Johnny Lisboa
Fish Immunology & Vaccinology
Junior Researcher
I have a background in biochemistry, having developed specific skills during my PhD in the field of structural biology.
I am currently a researcher at the Fish Immunology and Vaccinology group (i3S, University of Porto), contracted under an FCT-approved project (PTDC\BIA-MIC\29910 \2017) that aims to characterize AB toxins that target antigen-presenting cells and their application as intracellularly antigen delivery vehicles, thus used in vaccination against cancer or intracellular pathogens.
Selected Publications
Lisboa J., Pereira C., Pinto R.D., Rodrigues I.S., Pereira L.M.G., Pinheiro B., Oliveira P., Pereira P.J.B., Azevedo J.E., Durand D., Benz R., do Vale A., dos Santos N.M.S.,
Teixeira A., Loureiro I., Lisboa J., Oliveira P.N., Azevedo J.E., dos Santos N.M.S., do Vale A.,
Freitas I.L., Teixeira A., Loureiro I., Lisboa J., Saraiva A., Dos Santos N.M.S., Do Vale A.,
Lisboa J., Pereira C., Rifflet A., Ayala J., Terceti M.S., Barca A.V., Rodrigues I., Pereira P.J.B., Osorio C.R., Portillo F.G.d., Boneca I.G., Vale A.d., Santos N.M.S.d.,
Ithurbide S., Coste G., Lisboa J., Eugénie N., Bentchikou E., de la Tour C.B., Lig D., Confalonieri F., Sommer S., Quevillon-Cheruel S.C., Servant P.,
Pereira C., Rodrigues I.S., Pereira L.M.G., Lisboa J., Pinto R.D., Araújo L., Oliveira P., Benz R., dos Santos N.M.S., do Vale A.,
Lisboa J., Celma L., Sanchez D., Marquis M., Andreani J., Guérois R., Ochsenbein F., Durand D., Marsin S., Cuniasse P., Radicella J.P., Quevillon-Cheruel S.,
Rodrigues I.S., Pereira L.M.G., Lisboa J., Pereira C., Oliveira P., dos Santos N.M.S., do Vale A.,
Terceti M.S., Vences A., Matanza X.M., Barca A.V., Noia M., Lisboa J., Dos Santos N.M.S., Do Vale A., Osorio C.R.,
Pinheiro J., Lisboa J., Pombinho R., Carvalho F., Carreaux A., Brito C., Pöntinen A., Korkeala H., Dos Santos N.M.S., Morais-Cabral J.H., Sousa S., Cabanes D.,
Devigne A., Guérin P., Lisboa J., Quevillon-Cheruel S., Armengaud J., Sommer S., de la Tour C.B., Servant P.,
Lisboa J., Andreani J., Sanchez D., Boudes M., Collinet B., Liger D., Tilbeurgh H.V., Guérois R., Quevillon-Cheruel S.,
Serre M.C., El Arnaout T., Brooks M.A., Durand D., Lisboa J., Lazar N., Raynal B., van Tilbeurgh H., Quevillon-Cheruel S.,
Durand D., Li De La Sierra-Gallay I., Brooks M.A., Thompson A.W., Lazar N., Lisboa J., Van Tilbeurgh H., Quevillon-Cheruel S.,
Quevillon-Cheruel S., Campo N., Mirouze N., Mortier-Barrière I., Brooks M.A., Boudes M., Durand D., Soulet A.L., Lisboa J., Noirot P., Martin B., Van Tilbeurgh H., Noirot-Gros M.F., Claverys J.P., Polard P.,
Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56. Nature Communications14(1):, 2023. [Journal: Article] [IF: 16,6 (*)]
DOI: 10.1038/s41467-023-43054-z SCOPUS: 85177088681.
DOI: 10.1038/s41467-023-43054-z SCOPUS: 85177088681.
