Johnny Lisboa
Molecular Microbiology
Junior Researcher
I have a background in biochemistry, having developed specific skills during my PhD in the field of structural biology.
I am currently a researcher at the Fish Immunology and Vaccinology group (i3S, University of Porto), contracted under an FCT-approved project (PTDC\BIA-MIC\29910 \2017) that aims to characterize AB toxins that target antigen-presenting cells and their application as intracellularly antigen delivery vehicles, thus used in vaccination against cancer or intracellular pathogens.
Selected Publications
Morais M., Dias F., Figueiredo P., Tavares I., Escudeiro C., Teixeira M.R., Teixeira A., Lisboa J., Mikkonen K.S., Teixeira A.L., Medeiros R.
Silver Nanoparticles (AgNPs) Uptake by Caveolae-Dependent Endocytosis is Responsible for Their Selective Effect Towards Castration Resistant Prostate Cancer. International Journal of Nanomedicine19:9091-9107, 2024. [Journal: Article] [IF: 6.6 (*)]
DOI: 10.2147/IJN.S447645 SCOPUS: 85204065766
Lisboa J., Pereira C., Pinto R.D., Rodrigues I.S., Pereira L.M.G., Pinheiro B., Oliveira P., Pereira P.J.B., Azevedo J.E., Durand D., Benz R., do Vale A., dos Santos N.M.S.
Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56. Nature Communications14(1):, 2023. [Journal: Article] [CI: 1] [IF: 16,6 (*)]
DOI: 10.1038/s41467-023-43054-z SCOPUS: 85177088681
Teixeira A., Loureiro I., Lisboa J., Oliveira P.N., Azevedo J.E., dos Santos N.M.S., do Vale A.
Characterization and Vaccine Potential of Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida. International Journal of Molecular Sciences24(6):, 2023. [Journal: Article] [CI: 4] [IF: 4.9]
DOI: 10.3390/ijms24065138 SCOPUS: 85151109789
Freitas I.L., Teixeira A., Loureiro I., Lisboa J., Saraiva A., Dos Santos N.M.S., Do Vale A.
Susceptibility of Sea Bream (Sparus aurata) to AIP56, an AB-Type Toxin Secreted by Photobacterium damselae subsp. piscicida. Toxins14(2):, 2022. [Journal: Article] [CI: 5] [IF: 4,2]
DOI: 10.3390/toxins14020119 SCOPUS: 85124209860
Lisboa J., Pereira C., Rifflet A., Ayala J., Terceti M.S., Barca A.V., Rodrigues I., Pereira P.J.B., Osorio C.R., Portillo F.G.d., Boneca I.G., Vale A.d., Santos N.M.S.d.
A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria. mSphere6(1):1-23, 2021. [Journal: Article] [CI: 6] [IF: 5]
DOI: 10.1128/mSphere.00736-20 SCOPUS: 85101242554
Ithurbide S., Coste G., Lisboa J., Eugénie N., Bentchikou E., de la Tour C.B., Lig D., Confalonieri F., Sommer S., Quevillon-Cheruel S.C., Servant P.
Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins. Frontiers in Microbiology11:, 2020. [Journal: Article] [CI: 15] [IF: 5,6]
DOI: 10.3389/fmicb.2020.01253 SCOPUS: 85087289000
Pereira C., Rodrigues I.S., Pereira L.M.G., Lisboa J., Pinto R.D., Araújo L., Oliveira P., Benz R., dos Santos N.M.S., do Vale A.
Role of AIP56 disulphide bond and its reduction by cytosolic redox systems for efficient intoxication. Cellular Microbiology22(1):, 2020. [Journal: Article] [CI: 3] [IF: 3,7]
DOI: 10.1111/cmi.13109 SCOPUS: 85073780861
Lisboa J., Celma L., Sanchez D., Marquis M., Andreani J., Guérois R., Ochsenbein F., Durand D., Marsin S., Cuniasse P., Radicella J.P., Quevillon-Cheruel S.
