Advanced Light Microscopy
Advanced Light Microscopy (ALM) is the i3S scientific platform specialising in state-of-the-art optical microscopy for the study of cellular systems in life science research areas such as cell biology, oncobiology, neurobiology, immunology, and biomaterials. The ALM provides open access to high-end imaging technology and extensive expertise in advanced optical microscopy applied to multi-dimensional imaging of cells and tissues, live cell imaging, functional and molecular imaging (e.g. FRAP, FRET, FLIM), and bio-image data analysis. Available equipment includes transmission/widefield fluorescence microscopes (one with TIRF), spinning disc microscopes (one with SR-SIM), laser scanning confocal microscopes (one with STED, FLIM, and FCS), and a light sheet microscope. Workstations with bio-image analysis software are also available.
The scientific platform provides scientific advice on experimental design, training in the practical use of the equipment and image analysis, and scientific advice on the development of research projects. It also aims to increase knowledge in bioimaging by organising several post-graduate courses in light microscopy and image analysis for the scientific and industrial communities.
The ALM is a core facility of the national research infrastructure PPBI-Portuguese Platform of BioImaging and of the European research infrastructure Euro-Bioimaging ERIC.
Platform Head
Team
Resources
Laser Point Scanning Confocal System
Leica STELLARIS 8 STED FALCON
Leica TCS SP8
Leica TCS SP5
Multiphoton System
Bruker Ultima 2Pplus (Room -200.S2)
Spinning Disk Confocal Systems
Nikon Crest X-Light V3 DeepSIM
Andor Revolution XD
Andor BC43
Widefield upright microscopes
Zeiss Axio Imager Z1 #1
Zeiss Axio Imager Z1 #2
Widefield inverted microscopes
Leica DMI6000 FFW
Leica DMI6000 Timelapse
Nikon ECLIPSE Ti TIRF
Light-sheet Fluorescence microscopes
Digital Scanning Lightsheet Fluorescence Microscope (dSLFM)
LEMOLish Lightsheet Fluorescence Microscope
Image analysis software
Huygens (Nyquist rate calculator)
Services
- Open-access to light microscopy equipment
- Open-access to workstations and specialized software for bioimage analysis
- Scientific advisement in experiment planning
- Advisement and support in bioimage analysis
- Hands-on training on equipment operation
Fees apply for use of ALM resources (price information soon available)
Partnerships
PPBI – Portuguese Platform of BioImage
ELMI - European Light Microscopy Iniciative
NEUBIAS – Network of European BioImage Analysts (COST action CA15124)
COMULIS - Correlated Multimodal Imaging in Life Sciences (COST action CA17121)