Where Ideas Grow


The Bioimaging Scientific Platform was created in 2010 following INEB’s successful application to structural funds of the ON2 program (2007-2013 North Regional Operational Program; SAIECT-IEC/2/2010). Our focus includes the fields of Biomedicine, Biomaterials, Nanomedicine, and Regenerative Medicine. We are involved in developing, improving, integrating, and using bioimaging solutions through research, technology development, training, and education.

Imaging technologies available include ex vivo, in vitro, and in vivo applications, from the molecule to the organism (small experimentation animals), for chemical, functional, and morphological characterization.

Training is provided to all users and courses are organized for the whole scientific community.

Team: María Lázaro – Head | Dalila Pedro – Team Member | Ana Paula Pêgo – Scientific Coordinator

Contacts: b.image@ineb.up.pt

                 maria.glazaro@i3s.up.pt | Lab 004S4 (Ext 6058)

                 dalila.pedro@i3s.up.pt | Lab 004S4 (Ext 6058)


The Bioimaging Scientific Platform is part of the Portuguese Platform of Bioimaging (PPBI). The PPBI got a grant from the FCT National Roadmap of Research Infrastructures that allowed to hire human resources and acquire new equipment. We kindly ask you that when you have used resources from our Scientific Platform please add in the acknowledgments of your papers and thesis (MSc and PhD) the reference of this project (PPBI-POCI-01-0145-FEDER-022122). This is mandatory from FCT project rules.

With this, you are contributing to the success of future funding for human resources and new equipment.

As a suggestion, you may use one of the following sentences:

"The authors acknowledge the support of the i3S Scientific Platform Bioimaging, a member of the national infrastructure PPBI - Portuguese Platform of Bioimaging (PPBI-POCI-01-0145-FEDER-022122)."


"The authors acknowledge the support of the i3S Scientific Platform Bioimaging a member of the PPBI (PPBI-POCI-01-0145-FEDER-022122)."

Please also send us a copy or reference of the published work (article, thesis, other,...).

Platform Head



The collection of resources available allows for the imaging/characterization from molecules to organisms (small experimentation animals). Technologies available and example applications include:

  • Optical microscopy provides multidimensional functional and structural information of a sample in a non-invasive manner. With our widefield and confocal microscopes applications include multimodal imaging of biological samples; acquisition of a series of overlapping tiled images acquired over a defined area; live cell imaging; FRAP; and FRET amongst others.
  • Imaging flow cytometry constitutes a powerful combination between flow cytometry and microscopy, associating statistical strength with imaging at high acquisition speed. Example applications of this technology include localization and colocalization analysis; cell cycle and mitosis; morphological changes; and spot count amongst others.
  • Raman and FTIR spectroscopy are vibrational spectroscopy techniques that obtain information on the functional groups of the molecules allowing for the chemical characterization of samples in a non-destructive manner and without sample preprocessing. These technologies provide information about molecular vibrations useful for sample identification (fingerprint) and quantitation. With our confocal Raman/FTIR microscope we can also obtain chemical images of the samples (mapping), which are useful to analyze distributions. Example applications include: chemical characterization of biological and synthetic samples; compound distribution within a sample; disease detection; drug design; etc 
  • Micro-computed tomography is a nondestructive and noninvasive technique requiring no sample preparation that uses low-dose X-ray imaging and computed tomography to generate 3D image datasets. It is possible then to analyze the morphology and internal microstructure of small animals or samples with resolution down to the micrometric level. Frequently used for imaging bone tissue, this equipment also allows for the image acquisition of body fat and lungs. With the use of contrast agents, its potential use expands to the imaging of soft tissues and the vasculature. Our stand-alone micro-CT can deliver high-quality images at an X-ray dose low enough to enable longitudinal true micro-CT preclinical studies. Thus it allows monitoring of disease progression throughout the complete study, and, as a result, a reduction of the number of animals used per experiment. Example applications include characterization of bone morphometrics; tumor and blood vessel imaging; cardiovascular phenotyping; imaging of body fat and lungs; etc.
  • Microultrasounds is a real-time imaging technology based on the transmission and reception of ultrasound waves, which is capable of generating high-resolution images for true longitudinal studies in animals. We can obtain in vivo anatomical, functional, physiological, and molecular data simultaneously, in real-time, and with a resolution down to 30 µm, allowing for the visualization of vasculature. Example applications of the Vevo 2100 include cardiovascular phenotyping; image-guided cardiac injection; pregnancy research; tumor volume quantification; and bladder imaging amongst others.