The C-terminal domain of HpDprA is a DNA-binding winged helix domain that does not bind double-stranded DNA. FEBS Journal286(10):1941-1958, 2019. [Journal: Article] [CI: 1] [IF: 4,4]
DOI: 10.1111/febs.14788 SCOPUS: 85062560427
Rodrigues I.S., Pereira L.M.G., Lisboa J., Pereira C., Oliveira P., dos Santos N.M.S., do Vale A.
Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida. Scientific Reports9(1):, 2019. [Journal: Article] [CI: 7] [IF: 4]
DOI: 10.1038/s41598-019-45240-w SCOPUS: 85067826636
Terceti M.S., Vences A., Matanza X.M., Barca A.V., Noia M., Lisboa J., Dos Santos N.M.S., Do Vale A., Osorio C.R.
The RstAB system impacts virulence, motility, cell morphology, penicillin tolerance and production of type II secretion system-dependent factors in the fish and human pathogen photobacterium damselae subsp. damselae. Frontiers in Microbiology10(APR):, 2019. [Journal: Article] [CI: 14] [IF: 4,2]
DOI: 10.3389/fmicb.2019.00897 SCOPUS: 85068109857
Pinheiro J., Lisboa J., Pombinho R., Carvalho F., Carreaux A., Brito C., Pöntinen A., Korkeala H., Dos Santos N.M.S., Morais-Cabral J.H., Sousa S., Cabanes D.
MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence. Nucleic Acids Research46(18):9338-9352, 2018. [Journal: Article] [CI: 29] [IF: 11,1]
DOI: 10.1093/nar/gky624 SCOPUS: 85054896319
Devigne A., Guérin P., Lisboa J., Quevillon-Cheruel S., Armengaud J., Sommer S., de la Tour C.B., Servant P.
PprA protein is involved in chromosome segregation via its physical and functional interaction with DNA gyrase in irradiated Deinococcus radiodurans bacteria. mSphere1(1):, 2016. [Journal: Article] [CI: 18] [IF: 0]
DOI: 10.1128/mSphere.00036-15 SCOPUS: 85019363618
Lisboa J., Andreani J., Sanchez D., Boudes M., Collinet B., Liger D., Tilbeurgh H.V., Guérois R., Quevillon-Cheruel S.
Molecular determinants of the DprA-RecA interaction for nucleation on ssDNA. Nucleic Acids Research42(11):7395-7408, 2014. [Journal: Article] [CI: 20] [IF: 9,1]
DOI: 10.1093/nar/gku349 SCOPUS: 84903144125
Serre M.C., El Arnaout T., Brooks M.A., Durand D., Lisboa J., Lazar N., Raynal B., van Tilbeurgh H., Quevillon-Cheruel S.
The Carboxy-Terminal αN Helix of the Archaeal XerA Tyrosine Recombinase Is a Molecular Switch to Control Site-Specific Recombination. PLoS ONE8(5):, 2013. [Journal: Article] [CI: 16] [IF: 3,5]
DOI: 10.1371/journal.pone.0063010 SCOPUS: 84877110407
Durand D., Li De La Sierra-Gallay I., Brooks M.A., Thompson A.W., Lazar N., Lisboa J., Van Tilbeurgh H., Quevillon-Cheruel S.
Expression, purification and preliminary structural analysis of Escherichia coli MatP in complex with the matS DNA site. Acta Crystallographica Section F: Structural Biology and Crystallization Communications68(6):638-643, 2012. [Journal: Article] [CI: 4] [IF: ,6]
DOI: 10.1107/S1744309112011062 SCOPUS: 84862190152
Quevillon-Cheruel S., Campo N., Mirouze N., Mortier-Barrière I., Brooks M.A., Boudes M., Durand D., Soulet A.L., Lisboa J., Noirot P., Martin B., Van Tilbeurgh H., Noirot-Gros M.F., Claverys J.P., Polard P.
