Daniela Freitas
Glycobiology in Cancer
Junior Researcher
Daniela Freitas is a biochemist who received a MSc degree in Clinical Biochemistry by the University of Aveiro working in tumor drug resistance mediated by cancer stem cells. Daniela received her PhD in Cellular and Molecular Biotechnology Applied to Health Sciences by the University of Porto in 2019. Daniela‘s work has been focused on understanding how tumor-associated glycosylation impacts cancer progression and patients' poor survival. She uncover the role of specific glycans (carbohydrates/sugars) in mediating gastric cancer aggressiveness associated with patients ‘poor survival. Furthermore, Daniela has combined her expertise in glycobiology in cancer to open new avenues in the study of cancer extracellular vesicles (EVs). Daniela reported for the first time the presence of the cancer-associated glycan, sialyl-Tn in cancer EVs, opening new avenues for cancer biomarker discovery. Daniela has also received two grants from the American Portuguese Biomedical Research Fund to pursuit her research in the EV field at Weill Cornell Medicine, New York City, USA, where she participated in different projects that identified for the first time the secretion of novel particles, called exomeres, with relevance in cancer. Daniela has also published a main statement in the EV field where she describes the best EV isolation trategy for EV glycosylation downstream analysis. After returning to Portugal, Daniela started a post-Doc position at I3S to research the role of EV glycosylation in intercellular communication and cancer progression for novel cancer biomarker discovery and therapeutic strategies.
Selected Publications
Freitas D., Balmaña M., Poças J., Campos D., Osório H., Konstantinidi A., Vakhrushev S.Y., Magalhães A., Reis C.A.,
Freitas D., Campos D., Gomes J., Pinto F., Macedo J.A., Matos R., Mereiter S., Pinto M.T., Polónia A., Gartner F., Magalhães A., Reis C.A.,
Zhang H., Freitas D., Kim H.S., Fabijanic K., Li Z., Chen H., Mark M.T., Molina H., Martin A.B., Bojmar L., Fang J., Rampersaud S., Hoshino A., Matei I., Kenific C.M., Nakajima M., Mutvei A.P., Sansone P., Buehring W., Wang H., Jimenez J.P., Cohen-Gould L., Paknejad N., Brendel M., Manova-Todorova K., Magalhães A., Ferreira J.A., Osório H., Silva A.M., Massey A., Cubillos-Ruiz J.R., Galletti G., Giannakakou P., Cuervo A.M., Blenis J., Schwartz R., Brady M.S., Peinado H., Bromberg J., Matsui H., Reis C.A., Lyden D.,
Rodrigues G., Hoshino A., Kenific C.M., Matei I.R., Steiner L., Freitas D., Kim H.S., Oxley P.R., Scandariato I., Casanova-Salas I., Dai J., Badwe C.R., Gril B., Tešic Mark M., Dill B.D., Molina H., Zhang H., Benito-Martin A., Bojmar L., Ararso Y., Offer K., LaPlant Q., Buehring W., Wang H., Jiang X., Lu T.M., Liu Y., Sabari J.K., Shin S.J., Narula N., Ginter P.S., Rajasekhar V.K., Healey J.H., Meylan E., Costa-Silva B., Wang S.E., Rafii S., Altorki N.K., Rudin C.M., Jones D.R., Steeg P.S., Peinado H., Ghajar C.M., Bromberg J., de Sousa M., Pisapia D., Lyden D.,
Campos D., Freitas D., Gomes J., Magalhães A., Steentoft C., Gomes C., Vester-Christensen M.B., Ferreira J.A., Afonso L.P., Santos L.L., De Sousa J.P., Mandel U., Clausen H., Vakhrushev S.Y., Reis C.A.,
Magalhães A., Marcos-Pinto R., Nairn A.V., dela Rosa M., Ferreira R.M., Junqueira-Neto S., Freitas D., Gomes J., Oliveira P., Santos M.R., Marcos N.T., Xiaogang W., Figueiredo C., Oliveira C., Dinis-Ribeiro M., Carneiro F., Moremen K.W., David L., Reis C.A.,
Amorim S., da Costa D.S., Freitas D., Reis C.A., Reis R.L., Pashkuleva I., Pires R.A.,
Amorim I., Freitas D.P., Magalhães A., Faria F., Lopes C., Faustino A.M., Smet A., Haesebrouck F., Reis C.A., Gärtner F.,
Campos D., Freitas D., Gomes J., Reis C.A.,
Duarte H.O., Freitas D., Gomes C., Gomes J., Magalhães A., Reis C.A.,
Freitas D.P., Teixeira C.A., Santos-Silva F., Vasconcelos M.H., Almeida G.M.,
Different isolation approaches lead to diverse glycosylated extracellular vesicle populations. Journal of Extracellular Vesicles8(1):, 2019. [Journal: Article] [CI: 71] [IF: 15]
DOI: 10.1080/20013078.2019.1621131 SCOPUS: 85074725417.