More information on each system configuration can be checked in the corresponding Resources area.


Equipment for in vivo applications
  • MicroCT SkyScan1276 (Bruker)

The SkyScan1276 operates with an X-ray source within the energy range from 20-100 kV. Available filters include Al 0.25 mm, Al 0.5 mm, Al Cu 0.03 mm, and a dose-reducing filter. The equipment has the possibility to reach a minimum pixel size of 2.84 µm (only for ex vivo applications). The scanning diameter sizes range from 12 to 80 mm depending on magnification. Supports for sample holding include three animal beds for young mice, adult mice and young rats, and for adult rats (not suitable for old or obese rats); and three supports for ex vivo samples. A volatile-anesthesia system is available with the equipment for the in vivo applications (just compatible with the three animal beds). For in vivo imaging with animal beds, ECG carbon electrodes are available.

If contrast agents are needed, they must be supplied by the user.

Person responsible: María Lázaro  | maria.glazaro@i3s.up.pt

More information about standard operating procedures and technical information can be found in the Documents section.

  • micro-ultrasounds Vevo 2100 (230 V)

Our system allows working in M-mode, PW Doppler Mode and Color Doppler Mode. Image analysis for physiological trace including ECG, respiration waveform and body temperature.

The Vevo2100 is equipped with a MS400: 38Hz Microscan transducer with a broadband frequency: 18 MHz – 38MHz. Capable of working in M-mode, PW Doppler Mode and Color Doppler Mode. Image station plus imaging station extension with injection mount. Also the software package can be used for B-mode (2D) image capture and analysis, Cineloop image review, capture and display with integrated physiolical trace including ECG, respiration waveform and body temperature.

Person responsible: Sofia Lamas  | sofia.lamas@i3s.up.pt

Technical information can be found in the Documents section.

Equipment for in vitro and ex vivo applications
  • Confocal Raman/FTIR Microscope, LabRAM HR800 UV, Horiba Jobin-Yvon

Our system allows for spectra collection in 2D. Thanks to the confocal setup, detailed images and analysis can be obtained and fluorescence interference can be reduced.
Configuration: lasers: 325, 515.5, 633, and 785 nm; detector: CCD air cooled; spectrograph with gratings: 600, 1800, and 2400 l/mm and software for data acquisition and analysis: LabSpec 5. Objectives for Raman spectroscopy include 10X, 50X, and 100X. Objectives for FTIR: 10X glass, 15X ARO and 36X ATR

Person responsible: María Lázaro | maria.glazaro@i3s.up.pt

More information about standard operating procedures and technical information can be found in the Documents section.

  • Confocal Laser Scanning Microscope Leica TCS-SP5 AOBS, Leica microsystems

Our system allows 3D imaging, colocalization, excitation fingerprinting, FRET, FRAP and FLIP, photo-switching, and photo-activation. Live cell imaging experiments can also be performed (controlled temperature and CO2).

Configuration: lasers available: 405, 488, 561, 594, and 633 nm; objectives: 10X dry, 20X immersion, 40X, and 63X oil and 63X glycerol; adapters for 35 mm in diameter plates, well plates, and 25 mm in diameter coverslips; 3 detectors: 1 Hybrid detector and 2 PMTs; motorized stage.

A dedicated offline workstation for data analysis is available.

Person responsible: María Lázaro (maria.glazaro@i3s.up.pt)

More information about standard operating procedures and technical information can be found in the Documents section.

