Workshop on Genome Editing using CRISPR-Cas9

5-7 June 2019 | i3S - Instituto de Investigação e Inovação em Saúde

The aim of this workshop is to provide hands-on training in genome editing in mammalian cells using the powerful CRISPR/Cas9 system. It will cover topics from sg RNA design, molecular cloning and transfection, through off-target assessment, validation and different applications.

Target Audience:

This workshop is directed for Researcher assistants, PhD students, Post-doc fellows and technicians with a molecular biology background who want to get familiar with genome editing in mammalian cells using CRISPR/Cas9 system or who are already using CRISPR but would like to receive focused training to optimize their experiments. Although no previous experience in genome engineering is required, basic skills on molecular and cell biology will be an advantage.

Course Organizers:

Joana Caldeira, i3S/INEB | Rui Lopes, NIBR| Catarina Gomes, i3S/IPATIMUP

Poster Download

CONTENTS

Theory concepts:

• Nuclease-induced genome editing: NHEJ vs HDR

• Overview of CRISPR-Cas9 systems: nuclease, nickase and catalytic-dead

• Applications of CRISPR-Cas9 for DNA editing (knock-in and knock-out), epigenome editing, gene regulation and imaging

• Generation of in-vivo models

• High-throughput genetic screens

Practical topics:

• Bioinformatic design of guide RNAs and donor templates

• Cloning of guide RNAs into expression vectors

• Transfection of a cell line with Cas9 plasmids and guide RNAs

• Accessing the efficiency of DNA editing

• Specificity of CRISPR reagents (off-target effects, and how to deal with them)

• Validation of clonal cells edited by Cas9

Catarina Gomes, i3S/IPATIMUP, Porto

Eva Brinkman, NKI, The Netherlands

Mario Amendola, Genethon, France

Rui Lopes, NIBR, Switzerland

Registration fee includes admission, course materials and coffee breaks.

Fees:

Until 15th February

i3S members – 200€

UP members – 225€

Academia | non UP members – 250€

Industry – 300€

After 15th February

i3S members – 225€

UP members – 250€

Academia | non UP members – 275€

Industry – 325€

Registration form

Your registration will be confirmed by e-mail after the registration deadline. Payment will be requested after the registration confirmation. The course organizers reserve the right to cancel the course if the participants’ minimum is not guaranteed.

More information:

Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 220 408 800

Workshop on Genome Editing using CRISPR-Cas9

5-7 June, 2019 | i3S - Instituto de Investigação e Inovação em Saúde

The aim of this workshop is to provide hands-on training in genome editing in mammalian cells using the powerful CRISPR/Cas9 system. It will cover topics from sgRNA design, molecular cloning and transfection, through off-target assessment, validation and different applications.

Target Audience:

This workshop is directed for Researcher assistants, PhD students, Post-doc fellows and technicians with a molecular biology background who want to get familiar with genome editing in mammalian cells using CRISPR/Cas9 system or who are already using CRISPR but would like to receive focused training to optimize their experiments.

Although no previous experience in genome engineering is required, basic skills on molecular and cell biology will be an advantage.

Theoretical concepts:

• Nuclease-induced genome editing: NHEJ vs HDR

• Overview of CRISPR-Cas9 systems: nuclease, nickase and catalytic-dead

• Applications of CRISPR-Cas9 for DNA editing (knock-in and knock-out), epigenome editing, gene regulation and imaging

• Generation of in-vivo models

• High-throughput genetic screens

Practical topics:

• Bioinformatic design of guide RNAs and donor templates

• Cloning of guide RNAs into expression vectors

• Transfection of a cell line with Cas9 plasmids and guide RNAs

• Accessing the efficiency of DNA editing

• Specificity of CRISPR reagents (off-target effects, and how to deal with them)

• Validation of clonal cells edited by Cas9

Lecturers:

Catarina Gomes (i3S/IPATIMUP, Porto)

Eva Brinkman (NKI, The Netherlands)

Mario Amendola (Genethon, France)

Rui Lopes (NIBR, Switzerland)

Course Organizers:

Catarina Gomes i3S/IPATIMUP

Joana Caldeira i3S/INEB

Rui Lopes (NIBR)

Registration fee includes admission, course materials and coffee breaks.

Fees:

Until 15th March 2019

i3S members – 225€

UP members – 250€

Academia | non UP members – 275€

Industry – 300€

From 16th March until 15th May 

i3S members – 250€

UP members – 275€

Academia | non UP members – 300€

Industry – 325€

The workshop is full. If you wish to stay in the waiting list, please fill in the following registration form. You will be contacted if there are any cancellation. 

More information:

Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 220 408 800