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Visualizing Molecules: A Dive into CryoEM Technology

31 January 2025 | i3S, Porto

Cryo-Electron Microscopy (CryoEM) has emerged as a groundbreaking technique for visualising the structures of biological molecules at near-atomic resolution. The workshop provides an excellent opportunity for participants to gain a comprehensive understanding of the principles and applications of CryoEM.

The workshop will introduce the fundamental principles of CryoEM, including the preparation of biological samples, the formation and reconstruction of images, and the latest applications such as Cryo-CLEM and cellular cryo-tomography. Furthermore, participants will be provided with an overview of the existing infrastructure in Portugal (Cryo-EM PT), offering a unique opportunity to gain insight into integrating CryoEM into their research.

The workshop is suitable for both novice and experienced CryoEM practitioners, offering a comprehensive understanding of CryoEM through practical insights and scientific discussions.

Target: Senior and Postdoctoral researchers, PhD and Master students

Language: English

Organizing Committee: Tatiana Cereija and Paula Sampaio

Poster >>

Venue

The workshop will be held at i3S – Instituto de Investigação e Inovação em Saúde.
Rua Alfredo Allen, 208; 4200-135 Porto, Portugal
Tel: +351 226 074 900 | Site: http://www.i3s.up.pt/ | E-mail: events@i3s.up.pt
GPS coordinates: 41º 10’ 30.008’’ N, 8º 36’ 12.488’’ W.

Program

Preliminary Program

09h25 - 09h30 Welcome

09h30 - 10h20 Introduction to cryoEM: basic principles and state-of-the-art
Oliver Schraidt, INL, Braga

10h20 - 10h35 CryoEM PT infrastructure
Oliver Schraidt, INL, Braga

10h35 - 11h00 Coffee break

11h00 - 11h45 Biological sample preparation for CryoEM
Ana Malheiro, INL, Braga

11h45 - 12h00 Bio break

12h00 - 13h00 Combining cryo-electron tomography and single particle analysis to reveal sperm
molecular diversity and evolution

Tzviya Zeev-Ben-Mordehai, Bijvoet Centre for Biomolecular Research of the Utrecht University, The Netherlands

14h00 - 14h45 CryoEM image formation and basic reconstruction principles
José-María Carazo, Spanish National Center for Biotechnology, CNB-CSIC, Madrid

14h45 - 15h10 Scientific work
Tatiana Cereija, i3S, Porto

15h10 - 15h25 Bio break

15h25 - 16h15 Cryo-Tomography and Cryo-CLEM: From Principles to Cutting-Edge Applications (Online Session)
Moara Lemos, CENABIO, Universidade Federal do Rio de Janeiro, Brazil

16h15 - Final discussion

Speakers

Oliver Schraidt - INL, Braga, Portugal
Ana Malheiro - INL, Braga, Portugal
Tzviya Zeev-Ben-Mordehai - Bijvoet Centre for Biomolecular Research of the Utrecht University, The Netherlands
José-María Carazo - Spanish National Center for Biotechnology, CNB-CSIC, Madrid, Spain
Tatiana Cereija - i3S, Porto, Portugal
Moara Lemos - CENABIO, Universidade Federal do Rio de Janeiro, Brazil

Registration

Limited registrations.
Registration is free but mandatory.

This course is no longer accepting registrations.

Cancellation by the Institution
The i3S reserves the right to cancel the course, in case of an insufficient number of participants, organizational reasons, or other unforeseeable events that render the execution of the course impossible
In addition, i3S reserves the right to amend course times, dates or published prices if absolutely necessary. The organizer shall immediately inform the registered participants of these alterations. Any cancellation or substitution requests should be requested by e-mail.

Sponsors

Information

More information:
Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 226 074 900