DOI: 10.1080/20013078.2019.1621131 SCOPUS: 85074725417.
Freitas D., Campos D., Gomes J., Pinto F., Macedo J.A., Matos R., Mereiter S., Pinto M.T., Polónia A., Gartner F., Magalhães A., Reis C.A.,
O-glycans truncation modulates gastric cancer cell signaling and transcription leading to a more aggressive phenotype. EBioMedicine40:349-362, 2019. [Journal: Article] [CI: 57] [IF: 5,7]
DOI: 10.1016/j.ebiom.2019.01.017 SCOPUS: 85060115866.
DOI: 10.1016/j.ebiom.2019.01.017 SCOPUS: 85060115866.
Zhang H., Freitas D., Kim H.S., Fabijanic K., Li Z., Chen H., Mark M.T., Molina H., Martin A.B., Bojmar L., Fang J., Rampersaud S., Hoshino A., Matei I., Kenific C.M., Nakajima M., Mutvei A.P., Sansone P., Buehring W., Wang H., Jimenez J.P., Cohen-Gould L., Paknejad N., Brendel M., Manova-Todorova K., Magalhães A., Ferreira J.A., Osório H., Silva A.M., Massey A., Cubillos-Ruiz J.R., Galletti G., Giannakakou P., Cuervo A.M., Blenis J., Schwartz R., Brady M.S., Peinado H., Bromberg J., Matsui H., Reis C.A., Lyden D.,
Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation. Nature Cell Biology20(3):332-343, 2018. [Journal: Article] [CI: 1010] [IF: 17,7]
DOI: 10.1038/s41556-018-0040-4 SCOPUS: 85042193942.
DOI: 10.1038/s41556-018-0040-4 SCOPUS: 85042193942.
Rodrigues G., Hoshino A., Kenific C.M., Matei I.R., Steiner L., Freitas D., Kim H.S., Oxley P.R., Scandariato I., Casanova-Salas I., Dai J., Badwe C.R., Gril B., Tešic Mark M., Dill B.D., Molina H., Zhang H., Benito-Martin A., Bojmar L., Ararso Y., Offer K., LaPlant Q., Buehring W., Wang H., Jiang X., Lu T.M., Liu Y., Sabari J.K., Shin S.J., Narula N., Ginter P.S., Rajasekhar V.K., Healey J.H., Meylan E., Costa-Silva B., Wang S.E., Rafii S., Altorki N.K., Rudin C.M., Jones D.R., Steeg P.S., Peinado H., Ghajar C.M., Bromberg J., de Sousa M., Pisapia D., Lyden D.,
Tumour exosomal CEMIP protein promotes cancer cell colonization in brain metastasis. Nature Cell Biology21(11):1403-1412, 2019. [Journal: Article] [CI: 231] [IF: 20]
DOI: 10.1038/s41556-019-0404-4 SCOPUS: 85074784808.
DOI: 10.1038/s41556-019-0404-4 SCOPUS: 85074784808.