Structure-function analysis of pneumococcal DprA protein reveals that dimerization is crucial for loading RecA recombinase onto DNA during transformation. Proceedings of the National Academy of Sciences of the United States of America109(37):, 2012. [Journal: Article] [CI: 54] [IF: 9,7]
DOI: 10.1073/pnas.1205638109 SCOPUS: 84866295476
Silver Nanoparticles (AgNPs) Uptake by Caveolae-Dependent Endocytosis is Responsible for Their Selective Effect Towards Castration Resistant Prostate Cancer. International Journal of Nanomedicine19:9091-9107, 2024. [Journal: Article] [IF: 6.6 (*)]
DOI: 10.2147/IJN.S447645 SCOPUS: 85204065766
Lisboa J., Pereira C., Pinto R.D., Rodrigues I.S., Pereira L.M.G., Pinheiro B., Oliveira P., Pereira P.J.B., Azevedo J.E., Durand D., Benz R., do Vale A., dos Santos N.M.S.
Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56. Nature Communications14(1):, 2023. [Journal: Article] [CI: 1] [IF: 16,6 (*)]
DOI: 10.1038/s41467-023-43054-z SCOPUS: 85177088681
Teixeira A., Loureiro I., Lisboa J., Oliveira P.N., Azevedo J.E., dos Santos N.M.S., do Vale A.
Characterization and Vaccine Potential of Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida. International Journal of Molecular Sciences24(6):, 2023. [Journal: Article] [CI: 4] [IF: 4.9]
DOI: 10.3390/ijms24065138 SCOPUS: 85151109789
Freitas I.L., Teixeira A., Loureiro I., Lisboa J., Saraiva A., Dos Santos N.M.S., Do Vale A.
Susceptibility of Sea Bream (Sparus aurata) to AIP56, an AB-Type Toxin Secreted by Photobacterium damselae subsp. piscicida. Toxins14(2):, 2022. [Journal: Article] [CI: 5] [IF: 4,2]
DOI: 10.3390/toxins14020119 SCOPUS: 85124209860
Lisboa J., Pereira C., Rifflet A., Ayala J., Terceti M.S., Barca A.V., Rodrigues I., Pereira P.J.B., Osorio C.R., Portillo F.G.d., Boneca I.G., Vale A.d., Santos N.M.S.d.
A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria. mSphere6(1):1-23, 2021. [Journal: Article] [CI: 6] [IF: 5]
DOI: 10.1128/mSphere.00736-20 SCOPUS: 85101242554
Ithurbide S., Coste G., Lisboa J., Eugénie N., Bentchikou E., de la Tour C.B., Lig D., Confalonieri F., Sommer S., Quevillon-Cheruel S.C., Servant P.
Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins. Frontiers in Microbiology11:, 2020. [Journal: Article] [CI: 15] [IF: 5,6]
DOI: 10.3389/fmicb.2020.01253 SCOPUS: 85087289000
Pereira C., Rodrigues I.S., Pereira L.M.G., Lisboa J., Pinto R.D., Araújo L., Oliveira P., Benz R., dos Santos N.M.S., do Vale A.
Role of AIP56 disulphide bond and its reduction by cytosolic redox systems for efficient intoxication. Cellular Microbiology22(1):, 2020. [Journal: Article] [CI: 3] [IF: 3,7]
DOI: 10.1111/cmi.13109 SCOPUS: 85073780861
Lisboa J., Celma L., Sanchez D., Marquis M., Andreani J., Guérois R., Ochsenbein F., Durand D., Marsin S., Cuniasse P., Radicella J.P., Quevillon-Cheruel S.
The C-terminal domain of HpDprA is a DNA-binding winged helix domain that does not bind double-stranded DNA. FEBS Journal286(10):1941-1958, 2019. [Journal: Article] [CI: 1] [IF: 4,4]
DOI: 10.1111/febs.14788 SCOPUS: 85062560427
Rodrigues I.S., Pereira L.M.G., Lisboa J., Pereira C., Oliveira P., dos Santos N.M.S., do Vale A.
Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida. Scientific Reports9(1):, 2019. [Journal: Article] [CI: 7] [IF: 4]
DOI: 10.1038/s41598-019-45240-w SCOPUS: 85067826636
Terceti M.S., Vences A., Matanza X.M., Barca A.V., Noia M., Lisboa J., Dos Santos N.M.S., Do Vale A., Osorio C.R.
The RstAB system impacts virulence, motility, cell morphology, penicillin tolerance and production of type II secretion system-dependent factors in the fish and human pathogen photobacterium damselae subsp. damselae. Frontiers in Microbiology10(APR):, 2019. [Journal: Article] [CI: 14] [IF: 4,2]
DOI: 10.3389/fmicb.2019.00897 SCOPUS: 85068109857
Pinheiro J., Lisboa J., Pombinho R., Carvalho F., Carreaux A., Brito C., Pöntinen A., Korkeala H., Dos Santos N.M.S., Morais-Cabral J.H., Sousa S., Cabanes D.
MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence. Nucleic Acids Research46(18):9338-9352, 2018. [Journal: Article] [CI: 29] [IF: 11,1]
DOI: 10.1093/nar/gky624 SCOPUS: 85054896319
Devigne A., Guérin P., Lisboa J., Quevillon-Cheruel S., Armengaud J., Sommer S., de la Tour C.B., Servant P.
PprA protein is involved in chromosome segregation via its physical and functional interaction with DNA gyrase in irradiated Deinococcus radiodurans bacteria. mSphere1(1):, 2016. [Journal: Article] [CI: 18] [IF: 0]
DOI: 10.1128/mSphere.00036-15 SCOPUS: 85019363618
Lisboa J., Andreani J., Sanchez D., Boudes M., Collinet B., Liger D., Tilbeurgh H.V., Guérois R., Quevillon-Cheruel S.
Molecular determinants of the DprA-RecA interaction for nucleation on ssDNA. Nucleic Acids Research42(11):7395-7408, 2014. [Journal: Article] [CI: 20] [IF: 9,1]
DOI: 10.1093/nar/gku349 SCOPUS: 84903144125
Serre M.C., El Arnaout T., Brooks M.A., Durand D., Lisboa J., Lazar N., Raynal B., van Tilbeurgh H., Quevillon-Cheruel S.
The Carboxy-Terminal αN Helix of the Archaeal XerA Tyrosine Recombinase Is a Molecular Switch to Control Site-Specific Recombination. PLoS ONE8(5):, 2013. [Journal: Article] [CI: 16] [IF: 3,5]
DOI: 10.1371/journal.pone.0063010 SCOPUS: 84877110407
Durand D., Li De La Sierra-Gallay I., Brooks M.A., Thompson A.W., Lazar N., Lisboa J., Van Tilbeurgh H., Quevillon-Cheruel S.
Expression, purification and preliminary structural analysis of Escherichia coli MatP in complex with the matS DNA site. Acta Crystallographica Section F: Structural Biology and Crystallization Communications68(6):638-643, 2012. [Journal: Article] [CI: 4] [IF: ,6]
DOI: 10.1107/S1744309112011062 SCOPUS: 84862190152
Quevillon-Cheruel S., Campo N., Mirouze N., Mortier-Barrière I., Brooks M.A., Boudes M., Durand D., Soulet A.L., Lisboa J., Noirot P., Martin B., Van Tilbeurgh H., Noirot-Gros M.F., Claverys J.P., Polard P.
Structure-function analysis of pneumococcal DprA protein reveals that dimerization is crucial for loading RecA recombinase onto DNA during transformation. Proceedings of the National Academy of Sciences of the United States of America109(37):, 2012. [Journal: Article] [CI: 54] [IF: 9,7]
DOI: 10.1073/pnas.1205638109 SCOPUS: 84866295476