  • Imaging flow cytometer (ImageStreamX,Cytek)

The ImageStreamX is equipped with a 488 nm for the excitation of fluorophores and a 785 nm laser for side-scatter imaging. Objective available: 40X (image pixel size 0.5 µm).

The table of suggested fluorochromes for being used in our ImageStreamX can be seen here.

A dedicated offline workstation for data analysis is available.

Person responsible: María Lázaro | maria.glazaro@i3s.up.pt

More information about standard operating procedures and technical information can be found in the Documents section.

  • Widefield microscope Axio Observer Z1 (Carl Zeiss)

Inverted fluorescence microscopes are capable of producing fluorescence illumination through an episcopic and optical pathway. The Observer Z1 microscope allows images using brightfield, differential interference contrast (DIC), phase contrast, and widefield fluorescence techniques. Our system is equipped with a motorized stage and, with the Zen Blue software, is equipped also with the modules: Timelapse, Z-Stack, Mark&Find, MosaiX.

Configuration: fluorescence filters for 365, 470, 565, and 640 nm; objectives: 5X air, 10X air, 20X air, LD, 40X air, 40X oil, 63X oil, 100X oil; adapters for 35 mm in diameter plates and adapter for well plates.

Person responsible: Dalila Pedro | dalila.pedro@i3s.up.pt

More information about standard operating procedures and technical information can be found in the Documents section.


Other resources
  • Bio-Plex Multiplex Immunoassay System (Bio-Plex 200 system)

This equipment is a suspension array system that allows the analysis of up to 100 biomolecules per sample, using color-coded magnetic beads (xMAP technology). Each bead is internally labeled with a distinct ratio of red and infrared dyes, making it possible that in a single well you can read multiple analytes.

We also have available an automatic magnetic washer and a microplate shaker, so the whole kit process can be done at our facility. The individual kits to be run in the equipment have to be acquired by the users. This system is compatible with most kits that use xMAP technology.

Person responsible: Dalila Pedro | dalila.pedro@i3s.up.pt



  • Critical point dryer (CPD 7501, Polaron Range)

Our critical point dryer (CPD 7501, Polaron Range) is used for not only dehydrating biological tissues but also any other material that requires dehydrating, prior to examination in the Scanning Electron Microscope to better preserve the sample’s ultrastructure. This preservation is obtained considering that during the critical point temperature of a certain fluid, there is no apparent difference between the liquid and gas state, reducing the surface tension to zero. The best suitable inert fluid, whose critical point is just above ambient and does not cause temperature damage to the sample, is CO2.

Person responsible: Dalila Pedro | dalila.pedro@i3s.up.pt


  • Equipment for Hypoxia Assays - Binder Incubator (CB170)

Hypoxia is a physiological state characterized by decreased oxygen levels and plays a role in embryonic development and is also physiologically normal for some adult tissues which maintain a hypoxic environment (e.g., bone marrow microdomains and thymus). Nevertheless, oxygen deficiency affects cellular functions and disrupts various biological processes including cell proliferation, differentiation, angiogenesis, metabolism, and pH homeostasis. Therefore, hypoxia is associated with various pathophysiological conditions including pulmonary diseases, congenital heart disease, cerebral ischemia, and cancer.

Person responsible: Dalila Pedro | dalila.pedro@i3s.up.pt

Offline workstations and software available

Offline workstations

The corresponding schedulers for the offline workstations can be found here.  The software available in each computer is displayed in the information on the corresponding scheduler.

Equipment's specific software


Image processing and/or analysis software

Other Softwares


The Bioimaging is open to all academic researchers and industrial clients on a fee-for-service basis.


As part of our mission, we provide training and assistance on all of our equipment and on image processing and analysis.

New users should fulfill the Experimental Design Form and send it to the following email: b.image@ineb.up.pt.

Our staff also organizes different hands-on workshops related to the technologies available and participates in the organization of Scientific Conferences. More information can be found in the Education tab. 