Campos D., Freitas D., Gomes J., Magalhães A., Steentoft C., Gomes C., Vester-Christensen M.B., Ferreira J.A., Afonso L.P., Santos L.L., De Sousa J.P., Mandel U., Clausen H., Vakhrushev S.Y., Reis C.A.,
Probing the O-glycoproteome of gastric cancer cell lines for biomarker discovery. Molecular and Cellular Proteomics14(6):1616-1629, 2015. [Journal: Article] [CI: 87] [IF: 5,9]
DOI: 10.1074/mcp.M114.046862 SCOPUS: 84930470850.
DOI: 10.1074/mcp.M114.046862 SCOPUS: 84930470850.
Magalhães A., Marcos-Pinto R., Nairn A.V., dela Rosa M., Ferreira R.M., Junqueira-Neto S., Freitas D., Gomes J., Oliveira P., Santos M.R., Marcos N.T., Xiaogang W., Figueiredo C., Oliveira C., Dinis-Ribeiro M., Carneiro F., Moremen K.W., David L., Reis C.A.,
Helicobacter pylori chronic infection and mucosal inflammation switches the human gastric glycosylation pathways. Biochimica et Biophysica Acta - Molecular Basis of Disease1852(9):1928-1939, 2015. [Journal: Article] [CI: 63] [IF: 5,2]
DOI: 10.1016/j.bbadis.2015.07.001 SCOPUS: 84940455366.
DOI: 10.1016/j.bbadis.2015.07.001 SCOPUS: 84940455366.
Amorim S., da Costa D.S., Freitas D., Reis C.A., Reis R.L., Pashkuleva I., Pires R.A.,
Molecular weight of surface immobilized hyaluronic acid influences CD44-mediated binding of gastric cancer cells. Scientific Reports8(1):, 2018. [Journal: Article] [CI: 43] [IF: 4]
DOI: 10.1038/s41598-018-34445-0 SCOPUS: 85055618605.
DOI: 10.1038/s41598-018-34445-0 SCOPUS: 85055618605.
Amorim I., Freitas D.P., Magalhães A., Faria F., Lopes C., Faustino A.M., Smet A., Haesebrouck F., Reis C.A., Gärtner F.,
A comparison of Helicobacter pylori and non-Helicobacter pylori Helicobacter spp. Binding to Canine Gastric Mucosa with Defined Gastric Glycophenotype. Helicobacter19(4):249-259, 2014. [Journal: Article] [CI: 14] [IF: 4,1]
DOI: 10.1111/hel.12125 SCOPUS: 84904629577.
DOI: 10.1111/hel.12125 SCOPUS: 84904629577.
Campos D., Freitas D., Gomes J., Reis C.A.,
Glycoengineered cell models for the characterization of cancer O-glycoproteome: An innovative strategy for biomarker discovery. Expert Review of Proteomics12(4):337-342, 2015. [Journal: Article] [CI: 10] [IF: 3,5]
DOI: 10.1586/14789450.2015.1059758 SCOPUS: 84937207849.
DOI: 10.1586/14789450.2015.1059758 SCOPUS: 84937207849.
Duarte H.O., Freitas D., Gomes C., Gomes J., Magalhães A., Reis C.A.,
Mucin-type O-glycosylation in gastric carcinogenesis. Biomolecules6(3):, 2016. [Journal: Review] [CI: 38]
DOI: 10.3390/biom6030033 SCOPUS: 85012048870.
DOI: 10.3390/biom6030033 SCOPUS: 85012048870.
Freitas D.P., Teixeira C.A., Santos-Silva F., Vasconcelos M.H., Almeida G.M.,
Therapy-induced enrichment of putative lung cancer stem-like cells. International Journal of Cancer134(6):1270-1278, 2014. [Journal: Article] [CI: 52] [IF: 5,1]
DOI: 10.1002/ijc.28478 SCOPUS: 84891825186.
DOI: 10.1002/ijc.28478 SCOPUS: 84891825186.