meireles, i3S, Porto PT
Emília Cardoso, i3S, Porto PT
Mariana Fernandes, Instituto de Medicina Molecular João Lobo Antunes (iMM)
Rui Gardner, Memorial Sloan Kettering Cancer Center (MSKCC)
Serena di Cecilia, FlowJo (Sponsor)

Registration fee includes coffee breaks and course materials.

Maximum number of participants – Basic: 40 participants

Maximum number of participants – Advanced: 15 participants - FULL

Fees:

Registration deadline for Basic category until January 31st 2024 (Payment deadline: February 7th 2024)

The registrations for the Advanced and Basic+Advanced categories are full.

Registration form for Basic category >>

Please notice that the number of vacancies for Basic category is limited. Participation will be confirmed on a first-come, first-served basis, upon confirmation of registration fee payment.

Cancelation policy:

In case of cancelation, the registration fee will not be refunded.

The organizer reserves the right to cancel the course in case of an insufficient number of participants, organizational reasons, for other unforeseeable events that render the execution of the course impossible. The organizer shall immediately inform the pre-registered participants of such changes.

More information:

Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 226 074 900