Educational publications

  • Gomez-Lazaro, Maria; Haberthuer, David; Hlushchuk, Ruslan. "Computer tomography in Biomedicine". In A compendium of imaging modalities for biological & preclinical research as part of the IOP-IPEM ebook Series in Physics and Engineering in Medicine and Biology to be published by IOP Publishing Ltd which trades as IOP Publishing of Temple Circus, Temple Way, Bristol BS1 6HG, UK.. Reino Unido: IOP Publishing Ltd, 2020.
  • Maia, André; Gomez-Lazaro, Maria. "High-Content Microscopy". In A compendium of imaging modalities for biological & preclinical research as part of the IOP-IPEM ebook Series in Physics and Engineering in Medicine and Biology to be published by IOP Publishing Ltd which trades as IOP Publishing of Temple Circus, Temple Way, Bristol BS1 6HG, UK.. Bristol, Reino Unido: IOP Publishing Ltd, 2020.
  • Gomez-Lazaro, Maria; Aroso, Miguel. "Confocal microscopy in the life sciences". In Fluorescence Imaging and Biological Quantification, editado por Seruca, Raquel; Jasjit S. Suri; Sanches, Miguel J, 3-24. Boca Raton, Estados Unidos: CRC Press, 2017.
  • Gomez-Lazaro, Maria; Freitas, Ana; Ribeiro, Cristina C. "Confocal Raman microscopy. Imaging the chemistry". In Fluorescence Imaging and Biological Quantification, editado por Seruca, Raquel; Jasjit S. Suri; Sanches, Miguel J, 65-83. Boca Raton, Estados Unidos: CRC Press, 2017.



