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Workshop on Genome Editing using CRISPR-Cas9

5-7 June, 2019 | i3S - Instituto de Investigação e Inovação em Saúde

 

The aim of this workshop is to provide hands-on training in genome editing in mammalian cells using the powerful CRISPR/Cas9 system. It will cover topics from sgRNA design, molecular cloning and transfection, through off-target assessment, validation and different applications.

 

Target Audience:

This workshop is directed for Researcher assistants, PhD students, Post-doc fellows and technicians with a molecular biology background who want to get familiar with genome editing in mammalian cells using CRISPR/Cas9 system or who are already using CRISPR but would like to receive focused training to optimize their experiments.

Although no previous experience in genome engineering is required, basic skills on molecular and cell biology will be an advantage.

 

 

 

Contents

Theoretical concepts:

• Nuclease-induced genome editing: NHEJ vs HDR

• Overview of CRISPR-Cas9 systems: nuclease, nickase and catalytic-dead

• Applications of CRISPR-Cas9 for DNA editing (knock-in and knock-out), epigenome editing, gene regulation and imaging

• Generation of in-vivo models

• High-throughput genetic screens

 

Practical topics:

• Bioinformatic design of guide RNAs and donor templates

• Cloning of guide RNAs into expression vectors

• Transfection of a cell line with Cas9 plasmids and guide RNAs

• Accessing the efficiency of DNA editing

• Specificity of CRISPR reagents (off-target effects, and how to deal with them)

• Validation of clonal cells edited by Cas9

 

Speakers

Lecturers:

Catarina Gomes (i3S/IPATIMUP, Porto)

Eva Brinkman (NKI, The Netherlands)

Mario Amendola (Genethon, France)

Rui Lopes (NIBR, Switzerland)

 

Course Organizers:

Catarina Gomes i3S/IPATIMUP

Joana Caldeira i3S/INEB

Rui Lopes (NIBR)

 

Registration

Registration fee includes admission, course materials and coffee breaks.

 

Fees:

Until 15th March 2019

i3S members – 225€

UP members – 250€

Academia | non UP members – 275€

Industry – 300€

 

From 16th March until 15th May 

i3S members – 250€

UP members – 275€

Academia | non UP members – 300€

Industry – 325€

 

The workshop is full. If you wish to stay in the waiting list, please fill in the following registration form. You will be contacted if there are any cancellation. 

 

 

Sponsors

 

More information:

Advanced Training Unit | E-mail: training@i3s.up.pt | Tel: +351 220 408 800