Teixeira A., Loureiro I., Lisboa J., Oliveira P.N., Azevedo J.E., dos Santos N.M.S., do Vale A.,
Characterization and Vaccine Potential of Outer Membrane Vesicles from Photobacteriumαdamselae subsp. piscicida. International Journal of Molecular Sciences24(6):, 2023. [Journal: Article] [CI: 2] [IF: 5,6 (*)]
DOI: 10.3390/ijms24065138 SCOPUS: 85151109789.
DOI: 10.3390/ijms24065138 SCOPUS: 85151109789.
Freitas I.L., Teixeira A., Loureiro I., Lisboa J., Saraiva A., Dos Santos N.M.S., Do Vale A.,
Susceptibility of Sea Bream (Sparus aurata) to AIP56, an AB-Type Toxin Secreted by Photobacterium damselae subsp. piscicida. Toxins14(2):, 2022. [Journal: Article] [CI: 4] [IF: 4,2]
DOI: 10.3390/toxins14020119 SCOPUS: 85124209860.
DOI: 10.3390/toxins14020119 SCOPUS: 85124209860.
Lisboa J., Pereira C., Rifflet A., Ayala J., Terceti M.S., Barca A.V., Rodrigues I., Pereira P.J.B., Osorio C.R., Portillo F.G.d., Boneca I.G., Vale A.d., Santos N.M.S.d.,
A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria. mSphere6(1):1-23, 2021. [Journal: Article] [CI: 3] [IF: 5]
DOI: 10.1128/mSphere.00736-20 SCOPUS: 85101242554.
DOI: 10.1128/mSphere.00736-20 SCOPUS: 85101242554.
Ithurbide S., Coste G., Lisboa J., Eugénie N., Bentchikou E., de la Tour C.B., Lig D., Confalonieri F., Sommer S., Quevillon-Cheruel S.C., Servant P.,
Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins. Frontiers in Microbiology11:, 2020. [Journal: Article] [CI: 9] [IF: 5,6]
DOI: 10.3389/fmicb.2020.01253 SCOPUS: 85087289000.
DOI: 10.3389/fmicb.2020.01253 SCOPUS: 85087289000.
Pereira C., Rodrigues I.S., Pereira L.M.G., Lisboa J., Pinto R.D., Araújo L., Oliveira P., Benz R., dos Santos N.M.S., do Vale A.,
Role of AIP56 disulphide bond and its reduction by cytosolic redox systems for efficient intoxication. Cellular Microbiology22(1):, 2020. [Journal: Article] [CI: 3] [IF: 3,7]
DOI: 10.1111/cmi.13109 SCOPUS: 85073780861.
DOI: 10.1111/cmi.13109 SCOPUS: 85073780861.
Lisboa J., Celma L., Sanchez D., Marquis M., Andreani J., Guérois R., Ochsenbein F., Durand D., Marsin S., Cuniasse P., Radicella J.P., Quevillon-Cheruel S.,
The C-terminal domain of HpDprA is a DNA-binding winged helix domain that does not bind double-stranded DNA. FEBS Journal286(10):1941-1958, 2019. [Journal: Article] [CI: 1] [IF: 4,4]
DOI: 10.1111/febs.14788 SCOPUS: 85062560427.
DOI: 10.1111/febs.14788 SCOPUS: 85062560427.
Rodrigues I.S., Pereira L.M.G., Lisboa J., Pereira C., Oliveira P., dos Santos N.M.S., do Vale A.,
Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida. Scientific Reports9(1):, 2019. [Journal: Article] [CI: 5] [IF: 4]
DOI: 10.1038/s41598-019-45240-w SCOPUS: 85067826636.
DOI: 10.1038/s41598-019-45240-w SCOPUS: 85067826636.
Terceti M.S., Vences A., Matanza X.M., Barca A.V., Noia M., Lisboa J., Dos Santos N.M.S., Do Vale A., Osorio C.R.,
The RstAB system impacts virulence, motility, cell morphology, penicillin tolerance and production of type II secretion system-dependent factors in the fish and human pathogen photobacterium damselae subsp. damselae. Frontiers in Microbiology10(APR):, 2019. [Journal: Article] [CI: 13] [IF: 4,2]
DOI: 10.3389/fmicb.2019.00897 SCOPUS: 85068109857.