  • Freitas L, Bezerra A, Resende-Coelho A, Gomez-Lazaro M, Maciel L, Amorim T, Fernandes RJ, Fonseca H. Impact of Long-Term Swimming Exercise on Rat Femur Bone Quality. Biomedicines. 2023 Dec 22;12(1):35. doi: 10.3390/biomedicines12010035. PMID: 38255142; PMCID: PMC10813774.
  • Manubens-Gil L, Zhou Z, Chen H, Ramanathan A, Liu X, Liu Y, Bria A, Gillette T, Ruan Z, Yang J, Radojevi? M, Zhao T, Cheng L, Qu L, Liu S, Bouchard KE, Gu L, Cai W, Ji S, Roysam B, Wang CW, Yu H, Sironi A, Iascone DM, Zhou J, Bas E, Conde-Sousa E, Aguiar P, Li X, Li Y, Nanda S, Wang Y, Muresan L, Fua P, Ye B, He HY, Staiger JF, Peter M, Cox DN, Simonneau M, Oberlaender M, Jefferis G, Ito K, Gonzalez-Bellido P, Kim J, Rubel E, Cline HT, Zeng H, Nern A, Chiang AS, Yao J, Roskams J, Livesey R, Stevens J, Liu T, Dang C, Guo Y, Zhong N, Tourassi G, Hill S, Hawrylycz M, Koch C, Meijering E, Ascoli GA, Peng H. BigNeuron: a resource to benchmark and predict performance of algorithms for automated tracing of neurons in light microscopy datasets. Nat Methods. 2023 Jun;20(6):824-835. doi: 10.1038/s41592-023-01848-5. Epub 2023 Apr 17. PMID: 37069271.
  • Rodrigues PM, Sousa LG, Perrod C, Maceiras AR, Ferreirinha P, Pombinho R, Romera-Cárdenas G, Gomez-Lazaro M, Senkara M, Pistolic J, Cabanes D, Klein L, Saftig P, Alves NL. LAMP2 regulates autophagy in the thymic epithelium and thymic stroma-dependent CD4 T cell development. Autophagy. 2023 Feb;19(2):426-439. doi: 10.1080/15548627.2022.2074105. Epub 2022 May 19. PMID: 35535798; PMCID: PMC9851248. 
  • Pinto R, Matula J, Gomez-Lazaro M, Sousa M, Lobo A, Zikmund T, Kaiser J, Gomes JR. High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models. Sci Rep. 2022 Oct 19;12(1):17471. doi: 10.1038/s41598-022-21494-9. PMID: 36261475; PMCID: PMC9582034.
  • Conde-Sousa E, Vale J, Feng M, Xu K, Wang Y, Della Mea V, La Barbera D, Montahaei E, Baghshah M, Turzynski A, Gildenblat J, Klaiman E, Hong Y, Aresta G, Araújo T, Aguiar P, Eloy C, Polónia A. HEROHE Challenge: Predicting HER2 Status in Breast Cancer from Hematoxylin-Eosin Whole-Slide Imaging. J Imaging. 2022 Jul 31;8(8):213. doi: 10.3390/jimaging8080213. PMID: 36005456; PMCID: PMC9410129.
  • Pereira-Castro I, Garcia BC, Curinha A, Neves-Costa A, Conde-Sousa E, Moita LF, Moreira A. MCL1 alternative polyadenylation is essential for cell survival and mitochondria morphology. Cell Mol Life Sci. 2022 Mar 1;79(3):164. doi: 10.1007/s00018-022-04172-x. PMID: 35229202.
  • Summers HD, Gomes CP, Varela-Moreira A, Spencer AP, Gomez-Lazaro M, Pêgo AP, Rees P. Data-Driven Modeling of the Cellular Pharmacokinetics of Degradable Chitosan-Based Nanoparticles. Nanomaterials (Basel). 2021 Oct 3;11(10):2606. doi: 10.3390/nano11102606. PMID: 34685047; PMCID: PMC8538870.
  • Nelson G, Boehm U, Bagley S, Bajcsy P, Bischof J, Brown CM, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J, Ferrand A, Gelman L, Gheisari A, Hartmann H, Kukat C, Laude A, Mitkovski M, Munck S, North AJ, Rasse TM, Resch-Genger U, Schuetz LC, Seitz A, Strambio-De-Castillia C, Swedlow JR, Alexopoulos I, Aumayr K, Avilov S, Bakker GJ, Bammann RR, Bassi A, Beckert H, Beer S, Belyaev Y, Bierwagen J, Birngruber KA, Bosch M, Breitlow J, Cameron LA, Chalfoun J, Chambers JJ, Chen CL, Conde-Sousa E, Corbett AD, Cordelieres FP, Nery ED, Dietzel R, Eismann F, Fazeli E, Felscher A, Fried H, Gaudreault N, Goh WI, Guilbert T, Hadleigh R, Hemmerich P, Holst GA, Itano MS, Jaffe