Where Ideas Grow

Advanced Light Microscopy

Advanced Light Microscopy (ALM) is the i3S scientific platform specialising in state-of-the-art optical microscopy for the study of cellular systems in life science research areas such as cell biology, oncobiology, neurobiology, immunology, and biomaterials. The ALM provides open access to high-end imaging technology and extensive expertise in advanced optical microscopy applied to multi-dimensional imaging of cells and tissues, live cell imaging, functional and molecular imaging (e.g. FRAP, FRET, FLIM), and bio-image data analysis. Available equipment includes transmission/widefield fluorescence microscopes (one with TIRF), spinning disc microscopes (one with SR-SIM), laser scanning confocal microscopes (one with STED, FLIM, and FCS), and a light sheet microscope. Workstations with bio-image analysis software are also available.

The scientific platform provides scientific advice on experimental design, training in the practical use of the equipment and image analysis, and scientific advice on the development of research projects. It also aims to increase knowledge in bioimaging by organising several post-graduate courses in light microscopy and image analysis for the scientific and industrial communities.

The ALM is a core facility of the national research infrastructure PPBI-Portuguese Platform of BioImaging and of the European research infrastructure Euro-Bioimaging ERIC.

Platform Head



  • Open-access to light microscopy equipment
  • Open-access to workstations and specialized software for bioimage analysis
  • Scientific advisement in experiment planning
  • Advisement and support in bioimage analysis
  • Hands-on training on equipment operation



Fees apply for use of ALM resources (price information soon available)


PPBI – Portuguese Platform of BioImage


ELMI - European Light Microscopy Iniciative


NEUBIAS – Network of European BioImage Analysts (COST action CA15124)


COMULIS - Correlated Multimodal Imaging in Life Sciences (COST action CA17121)