DOI: 10.3389/fmicb.2019.00897 SCOPUS: 85068109857.
Pinheiro J., Lisboa J., Pombinho R., Carvalho F., Carreaux A., Brito C., Pöntinen A., Korkeala H., Dos Santos N.M.S., Morais-Cabral J.H., Sousa S., Cabanes D.,
MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence. Nucleic Acids Research46(18):9338-9352, 2018. [Journal: Article] [CI: 24] [IF: 11,1]
DOI: 10.1093/nar/gky624 SCOPUS: 85054896319.
DOI: 10.1093/nar/gky624 SCOPUS: 85054896319.
Devigne A., Guérin P., Lisboa J., Quevillon-Cheruel S., Armengaud J., Sommer S., de la Tour C.B., Servant P.,
PprA protein is involved in chromosome segregation via its physical and functional interaction with DNA gyrase in irradiated Deinococcus radiodurans bacteria. mSphere1(1):, 2016. [Journal: Article] [CI: 17] [IF: 0]
DOI: 10.1128/mSphere.00036-15 SCOPUS: 85019363618.
DOI: 10.1128/mSphere.00036-15 SCOPUS: 85019363618.
Lisboa J., Andreani J., Sanchez D., Boudes M., Collinet B., Liger D., Tilbeurgh H.V., Guérois R., Quevillon-Cheruel S.,
Molecular determinants of the DprA-RecA interaction for nucleation on ssDNA. Nucleic Acids Research42(11):7395-7408, 2014. [Journal: Article] [CI: 20] [IF: 9,1]
DOI: 10.1093/nar/gku349 SCOPUS: 84903144125.
DOI: 10.1093/nar/gku349 SCOPUS: 84903144125.
Serre M.C., El Arnaout T., Brooks M.A., Durand D., Lisboa J., Lazar N., Raynal B., van Tilbeurgh H., Quevillon-Cheruel S.,
The Carboxy-Terminal aN Helix of the Archaeal XerA Tyrosine Recombinase Is a Molecular Switch to Control Site-Specific Recombination. PLoS ONE8(5):, 2013. [Journal: Article] [CI: 16] [IF: 3,5]
DOI: 10.1371/journal.pone.0063010 SCOPUS: 84877110407.
DOI: 10.1371/journal.pone.0063010 SCOPUS: 84877110407.
Durand D., Li De La Sierra-Gallay I., Brooks M.A., Thompson A.W., Lazar N., Lisboa J., Van Tilbeurgh H., Quevillon-Cheruel S.,
Expression, purification and preliminary structural analysis of Escherichia coli MatP in complex with the matS DNA site. Acta Crystallographica Section F: Structural Biology and Crystallization Communications68(6):638-643, 2012. [Journal: Article] [CI: 4] [IF: ,6]
DOI: 10.1107/S1744309112011062 SCOPUS: 84862190152.
DOI: 10.1107/S1744309112011062 SCOPUS: 84862190152.
Quevillon-Cheruel S., Campo N., Mirouze N., Mortier-Barrière I., Brooks M.A., Boudes M., Durand D., Soulet A.L., Lisboa J., Noirot P., Martin B., Van Tilbeurgh H., Noirot-Gros M.F., Claverys J.P., Polard P.,
Structure-function analysis of pneumococcal DprA protein reveals that dimerization is crucial for loading RecA recombinase onto DNA during transformation. Proceedings of the National Academy of Sciences of the United States of America109(37):, 2012. [Journal: Article] [CI: 51] [IF: 9,7]
DOI: 10.1073/pnas.1205638109 SCOPUS: 84866295476.
DOI: 10.1073/pnas.1205638109 SCOPUS: 84866295476.