CB, Jambor HK, Jarvis SC, Keppler A, Kirchenbuechler D, Kirchner M, Kobayashi N, Krens G, Kunis S, Lacoste J, Marcello M, Martins GG, Metcalf DJ, Mitchell CA, Moore J, Mueller T, Nelson MS, Ogg S, Onami S, Palmer AL, Paul-Gilloteaux P, Pimentel JA, Plantard L, Podder S, Rexhepaj E, Royon A, Saari MA, Schapman D, Schoonderwoert V, Schroth-Diez B, Schwartz S, Shaw M, Spitaler M, Stoeckl MT, Sudar D, Teillon J, Terjung S, Thuenauer R, Wilms CD, Wright GD, Nitschke R. QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. J Microsc. 2021 Oct;284(1):56-73. doi: 10.1111/jmi.13041. Epub 2021 Aug 11. PMID: 34214188.
  • Freitas A, Aroso M, Barros A, Fernández M, Conde-Sousa E, Leite M, Carvalho ED, Ribeiro CC, Ferreira R, Pêgo AP, Vitorino R, Gomez-Lazaro M. Characterization of the Striatal Extracellular Matrix in a Mouse Model of Parkinson's Disease. Antioxidants (Basel). 2021 Jul 8;10(7):1095. doi: 10.3390/antiox10071095. PMID: 34356328; PMCID: PMC8301085.
  • Freitas A, Aroso M, Rocha S, Ferreira R, Vitorino R, Gomez-Lazaro M. Bioinformatic analysis of the human brain extracellular matrix proteome in neurodegenerative disorders. Eur J Neurosci. 2021 Jun;53(12):4016-4033. doi:10.1111/ejn.15316. Epub 2021 Jun 16. PMID: 34013613.
  • Neto L, Pinto N, Proença A, Amorim A, Conde-Sousa E. 4SpecID: Reference DNA Libraries Auditing and Annotation System for Forensic Applications. Genes (Basel). 2021 Jan 2;12(1):61. doi: 10.3390/genes12010061. PMID: 33401773; PMCID: PMC7824288.
  • La Barbera D, Polónia A, Roitero K, Conde-Sousa E, Della Mea V. Detection of HER2 from Haematoxylin-Eosin Slides Through a Cascade of Deep Learning Classifiers via Multi-Instance Learning. J Imaging. 2020 Aug 23;6(9):82. doi: 10.3390/jimaging6090082. PMID: 34460739; PMCID: PMC8321042.
  • Biselli S, Alencastre I, Tropmann K, Erdmann D, Chen M, Littmann T, Maia AF, Gomez-Lazaro M, Tanaka M, Ozawa T, Keller M, Lamghari M, Buschauer A, Bernhardt G. Fluorescent H2 Receptor Squaramide-Type Antagonists: Synthesis, Characterization, and Applications. ACS Med Chem Lett. 2020 Jul 20;11(8):1521-1528. doi: 10.1021/acsmedchemlett.0c00033. PMID: 32832018; PMCID: PMC7429974.
  • Sousa DM, Martins PS, Leitão L, Alves CJ, Gomez-Lazaro M, Neto E, Conceição F, Herzog H, Lamghari M. The lack of neuropeptide Y-Y1 receptor signaling modulates the chemical and mechanical properties of bone matrix. FASEB J. 2020 Mar;34(3):4163-4177. doi: 10.1096/fj.201902796R. Epub 2020 Jan 20. PMID: 31960508.
  • Leite M, Marques MS, Melo J, Pinto MT, Cavadas B, Aroso M, Gomez-Lazaro M, Seruca R, Figueiredo C. Helicobacter Pylori Targets the EPHA2 Receptor Tyrosine Kinase in Gastric Cells Modulating Key Cellular Functions. Cells. 2020 Feb 24;9(2):513. doi: 10.3390/cells9020513. PMID: 32102381; PMCID: PMC7072728.
  • Barros D , Conde-Sousa E , Gonçalves AM , Han WM , García AJ , Amaral IF , Pêgo AP . Engineering hydrogels with affinity-bound laminin as 3D neural stem cell culture systems. Biomater Sci. 2019 Nov 19;7(12):5338-5349. doi: 10.1039/c9bm00348g. PMID: 31620727; PMCID: PMC6864240.
  • Barros D, Parreira P, Furtado J, Ferreira-da-Silva F, Conde-Sousa E, García AJ, Martins MCL, Amaral IF, Pêgo AP. An affinity-based approach to engineer laminin-presenting cell instructive microenvironments. Biomaterials. 2019 Feb;192:601-611. doi: 10.1016/j.biomaterials.2018.10.039. Epub 2018 Dec 1. PMID:30509501.
  • Teixeira GQ, Pereira CL, Ferreira JR, Maia AF, Gomez-Lazaro M, Barbosa MA, Neidlinger-Wilke C, Goncalves RM. Immunomodulation of Human Mesenchymal Stem/Stromal Cells in Intervertebral Disc Degeneration: Insights From a Proinflammatory/Degenerative Ex Vivo Model. Spine (Phila Pa 1976). 2018 Jun 15;43(12):E673-E682. doi: 10.1097/BRS.0000000000002494. PMID: 29189572.
  • Seabra CL, Nunes C, Brás M, Gomez-Lazaro M, Reis CA, Gonçalves IC, Reis S, Martins MCL. Lipid nanoparticles to counteract gastric infection without affecting gut microbiota. Eur J Pharm Biopharm. 2018 Jun;127:378-386. doi: 10.1016/j.ejpb.2018.02.030. Epub 2018 Mar 7. PMID: 29524597.
  • Rocha S, Freitas A, Guimaraes SC, Vitorino R, Aroso M, Gomez-Lazaro M. Biological Implications of Differential Expression of Mitochondrial-Shaping Proteins in Parkinson's Disease. Antioxidants (Basel). 2017 Dec 21;7(1):1. doi: 10.3390/antiox7010001. PMID: 29267236; PMCID: PMC5789311.
  • Castro F, Pinto ML, Silva AM, Pereira CL, Teixeira GQ, Gomez-Lazaro M, Santos SG, Barbosa MA, Gonçalves RM, Oliveira MJ. Pro-inflammatory chitosan/poly(γ-glutamic acid) nanoparticles modulate human antigen-presenting cells phenotype and revert their pro-invasive capacity. Acta Biomater. 2017 Nov;63:96-109. doi: 10.1016/j.actbio.2017.09.016. Epub 2017 Sep 14. PMID: 28919508.
  • Lao-Peregrín C, Ballesteros JJ, Fernández M, Zamora-Moratalla A, Saavedra A, Gómez Lázaro M, Pérez-Navarro E, Burks D, Martín ED. Caffeine-mediated BDNF release regulates long-term synaptic plasticity through activation of IRS2 signaling. Addict Biol. 2017 Nov;22(6):1706-1718. doi: 10.1111/adb.12433. Epub 2016 Jul 25. PMID: 27457910; PMCID: PMC5697621.
  • Olivera-Severo D, Uberti AF, Marques MS, Pinto MT, Gomez-Lazaro M, Figueiredo C, Leite M, Carlini CR. A New Role for Helicobacter pylori Urease: Contributions to Angiogenesis. Front Microbiol. 2017 Sep 27;8:1883. doi: 10.3389/fmicb.2017.01883. PMID: 29021786; PMCID: PMC5623709.
  • Passmore JB, Pinho S, Gomez-Lazaro M, Schrader M. The respiratory chain inhibitor rotenone affects peroxisomal dynamics via its microtubule-destabilising activity. Histochem Cell Biol. 2017 Sep;148(3):331-341. doi:10.1007/s00418-017-1577-1. Epub 2017 May 18. PMID: 28523458; PMCID: PMC5539279.
  • Seabra CL, Nunes C, Gomez-Lazaro M, Correia M, Machado JC, Gonçalves IC, Reis CA, Reis S, Martins MCL. Docosahexaenoic acid loaded lipid nanoparticles with bactericidal activity against Helicobacter pylori. Int J Pharm. 2017 Mar 15;519(1-2):128-137. doi: 10.1016/j.ijpharm.2017.01.014. Epub 2017 Jan 11. PMID: 28088639.
  • Gomes CP, Varela-Moreira A, Leiro V, Lopes CDF, Moreno PMD, Gomez-Lazaro M, Pêgo AP. A high-throughput bioimaging study to assess the impact of chitosan-based nanoparticle degradation on DNA delivery performance. Acta Biomater. 2016 Dec;46:129-140. doi: 10.1016/j.actbio.2016.09.037. Epub 2016 Sep 26. PMID:27686038.
  • Teixeira GQ, Leite Pereira C, Castro F, Ferreira JR, Gomez-Lazaro M, Aguiar P, Barbosa MA, Neidlinger-Wilke C, Goncalves RM. Anti-inflammatory Chitosan/Poly-γ-glutamic acid nanoparticles control inflammation while remodeling extracellular matrix in degenerated intervertebral disc. Acta Biomater. 2016 Sep 15;42:168-179. doi: 10.1016/j.actbio.2016.06.013. Epub 2016 Jun 15. PMID: 27321188.
  • Ribeiro N, Costa-Pinheiro P, Henrique R, Gomez-Lazaro M, Pereira MP, Mansur AAP, Mansur HS, Jerónimo C, Sousa SR, Monteiro FJ. Comprehensive Analysis of Secreted Protein, Acidic and Rich in Cysteine in Prostate Carcinogenesis: Development of a 3D Nanostructured Bone-Like Model. J Biomed Nanotechnol. 2016 Aug;12(8):1667-78. doi: 10.1166/jbn.2016.2276. PMID: 29342345.
  • Aroso M, Ferreira R, Freitas A, Vitorino R, Gomez-Lazaro M. New insights on the mitochondrial proteome plasticity in Parkinson's disease. Proteomics Clin Appl. 2016 Apr;10(4):416-29. doi: 10.1002/prca.201500092. Epub 2016 Feb 24. PMID: 26749507.
  • Marques O, Porto G, Rêma A, Faria F, Cruz Paula A, Gomez-Lazaro M, Silva P, Martins da Silva B, Lopes C. Local iron homeostasis in the breast ductal carcinoma microenvironment. BMC Cancer. 2016 Mar 5;16:187. doi: 10.1186/s12885-016-2228-y. PMID: 26944411; PMCID: PMC4779214.
  • Silva AM, Varela-Moreira A, Pereira Gomes C, Molinos M, Leite M, Almeida M, Ribeiro D, Schrader M, Figueiredo C, Barbosa M, Gonçalves R, Almeida C, Pêgo A, Santos SG, Gomez-Lazaro M. Integrated Analysis of Biological Samples by Imaging Flow Cytometry. Microsc Microanal. 2015 Aug;21 Suppl 5:95-6. doi: 10.1017/S1431927615014282. PMID: 26227728.
  • Caires HR, Gomez-Lazaro M, Oliveira CM, Gomes D, Mateus DD, Oliveira C, Barrias CC, Barbosa MA, Almeida CR. Finding and tracing human MSC in 3D microenvironments with the photoconvertible protein Dendra2. Sci Rep. 2015 May 14;5:10079. doi: 10.1038/srep10079. PMID: 25974085; PMCID: PMC4431349.
  • Nunes JB, Peixoto J, Soares P, Maximo V, Carvalho S, Pinho SS, Vieira AF, Paredes J, Rego AC, Ferreira IL, Gomez-Lazaro M, Sobrinho-Simoes M, Singh KK, Lima J. OXPHOS dysfunction regulates integrin-β1 modifications and enhances cell motility and migration. Hum Mol Genet. 2015 Apr 1;24(7):1977-90. doi:10.1093/hmg/ddu612. Epub 2014 Dec 11. PMID: 25504047.
  • Costa-Almeida R, Gomez-Lazaro M, Ramalho C, Granja PL, Soares R, Guerreiro SG. Fibroblast-endothelial partners for vascularization strategies in tissue engineering. Tissue Eng Part A. 2015 Mar;21(5-6):1055-65. doi: 10.1089/ten.TEA.2014.0443. Epub 2014 Dec 11. PMID: 25340984; PMCID: PMC4356233. 
  • Lopes CD, Gomez-Lazaro M, Pêgo AP. Seeing is believing but quantifying is deciding. Nanomedicine (Lond). 2015;10(15):2307-10. doi: 10.2217/nnm.15.101. Epub 2015 Jul 31. PMID: 26227661.


Our staff organizes and participates in different hands-on workshops related to the technologies available at our Platform.



  • Hands-on workshop on Image Analysis for Imaging flow Cytometry, June 6th-8th, 2017
  • Hands-on workshop on Image Analysis for Imaging flow Cytometry, September 28th-30th, 2016



  • ASTROTECH training school. "Seeing is believing, quantifying is deciding", September 26th- 28th, 2022

ASTROTECH main objective is to create and develop the field of Glial Engineering, providing a consistent range of tools to record, study, and manipulate astrocytes in the healthy and diseased brain. 

This hands-on training school aimed at introducing the most commonly used techniques to culture and imaging 3D models. We focused on the challenges of culturing 3D cultures. Imaging analysis was also addressed to improve the outcomes of your studies as “seeing is believing, quantifying is deciding”!


The Bioimaging is a node of the Portuguese Platform of Bioimage (PPBI), which constitutes a functional platform dedicated to promote the technical integration and centralized management of shared resources in bioimaging in Portugal.

We also contribute to Eurobioimaging through the PPBI node.